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21. Characterization of rosette forming cells in Carp periphral leucocytes
Abstracts
cytes were in the inner portion. Macrophages were observed among the lymphocytes. By 40 days after hatching, thymus differentiated into outer and inner zones: cortex and medulla. At this time blood vessels invaded from the surrounding connective tissue into the thymus. Lymphocytes were also observed in the connective tissue around thymus. From these results the basic structure of the thymus was formed by 40 days after hatching.
VII
phocyte marker in humans and most mammals. We investigated the characterization of carp peripheral leucocytes that form spontaneous rosette with red blood cells of sheep (SRBC), horse (HRBC), bovine (ORBC), and rabbit (RRBC). The frequency of E-rosette formation with SRBC and HRBC was higher than that of ORBC and RRBC. E-rosette formation of carp leucocytes depended on the incubation time and occurred at a temperature ranging from 0"C to 37°C. Variations in surface morphology of re20. ANTIGEN RECEPTOR ON LYMPHO- sere forming cells were observed under the scanCYTES AND MACROPHAGES IN YEL- ning electron microscope as for mammalian lymLOWTAIL, SERIOI~ QUINQU~tADIAT~ phocytes. Rosette forming ability of nylon fiber Masaml Hamaguchl and Rllchi Kusuda*. adhered cells was stronger than that of nylon fiber Nansei National Fisheries Research Instiaae, non-adhered cells. The presence of surface imOno-cho, Hiroshima 739-04, Japan and *Fish munoglobulin among carp peripheral leucocytes DiseaseLaboratory,FacultyofAgriculture,Kachi that forms E-rosettes was proved by the imUniversity,Nankoku, Kochi 783,Japan. Effectof munofluorescence technique, although rosette various antibodies on erythrocyte rosette forma- formation was not dependent on surface Ig. tion by peripheral lymphocytes and peritoneal These results suggest that spontaneous E-rosette maorolShages in unsensitized yeliowtail was inves- formation could not be applicable as a routine tigated. Rabbit red cell (RRBC) and sheep red technique for the classification of carp leublood cell (SRBC) were used. RRBCs were pre- cocytes, unlike mammalian lymphocytes. treated with yeUowtail serum and SRBCs were pre-treated with neuraminidase. Monoclonai 22. CHARACTERISTICS OF CELLS INFILantibodies (Mab) prepared and examined for the TRATING INTO CHV-INDUCED CARP rosette formation-inhibition test were anti-thy- PAPILLOMA. Natsumi Morita and Tokuo mocyte cell surface antigen, anti-macrophage cell Sane. Laboratory of Aquatic Pathology, Tokyo surface antigen, anti-yellowtail Ig and anti-yel- University of Fisheri~, Minato-ku, Tokyo 108, Iowtail complement C3. Anti-lymphocyte and Japan. Carp herpes,Arus (CHV) induces papilanti-macrophage polyclonal antibodies were also loma on carp (Cypfinus carp/o L.), and PBL may made and examined. RRBC rosette formation play an important role in regression. The regreswas inhibited more than 90% by treatment with sion period of CHV-induced tumors appeared in anti-complement C3 Mab, but less than 20% by carp injected with anti-carp PBL serum. In this anti-Ig Mab, suggesting that receptors for com- study, we attempted to characterize cells that plement C3 and Ig are important factors for infiltrate into tumors. Upon uitrastructural obbinding RRBCwithyellowtailcells. On the other servation, two types of granulocytes were recoghand, SRBC rosette formation was inhibited by nized in the papillomatous tissues. One type of pretreatment of lymphocytes and macrophages granulocyte possesses numerous spherical and with anti-Ig Mab. Anti-lymphocyte and anti- high electron-danse granules, and in the other, macrophage Mabs showed no significant inhib- the granules vary in shape with irregular density. itory effect. This indicates that antigen-receptor, Cells harvested from the papillomatous tissue which may be Ig receptor itself or Ig receptor-like were fractionated by percoll gradient density cenmolecule, plays a major role in the binding be- trifugation. In the density of 1.075-1.121g/ tween SRBC and yellowtail cells. ml(F.3) and higher than 1.121g/mi as F.4, granulocyte-like cells were predominant. Granulo21. CHARACTERIZATION OF ROSETTE cyte-like cells in both F3 and F.4 revealed a FORMING CELLS IN CARP PERIPHRAL round shape with eccentric nuclei and were negaLEUCOCYTES. Tadatoshl Kitao and tive for either peroxidase or alkaline Terutoyo Yoshida. Department of Fisheries, phosphatase, but positive for acid phosphatase. Faculty of Agriculture, Miyaz_~ University, Eosinophilic granules were also found in cells of Miyazaki889-21,Jatmtt Erythrocyte (E)-roset te F.3, but granules in cells of F.4 were equivocal. formation has been widely accepted as a T lyre- However, these cells were considered to be sirni-
cytes were in the inner portion. Macrophages were observed among the lymphocytes. By 40 days after hatching, thymus differentiated into outer and inner zones: cortex and medulla. At this time blood vessels invaded from the surrounding connective tissue into the thymus. Lymphocytes were also observed in the connective tissue around thymus. From these results the basic structure of the thymus was formed by 40 days after hatching.
VII
phocyte marker in humans and most mammals. We investigated the characterization of carp peripheral leucocytes that form spontaneous rosette with red blood cells of sheep (SRBC), horse (HRBC), bovine (ORBC), and rabbit (RRBC). The frequency of E-rosette formation with SRBC and HRBC was higher than that of ORBC and RRBC. E-rosette formation of carp leucocytes depended on the incubation time and occurred at a temperature ranging from 0"C to 37°C. Variations in surface morphology of re20. ANTIGEN RECEPTOR ON LYMPHO- sere forming cells were observed under the scanCYTES AND MACROPHAGES IN YEL- ning electron microscope as for mammalian lymLOWTAIL, SERIOI~ QUINQU~tADIAT~ phocytes. Rosette forming ability of nylon fiber Masaml Hamaguchl and Rllchi Kusuda*. adhered cells was stronger than that of nylon fiber Nansei National Fisheries Research Instiaae, non-adhered cells. The presence of surface imOno-cho, Hiroshima 739-04, Japan and *Fish munoglobulin among carp peripheral leucocytes DiseaseLaboratory,FacultyofAgriculture,Kachi that forms E-rosettes was proved by the imUniversity,Nankoku, Kochi 783,Japan. Effectof munofluorescence technique, although rosette various antibodies on erythrocyte rosette forma- formation was not dependent on surface Ig. tion by peripheral lymphocytes and peritoneal These results suggest that spontaneous E-rosette maorolShages in unsensitized yeliowtail was inves- formation could not be applicable as a routine tigated. Rabbit red cell (RRBC) and sheep red technique for the classification of carp leublood cell (SRBC) were used. RRBCs were pre- cocytes, unlike mammalian lymphocytes. treated with yeUowtail serum and SRBCs were pre-treated with neuraminidase. Monoclonai 22. CHARACTERISTICS OF CELLS INFILantibodies (Mab) prepared and examined for the TRATING INTO CHV-INDUCED CARP rosette formation-inhibition test were anti-thy- PAPILLOMA. Natsumi Morita and Tokuo mocyte cell surface antigen, anti-macrophage cell Sane. Laboratory of Aquatic Pathology, Tokyo surface antigen, anti-yellowtail Ig and anti-yel- University of Fisheri~, Minato-ku, Tokyo 108, Iowtail complement C3. Anti-lymphocyte and Japan. Carp herpes,Arus (CHV) induces papilanti-macrophage polyclonal antibodies were also loma on carp (Cypfinus carp/o L.), and PBL may made and examined. RRBC rosette formation play an important role in regression. The regreswas inhibited more than 90% by treatment with sion period of CHV-induced tumors appeared in anti-complement C3 Mab, but less than 20% by carp injected with anti-carp PBL serum. In this anti-Ig Mab, suggesting that receptors for com- study, we attempted to characterize cells that plement C3 and Ig are important factors for infiltrate into tumors. Upon uitrastructural obbinding RRBCwithyellowtailcells. On the other servation, two types of granulocytes were recoghand, SRBC rosette formation was inhibited by nized in the papillomatous tissues. One type of pretreatment of lymphocytes and macrophages granulocyte possesses numerous spherical and with anti-Ig Mab. Anti-lymphocyte and anti- high electron-danse granules, and in the other, macrophage Mabs showed no significant inhib- the granules vary in shape with irregular density. itory effect. This indicates that antigen-receptor, Cells harvested from the papillomatous tissue which may be Ig receptor itself or Ig receptor-like were fractionated by percoll gradient density cenmolecule, plays a major role in the binding be- trifugation. In the density of 1.075-1.121g/ tween SRBC and yellowtail cells. ml(F.3) and higher than 1.121g/mi as F.4, granulocyte-like cells were predominant. Granulo21. CHARACTERIZATION OF ROSETTE cyte-like cells in both F3 and F.4 revealed a FORMING CELLS IN CARP PERIPHRAL round shape with eccentric nuclei and were negaLEUCOCYTES. Tadatoshl Kitao and tive for either peroxidase or alkaline Terutoyo Yoshida. Department of Fisheries, phosphatase, but positive for acid phosphatase. Faculty of Agriculture, Miyaz_~ University, Eosinophilic granules were also found in cells of Miyazaki889-21,Jatmtt Erythrocyte (E)-roset te F.3, but granules in cells of F.4 were equivocal. formation has been widely accepted as a T lyre- However, these cells were considered to be sirni-