The effects of fine suspended solids on coliform estimations in seawater using membrane filtration

888

E. BiologicalOceanography

OLR( 1981128(12~

during preparation for SEM;epifluorescence microscopy of wet preserved ceils is probably more accurate than SEM for determination of size and biovolume. Scripps Inst. of Oceanog.. La Jolla, Calif. 92093, USA.

81:6706 McGrath, S.M. and C.W. Sullivan, 1981. Ctmmmnity metabolism of a d e n ~ by from the Los ~ Harbor and southern California coastal waters. Mar. Biol., 62(2/3): 217-226.

81:6702 Gocke, K., R. Dawson and G. Liebezeit, 1981. A v s U ~ I ~ of d i m o ~ f r e e ~ to i ~ e m U ~ phic mJel~arlpm~B~. Mar. Biol., 62(2/3):209216.

Concentrations of dissolved total adenytates ( A M P + A D P + A T P ) were measured (2.8_+ 1.7 nmol/L); compartments and fluxes revolved in adenylate cycling were estimated, including eel] numbers, biomass, and uptake rates for m-situ microbial populations. Most of the AMP was taken up by organisms < 1.0/un. A model is proposed. Dept. of Biol. Sci., Univ. of So. Calif.. Los Angeles. Calif. 90007. USA. (mjj)

Concentrations of dissolved free glucose in 5 different aquatic biotopes ranged 4.99-9.63 /~g C/L determined chemically; determined microbiologically as Kt+ S,, they ranged 0.62-2.72 /~g C/L. The discrepancy was not due to methodological error. but was attributed to differences in availability of truly free, absorbed, or complexed dissolved free glucose, or to different specific labelling of these different glucose fractions. Inst. fur Meereskunde an der Univ. Kid, D-2300 Kiel l, FRG. (mjj) 81:6703 Iturriaga, R. and A. Zsolnay, 1981. TrlmMmmation

of~

These 'smallest of known marine organisms' passed through membrane filters of 0.40 and 0.23/jan and had an average volume of 0.04 /Lm3. (izs)

~ ~ ~ b y a M m r ~ ~ n .

Mar. Biol., 62(2/3):

125-129. '4C-labelled glycolic acid, arginine, glutamic acid, glycine, and glucose were rapidly taken up by natural marine bacteria; all except glycotic acid were transformed and excreted as extracellular metabolites (5-t0% of original label) within 72 hr. Dept. of Biol. Sci., Univ. of Southern Calif., Los Angeles, Calif. 90007, USA. (mjj) 81:6704 Iversen, Niels and T.H. Blackburn, 198I,

~ts.

81: 6707 Mishustina, I.E. and T.G. Kameneva, 1981. Bacterial cells of minimal size in the Bments Sea during polar night. Mikrobiologiya, 50(2):360-363. (In Russian, English abstract.)

Appl. environ. Microbiol., 4t(6):1295-

1300. Rates of methane oxidation in cores of inshore marine sediment (Ju~nd, Denmark) r a n ~ from 3.34 to 17.04 panel m-2day-!, varying:sc~onally and with depth. At most only 0,06% of sutfate reduction could be accounted for by methane oxidation, Inst. of Ecol. and Genetics, Univ. of Aarhus, DK-8000 Aarhus C., Denmark, (mjj)

m:67os Krs 2I(1):203'218. last: of Ocean0g. and Fisheries, Split, Yugoslavia.

81:6708 Mitskevich, I.N. and E.P. Nikitina, 198I. Layers with lncremed concentrations of m ~ in the lndhn Ocean, Mikrobiologiya, 50(2):364-370. fin Russian, English abstract.) Examination of >700 water samples (from 0-5000 m) showed microorganism density decreasing with depth; however, secondary maxima occurred at oceanic structural zone interfaces (300-500 m and 1000-1500120001m). (ahm) 81:67~ Patrick, F.M. and T.H. Kendrick, 1980. ~ effects of fine ~ studs on coliform ~ in seawater using membrane filtration. N.Z. Jl Sci., 23(4):329-333. More than 7 mg of fine suspended solids (<~200 #m) deposited on a membrane markedly affected cell, form bacterial colony growth and integrity, rendering these membranes uncoun~ble. A smaller amount of finer sediment (<44 /an) produced the same effect. No correlation was observed between suspended solids concentration and the ratio of membrane filter to most probable number coliform bacteria counts when membranes were countable. Ministry of Works and Development, P,O. Box 47008, Trentham, Wellington, New Zealand. 81:6710 Romano, J.-C. and R. Daumas, 1981. A ~ i n e