- Email: [email protected]
194: Association between LDL subclasses and carotid intima-media thickness in coronary artery disease
Multiple Risk Factors in Cardiovascular Disease—Abstracts
Research, Eindhoven, The Netherlands, 2Future Diagnostics, Wijchen, The Netherlands Objective: The goal of the present work is to develop a compact handheld device for the rapid and sensitive detection of biomarkers relevant to progression of atherosclerosis and risk stratification of Coronary Artery Disease patients in a point-of-care setting. Methods: The diagnostic test is based on a novel analytical technique that combines active magnetic particles, actuating magnets and a magnetic particle sensor. The complete system consists of a small, disposable cartridge containing 500 nm superparamagnetic particles and a handheld analyzer. A troponin test is reported as an example. Upon the addition of the sample, troponin binds to its antibodies conjugated to the magnetic particles. Actuating electromagnets move the particles through the sample for effective analyte capture. By applying magnetic forces, the particles are then transported with high speed to the sensor surface, which contains capture anti-troponin antibodies. After the particles are allowed to react with the sensor surface, non-specifically bound particles are quickly removed by applying a magnetic force oriented away from the sensor surface. Results: Tests can be performed with a turn-around time of 5 minutes in a sample volume of 25 μL with picomolar sensitivity. Conclusions: Due to the small volume required, testing can be completed with a finger prick sample of blood at the general practitioner¡¦s office or at the bedside. The speed, ease-of-use and high analytical performance of the test make it well suited for demanding point-ofcare diagnostic applications.
Background: Small LDL subclasses (sLDL) are known to play a causative role in atherosclerosis and its clinical manifestation-coronary artery disease (CAD). Previous studies show increased prevalence of sLDL in CAD patients with normal levels of traditional lipid risk factors. Carotid intima media thickness (IMT) is considered as a marker of atherosclerosis and in prediction clinical coronary events. Aim: With increasing interest in the role of non-traditional lipid risk factors in CAD, we undertook this study to relate LDL subclass profile and carotid intima-media thickness in CAD patients. Methods: LDL subclasses were separated with 3-31% PAG Electrophoresis, and IMT was determined using high-resolution B-mode ultrasound in 59 patients (age 40-69; 29 females and 30 males) with CAD, with normal levels of traditional lipid risk factors. Results: Mean value of left and right carotid artery measurement was selected as value for correlation with LDL subclass size in each patient. The mean LDL size was 24.97 ± 1.07 nm, and the mean IMT in all patients was 0,89 ± 0,13 mm (0,6-1,2 mm). LDL size was inversely correlated with IMT (r=0,36; p<0,01). Prevalence of subjects with increased IMT was higher among subjects with small LDL subclasses (p<25,5 nm.) Conclusions: LDL size shows a strong association with carotid IMT in CAD patients with normal levels of traditional lipid risk factors and may play an important role in assessment of coronary risk in addition to traditional coronary risk factors. Funding: none
Funding: none
195
194
ULTRASONOGRAPHIC FEATURES OF THE CAROTID ARTERIES AND RISK FACTORS FOR STROKE IN PATIENTS UNDERGOING OPEN HEART SURGERY M. Menih. University Hospital, Maribor, Slovenia
ASSOCIATION BETWEEN LDL SUBCLASSES AND CAROTID INTIMA-MEDIA THICKNESS IN CORONARY ARTERY DISEASE S. Alabakovska1, D. Labudovic1, K. Tosheska1, S. Jovanova2, M. Alabakovski3, B. Todorova1. 1Medical Faculty, Department of Medical Biochemistry, Skopje, Makedonija, 2Medical Faculty, Cardiology Clinic, Skopje, Makedonija, 3Medical School, Skopje, Makedonija
Objective: The aims of this study were to determine the prevalence of carotid artery disease in patients undergoing coronary artery bypass grafting (CABG) and valve replacement (VR) and the association of risk factors with the severity of carotid artery disease and with the coronary artery disease. Methods: We analysed the clinical and laboratory data of 93 patients undergoing VR or/
S90
Research, Eindhoven, The Netherlands, 2Future Diagnostics, Wijchen, The Netherlands Objective: The goal of the present work is to develop a compact handheld device for the rapid and sensitive detection of biomarkers relevant to progression of atherosclerosis and risk stratification of Coronary Artery Disease patients in a point-of-care setting. Methods: The diagnostic test is based on a novel analytical technique that combines active magnetic particles, actuating magnets and a magnetic particle sensor. The complete system consists of a small, disposable cartridge containing 500 nm superparamagnetic particles and a handheld analyzer. A troponin test is reported as an example. Upon the addition of the sample, troponin binds to its antibodies conjugated to the magnetic particles. Actuating electromagnets move the particles through the sample for effective analyte capture. By applying magnetic forces, the particles are then transported with high speed to the sensor surface, which contains capture anti-troponin antibodies. After the particles are allowed to react with the sensor surface, non-specifically bound particles are quickly removed by applying a magnetic force oriented away from the sensor surface. Results: Tests can be performed with a turn-around time of 5 minutes in a sample volume of 25 μL with picomolar sensitivity. Conclusions: Due to the small volume required, testing can be completed with a finger prick sample of blood at the general practitioner¡¦s office or at the bedside. The speed, ease-of-use and high analytical performance of the test make it well suited for demanding point-ofcare diagnostic applications.
Background: Small LDL subclasses (sLDL) are known to play a causative role in atherosclerosis and its clinical manifestation-coronary artery disease (CAD). Previous studies show increased prevalence of sLDL in CAD patients with normal levels of traditional lipid risk factors. Carotid intima media thickness (IMT) is considered as a marker of atherosclerosis and in prediction clinical coronary events. Aim: With increasing interest in the role of non-traditional lipid risk factors in CAD, we undertook this study to relate LDL subclass profile and carotid intima-media thickness in CAD patients. Methods: LDL subclasses were separated with 3-31% PAG Electrophoresis, and IMT was determined using high-resolution B-mode ultrasound in 59 patients (age 40-69; 29 females and 30 males) with CAD, with normal levels of traditional lipid risk factors. Results: Mean value of left and right carotid artery measurement was selected as value for correlation with LDL subclass size in each patient. The mean LDL size was 24.97 ± 1.07 nm, and the mean IMT in all patients was 0,89 ± 0,13 mm (0,6-1,2 mm). LDL size was inversely correlated with IMT (r=0,36; p<0,01). Prevalence of subjects with increased IMT was higher among subjects with small LDL subclasses (p<25,5 nm.) Conclusions: LDL size shows a strong association with carotid IMT in CAD patients with normal levels of traditional lipid risk factors and may play an important role in assessment of coronary risk in addition to traditional coronary risk factors. Funding: none
Funding: none
195
194
ULTRASONOGRAPHIC FEATURES OF THE CAROTID ARTERIES AND RISK FACTORS FOR STROKE IN PATIENTS UNDERGOING OPEN HEART SURGERY M. Menih. University Hospital, Maribor, Slovenia
ASSOCIATION BETWEEN LDL SUBCLASSES AND CAROTID INTIMA-MEDIA THICKNESS IN CORONARY ARTERY DISEASE S. Alabakovska1, D. Labudovic1, K. Tosheska1, S. Jovanova2, M. Alabakovski3, B. Todorova1. 1Medical Faculty, Department of Medical Biochemistry, Skopje, Makedonija, 2Medical Faculty, Cardiology Clinic, Skopje, Makedonija, 3Medical School, Skopje, Makedonija
Objective: The aims of this study were to determine the prevalence of carotid artery disease in patients undergoing coronary artery bypass grafting (CABG) and valve replacement (VR) and the association of risk factors with the severity of carotid artery disease and with the coronary artery disease. Methods: We analysed the clinical and laboratory data of 93 patients undergoing VR or/
S90