- Email: [email protected]
KRAS, BRAF and TP53 mutations in Romanian colorectal cancer patients
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Abstracts / Journal of Biotechnology 231S (2016) S4–S109
To obtain vegetable juices, fresh plants were minced and homogenised in distilled water and phosphate buffer solution pH 7.2. The sterile aqueous and phosphate buffer filtered juices were inoculated with S. xylosus 108 CFU/ml and incubated at 37 ◦ C. The nitrate-nitrite conversion was evaluated every hour for 10 h using spectrophotometric methods. All juices obtained from Iceberg and Romaine lettuce showed significant nitrate concentrations. The highest conversion rate of nitrates to nitrites was recorded for lettuce juices obtained in phosphate buffer pH 7.2, after 9 h of fermentation. The fermented juice obtained from Romaine lettuce in phosphate buffer pH 7.2 showed the highest nitrites concentration (72.95 ppm). Iceberg and Romaine lettuce juices represent important sources of natural nitrates. The bacterial nitrate reductase’s activity is pHdependent. Acknowledgement: This work was carried out through Partnerships in priority areas Program – PN II, implemented with the support of MEN – UEFISCDI (Romania), project nr. 149/2014. http://dx.doi.org/10.1016/j.jbiotec.2016.05.325 Research regarding the precision agriculture practiced in Romania Camelia Manescu ∗ , Nicoleta Mateoc Sîrb, Ada Flavia Cristina, Attila Toth, Adrian Gavruta Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timisoara, Romania E-mail address: ada fl[email protected] (C. Manescu). Currently, in Romania, there is a great diversity of farming systems, of which the most popular systems are: conventional, sustainable, biological, organic and biodynamic. As the impact of conventional farming is manifested by negative actions on key natural resources, it shows up the necessity of using farming systems that protect the environment. Choosing the agriculture system is conditioned by the technical equipment, by the level of professional knowledge, but also by education and consideration for the environment of all those working in this field. Precision farming requires the use of various technological tools as: Global Positioning Systems (GPS), Remote Sensor Systems (Remote Sensing), Satellite and/or aerial imagery, along with Geographic Information Systems (GIS) in order to obtain as clear and accurate information as possible, regarding the agricultural production. The specialized studies are conducted within research center called “Centre of Excellence for Sustainable Rural Development of Romania” within BUASVM “King Michael I of Romania from Timisoara”, and the authors wanted to highlight the advantages of this system of agriculture compared to the conventional agriculture system. In conclusion, the authors consider that the precision farming has the potential to improve the profitability and sustainability of the land, by having positive effects on the agricultural production such as: reducing costs and increasing efficiency in the use of the production factors, improving the control systems, managing the inputs in the crop production and not least the impact on the environment. http://dx.doi.org/10.1016/j.jbiotec.2016.05.326
KRAS, BRAF and TP53 mutations in Romanian colorectal cancer patients Daniela Murarasu 1,∗ , Liliana Puiu 1 , Corina Mihalcea 1 , Sabin Cinca 1 , Laurentiu Simion 2 1
Institute of Oncology, Carcinogenesis and Molecular Biology Department, Bucharest, Romania 2 Institute of Oncology, Medical Oncology I Department, Bucharest, Romania E-mail address: [email protected] (D. Murarasu). KRAS, BRAF and TP53 genes are main contributors to colorectal carcinogenesis. The objective of this study was to analyze the KRAS, BRAF and TP53 genes mutational spectra and frequency in Romanian CRC patients. Tumor specimens were obtained from 63 patients who underwent tumor resection. DNA extracted from fresh tumor fragments was analyzed by direct sequencing for mutations in KRAS (exons 2, 3 and 4), BRAF (exon 15) and TP53 (exons 2–11) genes. In total, 39.7% of patients had a mutant KRAS genotype, 14.3% harbored BRAF mutations, and 46% carried TP53 mutations. The exon 2 of KRAS had a high mutation rate (80.0%) while exons 3 and 4 showed a lower frequency of mutations (4% and 16% respectively). V600E hotspot mutation counted for 77.8% of BRAF mutations. 89.7% of TP53 mutations were clustered in exons 4–8. Among the 29 TP53 mutated samples, 37.9% carried KRAS mutations and 20.7% had BRAF mutations. Two patients with mutations in codon 594 of the BRAF gene simultaneously carried mutations in TP53 and KRAS genes. Mutation distribution of TP53 gene was higher in distal tumors. In KRAS mutations were more frequent in proximal and rectal tumors, while BRAF mutations were frequent in proximal colon cancers. Assessment of TP53, KRAS and BRAF mutational spectra may help the development of personalized therapeutic strategies. http://dx.doi.org/10.1016/j.jbiotec.2016.05.327 Phytobiotics solutions in pig feed Olimpia Colibar, Emil Tîrziu, Claudia Sala, Ionela Hotea ∗ , Monica Seres, Alexandra Hobicean Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania”, Faculty of Veterinary Medicine, Department of Animal Production and Veterinary Public Health, Aradului Street No. 119, 300645 Timisoara, Romania E-mail address: alexandra [email protected] (I. Hotea). Phytogenic feed additives are seen as one of the top solution in animal nutrition once the ban for the most antibiotics growth promoters was imposed. Their mechanisms of action, other than those of antibiotics, rely on a variety of substances intended to ensure their protection and survival in nature. Carob tannins inhibit pathogenic bacteria and fungal microorganisms in the gut. Thyme can improve productive performances of animals. Phytogenic product DYS I administered in fattening pigs diet for three months (1 kg/t of feed), led to an increase in body mass of 3.83%. Feed conversion rate (FCR kg/kg) was reduced from 3.04 to 2.91. There was a general improvement in animals’ health and appearance, the number of diarrhea cases was lower (with 8%), occasional other diseases had a favorable evolution. http://dx.doi.org/10.1016/j.jbiotec.2016.05.328
Abstracts / Journal of Biotechnology 231S (2016) S4–S109
To obtain vegetable juices, fresh plants were minced and homogenised in distilled water and phosphate buffer solution pH 7.2. The sterile aqueous and phosphate buffer filtered juices were inoculated with S. xylosus 108 CFU/ml and incubated at 37 ◦ C. The nitrate-nitrite conversion was evaluated every hour for 10 h using spectrophotometric methods. All juices obtained from Iceberg and Romaine lettuce showed significant nitrate concentrations. The highest conversion rate of nitrates to nitrites was recorded for lettuce juices obtained in phosphate buffer pH 7.2, after 9 h of fermentation. The fermented juice obtained from Romaine lettuce in phosphate buffer pH 7.2 showed the highest nitrites concentration (72.95 ppm). Iceberg and Romaine lettuce juices represent important sources of natural nitrates. The bacterial nitrate reductase’s activity is pHdependent. Acknowledgement: This work was carried out through Partnerships in priority areas Program – PN II, implemented with the support of MEN – UEFISCDI (Romania), project nr. 149/2014. http://dx.doi.org/10.1016/j.jbiotec.2016.05.325 Research regarding the precision agriculture practiced in Romania Camelia Manescu ∗ , Nicoleta Mateoc Sîrb, Ada Flavia Cristina, Attila Toth, Adrian Gavruta Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timisoara, Romania E-mail address: ada fl[email protected] (C. Manescu). Currently, in Romania, there is a great diversity of farming systems, of which the most popular systems are: conventional, sustainable, biological, organic and biodynamic. As the impact of conventional farming is manifested by negative actions on key natural resources, it shows up the necessity of using farming systems that protect the environment. Choosing the agriculture system is conditioned by the technical equipment, by the level of professional knowledge, but also by education and consideration for the environment of all those working in this field. Precision farming requires the use of various technological tools as: Global Positioning Systems (GPS), Remote Sensor Systems (Remote Sensing), Satellite and/or aerial imagery, along with Geographic Information Systems (GIS) in order to obtain as clear and accurate information as possible, regarding the agricultural production. The specialized studies are conducted within research center called “Centre of Excellence for Sustainable Rural Development of Romania” within BUASVM “King Michael I of Romania from Timisoara”, and the authors wanted to highlight the advantages of this system of agriculture compared to the conventional agriculture system. In conclusion, the authors consider that the precision farming has the potential to improve the profitability and sustainability of the land, by having positive effects on the agricultural production such as: reducing costs and increasing efficiency in the use of the production factors, improving the control systems, managing the inputs in the crop production and not least the impact on the environment. http://dx.doi.org/10.1016/j.jbiotec.2016.05.326
KRAS, BRAF and TP53 mutations in Romanian colorectal cancer patients Daniela Murarasu 1,∗ , Liliana Puiu 1 , Corina Mihalcea 1 , Sabin Cinca 1 , Laurentiu Simion 2 1
Institute of Oncology, Carcinogenesis and Molecular Biology Department, Bucharest, Romania 2 Institute of Oncology, Medical Oncology I Department, Bucharest, Romania E-mail address: [email protected] (D. Murarasu). KRAS, BRAF and TP53 genes are main contributors to colorectal carcinogenesis. The objective of this study was to analyze the KRAS, BRAF and TP53 genes mutational spectra and frequency in Romanian CRC patients. Tumor specimens were obtained from 63 patients who underwent tumor resection. DNA extracted from fresh tumor fragments was analyzed by direct sequencing for mutations in KRAS (exons 2, 3 and 4), BRAF (exon 15) and TP53 (exons 2–11) genes. In total, 39.7% of patients had a mutant KRAS genotype, 14.3% harbored BRAF mutations, and 46% carried TP53 mutations. The exon 2 of KRAS had a high mutation rate (80.0%) while exons 3 and 4 showed a lower frequency of mutations (4% and 16% respectively). V600E hotspot mutation counted for 77.8% of BRAF mutations. 89.7% of TP53 mutations were clustered in exons 4–8. Among the 29 TP53 mutated samples, 37.9% carried KRAS mutations and 20.7% had BRAF mutations. Two patients with mutations in codon 594 of the BRAF gene simultaneously carried mutations in TP53 and KRAS genes. Mutation distribution of TP53 gene was higher in distal tumors. In KRAS mutations were more frequent in proximal and rectal tumors, while BRAF mutations were frequent in proximal colon cancers. Assessment of TP53, KRAS and BRAF mutational spectra may help the development of personalized therapeutic strategies. http://dx.doi.org/10.1016/j.jbiotec.2016.05.327 Phytobiotics solutions in pig feed Olimpia Colibar, Emil Tîrziu, Claudia Sala, Ionela Hotea ∗ , Monica Seres, Alexandra Hobicean Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania”, Faculty of Veterinary Medicine, Department of Animal Production and Veterinary Public Health, Aradului Street No. 119, 300645 Timisoara, Romania E-mail address: alexandra [email protected] (I. Hotea). Phytogenic feed additives are seen as one of the top solution in animal nutrition once the ban for the most antibiotics growth promoters was imposed. Their mechanisms of action, other than those of antibiotics, rely on a variety of substances intended to ensure their protection and survival in nature. Carob tannins inhibit pathogenic bacteria and fungal microorganisms in the gut. Thyme can improve productive performances of animals. Phytogenic product DYS I administered in fattening pigs diet for three months (1 kg/t of feed), led to an increase in body mass of 3.83%. Feed conversion rate (FCR kg/kg) was reduced from 3.04 to 2.91. There was a general improvement in animals’ health and appearance, the number of diarrhea cases was lower (with 8%), occasional other diseases had a favorable evolution. http://dx.doi.org/10.1016/j.jbiotec.2016.05.328