- Email: [email protected]
112. Attachment avoidance predicts inflammatory response to marital conflict
Brain, Behavior, and Immunity 22 (2008) 36–42
Contents lists available at ScienceDirect
Brain, Behavior, and Immunity journal homepage: www.elsevier.com/locate/ybrbi
PNIRS 2008 Abstracts 111–131 111. The curcumin analog, UBS-109 decreases peripheral expression of LPS-induced inflammatory cytokines Anlys Olivera *, Fang Hu, Andrew P. Brown, Hyunsuk Shim, Dennis C. Liotta, Andrew H. Miller, Thaddeus W.W. Pace Emory University * Corresponding author. E-mail: [email protected] Prior studies suggest that the inflammatory signaling molecule, nuclear factor-kappa B (NF-kB), plays a key role in transmitting peripheral cytokine signals to the brain. Therefore, NF-kB represents an attractive therapeutic target for developing drugs to prevent cytokine induced behavioral changes. Current work in our laboratories is focused on the development of novel compounds structurally related to curcumin, (an active ingredient in the Indian spice, tumeric), which has been shown to have anti-inflammatory and anti-depressant properties. Data demonstrate that a water soluble analog of curcumin, UBS-109, has potent inhibitory effects on NF-kB DNA-binding in vitro. In the current study, we tested the ability of UBS-109 to reduce lipopolysaccharide (LPS)-induced expression of pro-inflammatory cytokines that are regulated by NF-kB pathways in peripheral tissues including the spleen. Young adult C57/BL6 (23 g) male mice (n = 3/group, N = 24) were treated with UBS-109 (150 mg/kg) or vehicle (90% methyl cellulose [.5%] + 10% PEG200) orally prior to LPS administration (30ug/mouse). Mice were then sacrificed 6 or 24 h after LPS treatment, and spleens were collected for cytokine expression analysis using real-time PCR. Treatment with LPS increased the expression of IL-1 alpha and beta, IL-6, TNF-alpha and IFN-gamma. Administration of UBS-109 prior to LPS injection significantly reduced mRNA expression of these cytokines at both 6 and 24 h post injection. These data indicate that oral administration of UBS-109 effectively reduces the expression of pro-inflammatory cytokines that are regulated by activation of NF-kB pathways. This novel compound may therefore block behavioral abnormalities induced by activation of peripheral cytokine signaling pathways including major depression. doi:10.1016/j.bbi.2008.04.114
112. Attachment avoidance predicts inflammatory response to marital conflict Jean-Philippe Gouin a,b,*, Ronald Glaser b,c,d, William Malarkey b,c,d,e, Timothy Loving f, Janice Kiecolt-Glaser a,b,e a
Department of Psychology, The Ohio State University, USA Institute for Behavioral Medicine Research, Ohio State University College of Medicine, USA c Department of Internal Medicine, Ohio State University College of Medicine, USA d Department of Molecular Virology, Immunology, and Medical Genetics, Ohio State University College of Medicine, USA b
e
Department of Psychiatry, Ohio State University College of Medicine, USA f Department of Human Ecology, The University of Texas at Austin, USA * Corresponding author. E-mail: [email protected] Marital stress has been associated with immune dysregulation, including increased production of circulating proinflammatory cytokines interleukin-6 (IL-6) and tumor necrosis-±; (TNF-±;). Attachment style is an individual difference in one’s expectation about the availability and responsiveness of others in intimate relationships. Attachment avoidance, one dimension of attachment style representing difficulties in relying on and opening up to others, has been related to increased physiological stress reactivity. We hypothesized that individuals who exhibited higher levels of attachment avoidance would show an exacerbated inflammatory response to a marital conflict, but not to a socially supportive marital interaction, compared to individuals with lower levels of attachment avoidance. During the first of two 24-h admissions to a hospital research unit, 40 married couples had a structured social support interaction; during the second visit they discussed a marital disagreement. Results from blood samples drawn at baseline, 4, 7, and 22 h showed that attachment avoidance was not related to serum IL-6 and TNF-±; levels during the social support visit. In contrast, during the conflict visit, husbands’ attachment avoidance predicted wives’ IL-6 baseline levels and husbands’ levels of IL-6 at baseline, 4, and 7 h. Wives’ attachment avoidance predicted husband’s baseline IL-6 and TNF±; at baseline and 4 h. During the conflict visit, the wives’ attachment avoidance was associated with their own negative behaviors, while husband attachment avoidance was related to both spouses’ negative behaviors, suggesting a mechanism by which attachment style influences inflammatory response to marital conflict. doi:10.1016/j.bbi.2008.04.115
113. Perceived discrimination predicts elevated levels of fibrinogen in a national sample: Data from the Survey of Midlife in the United States (MIDUS) Elliot Friedman a,*, David Williams b, Burton Singer a,c, Carol Ryff a,d a
Institute on Aging, University of Wisconsin, Madison, WI, USA Harvard University, Cambridge, MA, USA c Office of Population Research, Princeton University, Princeton, NJ, USA d Psychology Department, University of Wisconsin-Madison, USA * Corresponding author. E-mail: [email protected] b
Perceived discrimination is considered a profound stressor that has been linked to a range of adverse health outcomes, but the biological processes mediating these associations are poorly understood. Given prior research linking chronic discrimination to coronary artery calcification, this study tested the hypothesis that perceived discrimination would predict increased serum concentra-
Contents lists available at ScienceDirect
Brain, Behavior, and Immunity journal homepage: www.elsevier.com/locate/ybrbi
PNIRS 2008 Abstracts 111–131 111. The curcumin analog, UBS-109 decreases peripheral expression of LPS-induced inflammatory cytokines Anlys Olivera *, Fang Hu, Andrew P. Brown, Hyunsuk Shim, Dennis C. Liotta, Andrew H. Miller, Thaddeus W.W. Pace Emory University * Corresponding author. E-mail: [email protected] Prior studies suggest that the inflammatory signaling molecule, nuclear factor-kappa B (NF-kB), plays a key role in transmitting peripheral cytokine signals to the brain. Therefore, NF-kB represents an attractive therapeutic target for developing drugs to prevent cytokine induced behavioral changes. Current work in our laboratories is focused on the development of novel compounds structurally related to curcumin, (an active ingredient in the Indian spice, tumeric), which has been shown to have anti-inflammatory and anti-depressant properties. Data demonstrate that a water soluble analog of curcumin, UBS-109, has potent inhibitory effects on NF-kB DNA-binding in vitro. In the current study, we tested the ability of UBS-109 to reduce lipopolysaccharide (LPS)-induced expression of pro-inflammatory cytokines that are regulated by NF-kB pathways in peripheral tissues including the spleen. Young adult C57/BL6 (23 g) male mice (n = 3/group, N = 24) were treated with UBS-109 (150 mg/kg) or vehicle (90% methyl cellulose [.5%] + 10% PEG200) orally prior to LPS administration (30ug/mouse). Mice were then sacrificed 6 or 24 h after LPS treatment, and spleens were collected for cytokine expression analysis using real-time PCR. Treatment with LPS increased the expression of IL-1 alpha and beta, IL-6, TNF-alpha and IFN-gamma. Administration of UBS-109 prior to LPS injection significantly reduced mRNA expression of these cytokines at both 6 and 24 h post injection. These data indicate that oral administration of UBS-109 effectively reduces the expression of pro-inflammatory cytokines that are regulated by activation of NF-kB pathways. This novel compound may therefore block behavioral abnormalities induced by activation of peripheral cytokine signaling pathways including major depression. doi:10.1016/j.bbi.2008.04.114
112. Attachment avoidance predicts inflammatory response to marital conflict Jean-Philippe Gouin a,b,*, Ronald Glaser b,c,d, William Malarkey b,c,d,e, Timothy Loving f, Janice Kiecolt-Glaser a,b,e a
Department of Psychology, The Ohio State University, USA Institute for Behavioral Medicine Research, Ohio State University College of Medicine, USA c Department of Internal Medicine, Ohio State University College of Medicine, USA d Department of Molecular Virology, Immunology, and Medical Genetics, Ohio State University College of Medicine, USA b
e
Department of Psychiatry, Ohio State University College of Medicine, USA f Department of Human Ecology, The University of Texas at Austin, USA * Corresponding author. E-mail: [email protected] Marital stress has been associated with immune dysregulation, including increased production of circulating proinflammatory cytokines interleukin-6 (IL-6) and tumor necrosis-±; (TNF-±;). Attachment style is an individual difference in one’s expectation about the availability and responsiveness of others in intimate relationships. Attachment avoidance, one dimension of attachment style representing difficulties in relying on and opening up to others, has been related to increased physiological stress reactivity. We hypothesized that individuals who exhibited higher levels of attachment avoidance would show an exacerbated inflammatory response to a marital conflict, but not to a socially supportive marital interaction, compared to individuals with lower levels of attachment avoidance. During the first of two 24-h admissions to a hospital research unit, 40 married couples had a structured social support interaction; during the second visit they discussed a marital disagreement. Results from blood samples drawn at baseline, 4, 7, and 22 h showed that attachment avoidance was not related to serum IL-6 and TNF-±; levels during the social support visit. In contrast, during the conflict visit, husbands’ attachment avoidance predicted wives’ IL-6 baseline levels and husbands’ levels of IL-6 at baseline, 4, and 7 h. Wives’ attachment avoidance predicted husband’s baseline IL-6 and TNF±; at baseline and 4 h. During the conflict visit, the wives’ attachment avoidance was associated with their own negative behaviors, while husband attachment avoidance was related to both spouses’ negative behaviors, suggesting a mechanism by which attachment style influences inflammatory response to marital conflict. doi:10.1016/j.bbi.2008.04.115
113. Perceived discrimination predicts elevated levels of fibrinogen in a national sample: Data from the Survey of Midlife in the United States (MIDUS) Elliot Friedman a,*, David Williams b, Burton Singer a,c, Carol Ryff a,d a
Institute on Aging, University of Wisconsin, Madison, WI, USA Harvard University, Cambridge, MA, USA c Office of Population Research, Princeton University, Princeton, NJ, USA d Psychology Department, University of Wisconsin-Madison, USA * Corresponding author. E-mail: [email protected] b
Perceived discrimination is considered a profound stressor that has been linked to a range of adverse health outcomes, but the biological processes mediating these associations are poorly understood. Given prior research linking chronic discrimination to coronary artery calcification, this study tested the hypothesis that perceived discrimination would predict increased serum concentra-