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Two-dimensional electrophoresis and immunological techniques
Journal of ImmunologicalMethods, 121 (1989)147-148 Elsevier JIM
147
05234 Book reviews
Dunbar, B.S., Two-Dimensional Electrophoresis and Immunological Techniques, xvi + 372 pp. Plenum Press, New York, 1987. US $59.50, ISBN 0-306-42439-8 This book authored by a staff member of the Baylor College of Medicine in Houston, Texas, represents an extensive laboratory manual of polyacrylamide gel electrophoresis (PAGE). It consists of eleven chapters and sixteen appendices. Chapter 1 explains the basic principles of electrophoresis" both in free solution and in support media, with the emphasis on PAGE. Special sections are devoted to the choice of buffers for electrophoresis, isoelectric focusing, and high-resolution two-dimensional PAGE. Chapter 2 describes some of the applications of PAGE such as the estimation of the purity or complexity of a sample, the determination of molecular weight and charge heterogeneity of proteins, peptide mapping, obtaining peptides with unique amino acid sequences for the preparation of oligonucleoticie probes, and the preparation of highly purified proteins or peptides for antibody production and characterization. Chapter 3 deals with sample preparation for PAGE; it presents method~ for protein quantitation, solub'flization, and fra.~tionation. Protein detection in polyacrylamide gel by different staining methods and the analysis of glycoproteins by high-resolution 2D-PAGE are the subjects of chapters 4 and 5. Illustrated therein is the use of MW markers. Protein radiolabelling techniques
are discusse:l in chapter 6 and autoradiography in chapter 7. Chapter 8 describes procedures for raising, purifying, and detecting antibodies. It inchides some basic information on the subjects, presumably for non-immunologists. After a survey of chromatographic methods for immunoglobulin fractionation, Ig analysis by one-dimensional SDS-PAGE and monoclonal antibody characterization are depicted. Immunodiffusion and immunoelectrophoretic techniques are also described and ELISA and immunoblotting shown schematically and evaluated. The remaining chapters offer practical advice on laboratory photography, reagent quality, equipment maintenance, and overcoming the problems encountered in 2D-PAGE, and discuss recent advances in improving the resolution and reproducibility of protein analysis by 2D-PAGE. The appendices that follow contain detailed protocols for different techniques beginning with protein assays, further additional literature references and the addresses of suppliers of equipment. A comprehensive subject index closes the volume. The book is provided with numerous high-quality illustrations including photographs of electropherograms, some in colour. The easily readable print, glossy paper, and compact size of this manual make it eminently suitable for routine use in the labo;atory. F. Borek
JIM 05243 Olszewski, W.L., In Vivo Migration of Immune Cells, 264 pp., illus. CRC Press, Boca Raton, FL, 1987. $170.00, ISBN 0-8493-5076-x
This is an important publication in a relatively underdeveloped branch of immunology - the study of lymphocyte traffic in the body, of the factors
0022-1759/89/$03.50© 1989 ElsevierSciencePublishers B.V.(BiomedicalDivision)
147
05234 Book reviews
Dunbar, B.S., Two-Dimensional Electrophoresis and Immunological Techniques, xvi + 372 pp. Plenum Press, New York, 1987. US $59.50, ISBN 0-306-42439-8 This book authored by a staff member of the Baylor College of Medicine in Houston, Texas, represents an extensive laboratory manual of polyacrylamide gel electrophoresis (PAGE). It consists of eleven chapters and sixteen appendices. Chapter 1 explains the basic principles of electrophoresis" both in free solution and in support media, with the emphasis on PAGE. Special sections are devoted to the choice of buffers for electrophoresis, isoelectric focusing, and high-resolution two-dimensional PAGE. Chapter 2 describes some of the applications of PAGE such as the estimation of the purity or complexity of a sample, the determination of molecular weight and charge heterogeneity of proteins, peptide mapping, obtaining peptides with unique amino acid sequences for the preparation of oligonucleoticie probes, and the preparation of highly purified proteins or peptides for antibody production and characterization. Chapter 3 deals with sample preparation for PAGE; it presents method~ for protein quantitation, solub'flization, and fra.~tionation. Protein detection in polyacrylamide gel by different staining methods and the analysis of glycoproteins by high-resolution 2D-PAGE are the subjects of chapters 4 and 5. Illustrated therein is the use of MW markers. Protein radiolabelling techniques
are discusse:l in chapter 6 and autoradiography in chapter 7. Chapter 8 describes procedures for raising, purifying, and detecting antibodies. It inchides some basic information on the subjects, presumably for non-immunologists. After a survey of chromatographic methods for immunoglobulin fractionation, Ig analysis by one-dimensional SDS-PAGE and monoclonal antibody characterization are depicted. Immunodiffusion and immunoelectrophoretic techniques are also described and ELISA and immunoblotting shown schematically and evaluated. The remaining chapters offer practical advice on laboratory photography, reagent quality, equipment maintenance, and overcoming the problems encountered in 2D-PAGE, and discuss recent advances in improving the resolution and reproducibility of protein analysis by 2D-PAGE. The appendices that follow contain detailed protocols for different techniques beginning with protein assays, further additional literature references and the addresses of suppliers of equipment. A comprehensive subject index closes the volume. The book is provided with numerous high-quality illustrations including photographs of electropherograms, some in colour. The easily readable print, glossy paper, and compact size of this manual make it eminently suitable for routine use in the labo;atory. F. Borek
JIM 05243 Olszewski, W.L., In Vivo Migration of Immune Cells, 264 pp., illus. CRC Press, Boca Raton, FL, 1987. $170.00, ISBN 0-8493-5076-x
This is an important publication in a relatively underdeveloped branch of immunology - the study of lymphocyte traffic in the body, of the factors
0022-1759/89/$03.50© 1989 ElsevierSciencePublishers B.V.(BiomedicalDivision)