Outline of volumes I, II, III, IV, and V

Outline

of Volumes

I, I I , I I I , I V , a n d

V

VOLUME I PREPARATION AND ASSAY OF ENZYMES Section I. General P r e p a r a t i v e Procedures A. Tissue Slice Technique. B. Tissue Homogenates. C. Fractionation of Cellular Components. D. Methods of Extraction of Enzymes. E. Protein Fractionation. F. Preparation of Buffers. Section II. E n z y m e s of Carbohydrate Metabolism A. Polysaccharide Cleavage and Synthesis.B. Disaccharide, Hexoside, and Glucuronide Metabolism. C. Metabolism of Hexoses. D. Metabolism of Pentoses. E. Metabolism of Three-Carbon Compounds. F. Reactions of Two-Carbon Compounds. G. Reactions of

Formate. Section I I I . E n z y m e s of Lipid Metabolism A. Fatty Acid Oxidation. B. Acyl Activation and Transfer. C. Lipases and Esterases. D. Phospholipid and Steroid Enzymes. Section IV. E n z y m e s of Citric Acid Cycle VOLUME II PREPARATION AND ASSAY OF ENZYMES Section I. E n z y m e s of Protein Metabolism A. Protein Hydrolyzing Enzymes. B. Enzymes in Amino Acid Metabolism (General). C. Specific Amino Acid Enzymes. D. Peptide Bond Synthesis. E. Enzymes in Urea Synthesis. F. Ammonia Liberating Enzymes. G. Nitrate Metabolism. Section I I . E n z y m e s of Nucleic Acid Metabolism A. Nucleases. B. Nucleosidases. C. Deaminases. D. 0xidases. E. Nueleotide Synthesis. Section I I I . E n z y m e s in P h o s p h a t e Metabolism A. Pbosphomonoesterases. B. Phosphodiesterases. C. Inorganic Pyro- and Poly-phosphatases. D. ATPases. E. Phosphate-Transferring Systems. Section IV. E n z y m e s in Coenzyme and Vitamin Metabolism A. Synthesis and Degradation of Vitamins. lB. Phosphorylation of Vitamins. C. Coenzyme Synthesis and Breakdown. Section V. R e s p i r a t o r y E n z y m e s A. PyHdine Nucleotide-Linked, Including Flavoproteins. B. Iron-Porphyrins. C. Copper Enzymes. D. Unclassified. x~

xxii

OUTLINE OF VOLUMES I~ II~ III~ IV~ AND V

VOLUME PREPARATION

AND

III

ASSAY

OF SUBSTRATES

Section I. Carbohydrates A. Polysaecharides. B. Monosaecharides. C. Sugar Phosphates and Related Compounds. D. Unphesphorylated Intermediates and Products of Fermentation and Respiration. Section If. Lipids and Steroids A. Isolation and Determination of Lipids and Higher Fatty Acids. B. Preparation and Analysis of Phospholipids and Derivatives. C. Fractionation Procedures for Higher and Lower Fatty Acids. D. Preparation and Assay of Cholesterol and Ergosterol. Section III. Citric Acid Cycle C o m p o n e n t s A. Chromatographic Analyses of Organic Acids. B. Specific Procedures for Individual Compounds. Section IV. Proteins and Derivatives A. General Procedures for Determination of Proteins and Amino Acids. B. General Procedures for Preparation of Peptides and Amino Acids. C. Specific Procedures for Isolation and Determination of Individual Amino Acids. Section V. Nucleic Acids and Derivatives A. Determination, Isolation, and Characterization of Nucleic Acids. B. Determination, Isolation, Characterization, and Synthesis of Nucleotides and Nucleosides. Section VI. Coenzymes and Related Phosphate Compounds A. General Procedures for Isolation, Determination, and Characterization of Phosphorus Compounds. B. Specific Procedures for N-Phosphates and Individual Coenzymes. Section V I I . Determination of Inorganic Compounds

V O L U M E IV SPECIAL TECHNIQUES FOR THE ENZYMOLOGIST Section I. Techniques for Characterization of Proteins (Procedures and Interpretations) A. Electrophoresis; Macro and Micro. B. Ultracentrifugation and Related Techniques (Diffusion, Viscosity) for Molecular Size and Shape. C. Infra-red Spectrophotometry. D, X-ray Diffraction. E. Light Scattering Measurements. F. Flow" Birefringence. G, Fluorescence Polarization and Other Fluorescence Techniques. H. The Solubility Method for Protein Purity. I. Determindtion of Amino Acid Sequence in Proteins. ,I.Determination of Essential Groups for Enzyme Activity.

°.,

OUTLINE OF VOLUMES I~ II~ III~ IV, AND V

XXIll

Section II. Techniques for Metabolic Studies A. Measurement of Rapid Reaction Rates; Techniques and Applications, Including Determination of Spectra of Cytochromes and Other Electron Carriers in Respiring Cells. B. Use of Artificial Electron Acceptors in the Study of Dehydrogenases. C. Use of Percolation Technique for the Study of the Metabolism of Soil Microorganisms. D. Methods for Study of the Hill Reaction. E. Methods for Measurement of Nitrogen Fixation. F. Cytochemistry. Section I I I . Techniques for Isotope Studies A. The Measurement of Isotopes. B. The Synthesis and Degradation of Labeled Compounds (Including Application to Metabolic Studies): Monosaccharides and Polysaccharides; Citric Acid Cycle Intermediates; Glycolic, Glyoxylic and Oxalic Acids; Purines and Pyrimidines; Porphyrins; Amino Acids and Proteins; Steroids; Methylated Compounds and Derivatives; Sulfur Compounds; Fatty Acids; Phospholipids; Coenzymes; Iodinated Compounds; Intermediates of Photosynthesis; OllLabeled Phosphorus Compounds. VOLUME V PREPARATION AND ASSAY OF ENZYMES Section I. General Preparative Procedures A. Column Chromatography of Proteins. B. Preparative Electrophoresis. C. Preparation and Solubilization of Particles (Bacterial, Mammalian, and Higher plant). D. Mammalian Cell Culture. E. Protoplasts. Section II. E n z y m e s of C a r b o h y d r a t e Metabolism A. Polysaceharide Cleavage and Synthesis. B. Disaccharide, Hexoside and Glucuronide Metabolism. C. Metabolism of Hexoses, Pentoses and 3-Carbon Compounds. D. Hexosamine and Sialic Acid Metabolism. E. Aromatic Ring Synthesis. Section I I I . E n z y m e s of Lipid Metabolism A. Fatty Acid Synthesis and Breakdown. B. Acid Activating Enzymes. C. Phospholipid Synthesis and Breakdown. D. Steroid Metabolism. Section IV. E n z y m e s of Citric Acid Cycle A. Krebs Cycle. B. Krebs-Kornberg Cycle. C. Related Enzymes. Section V. E n z y m e s of Protein Metabolism A. Proteolytic Enzymes. B. Amino Acid Dehydrogenases and Transaminases. C. Amino Acid Activating Enzymes. D. Other Enzymes of Amino Acid Breakdown and Synthesis. E. Enzymes of Sulfur Metabolism.