- Email: [email protected]
4430318 Immunoassay utilizing 1251 protein a
xi
New Patents
4430258 METHOD OF PRODUCING LIQUID EQUIVALENT SOLID GAMMA RAY CALIBRATION STANDARDS Robert McFarland.
Patricia A McFarland
Method of producing liquid equivalent solid gamma ray calibration standards comprising the steps of adding a first organic solvent to a calibrated aqueous solution of radioactive material, either in the form of a single isotope or mixed isotopes. to form a first solution; mixing the first solution with a polymerizible resin dissolved in a second organic solvent together with a hardening catalyst to form a second solution; and curing the second solution. With isotope standards requiring highly acidic conditions, a stabilizing agent is added to the first solvent to complex with metal and hydrogen ions so as to prevent plate-out.
4430318 IMMUNOASSAY UTILIZING PROTEIN A
1251
John Langone assigned to The United States of America as represented by the Department of Health and Human Services
An improved method for the preparation of 125I-labelled Protein A (1251 PA) of high specific and functional activity. 1251 PA has been used in combination with purified rabbit IgG (immunoglobulin G) bound to a solid support to develop a competitive binding assay capable of detecting Protein A or human, rabbit and guinea pig IgG at the nanogram level. Additionally. 1251 PA may be used to detect methotrexate. leucovorin and similar substances. 1351 PA has also been used to detect IgG anti-Forssman antibody bound to sheep erythrocytes and to line- 1 and line- 10 tumor cells and as an indirect assay for tumor associated antigen in the ascitic fluid of tumor-bearing
guinea pigs. Additionally, an improved method of preparation of iodination of Protein A is . utilized. This procedure used the Bolton-Hunter (1973) reagent of radioactive iodine in benzene which carrier is evaporated. The PA is added or attached while in an amino acid mixture and separation is per column utilizing Sephadex G25 and VBS-gel.
4430319 RADIOACTIVE IODINE LABELED PHENOLIC AMINES Monte Blau, Hank F Kung, Kenneth M Tramposch assigned to State University of New York OH
The invention is accomplished by the formation at the site of use of the radioactive amine of the invention which is then injected immediately into the mamma1 for diagnostic purposes. The compounds of the invention are as follows: See Patent _for Chemical Structure In the above compound, Rl, R2. R3 and R4 are independently any straight or branched alkyl of between 1 and about 10 carbons; n may be between 1 and about 20. In the invention, the radio labeling of the invention compound is accomplished immediately prior to use of the radio labeled material. The compounds of the invention when boiled for about 15 minutes with iodine- 123, a radioactive material, undergo a substitution of radioactive for non-radioactive iodine such that the compound becomes labeled and suitable for use. The compound itself, prior to radio labeling, is storage stable.
4430320 RADIOACTIVE DIAGNOSTIC AGENT Akiy Shigematsu, Akira Tsuya, Michiaki Aihara, Akiko. Suzuki, Michiko Matsuda, Hachioji, Japan assigned to Kabushiki Kaisha Seitai Kagaku Kenkyusho A radioactive diagnostic agent for renal cortex, adrenal cortex. myocardium, brain stem, spinal nerve. etc., which comprises as an essential component monoiodoacetic acid wherein the iodine atom is radioactive.
New Patents
4430258 METHOD OF PRODUCING LIQUID EQUIVALENT SOLID GAMMA RAY CALIBRATION STANDARDS Robert McFarland.
Patricia A McFarland
Method of producing liquid equivalent solid gamma ray calibration standards comprising the steps of adding a first organic solvent to a calibrated aqueous solution of radioactive material, either in the form of a single isotope or mixed isotopes. to form a first solution; mixing the first solution with a polymerizible resin dissolved in a second organic solvent together with a hardening catalyst to form a second solution; and curing the second solution. With isotope standards requiring highly acidic conditions, a stabilizing agent is added to the first solvent to complex with metal and hydrogen ions so as to prevent plate-out.
4430318 IMMUNOASSAY UTILIZING PROTEIN A
1251
John Langone assigned to The United States of America as represented by the Department of Health and Human Services
An improved method for the preparation of 125I-labelled Protein A (1251 PA) of high specific and functional activity. 1251 PA has been used in combination with purified rabbit IgG (immunoglobulin G) bound to a solid support to develop a competitive binding assay capable of detecting Protein A or human, rabbit and guinea pig IgG at the nanogram level. Additionally. 1251 PA may be used to detect methotrexate. leucovorin and similar substances. 1351 PA has also been used to detect IgG anti-Forssman antibody bound to sheep erythrocytes and to line- 1 and line- 10 tumor cells and as an indirect assay for tumor associated antigen in the ascitic fluid of tumor-bearing
guinea pigs. Additionally, an improved method of preparation of iodination of Protein A is . utilized. This procedure used the Bolton-Hunter (1973) reagent of radioactive iodine in benzene which carrier is evaporated. The PA is added or attached while in an amino acid mixture and separation is per column utilizing Sephadex G25 and VBS-gel.
4430319 RADIOACTIVE IODINE LABELED PHENOLIC AMINES Monte Blau, Hank F Kung, Kenneth M Tramposch assigned to State University of New York OH
The invention is accomplished by the formation at the site of use of the radioactive amine of the invention which is then injected immediately into the mamma1 for diagnostic purposes. The compounds of the invention are as follows: See Patent _for Chemical Structure In the above compound, Rl, R2. R3 and R4 are independently any straight or branched alkyl of between 1 and about 10 carbons; n may be between 1 and about 20. In the invention, the radio labeling of the invention compound is accomplished immediately prior to use of the radio labeled material. The compounds of the invention when boiled for about 15 minutes with iodine- 123, a radioactive material, undergo a substitution of radioactive for non-radioactive iodine such that the compound becomes labeled and suitable for use. The compound itself, prior to radio labeling, is storage stable.
4430320 RADIOACTIVE DIAGNOSTIC AGENT Akiy Shigematsu, Akira Tsuya, Michiaki Aihara, Akiko. Suzuki, Michiko Matsuda, Hachioji, Japan assigned to Kabushiki Kaisha Seitai Kagaku Kenkyusho A radioactive diagnostic agent for renal cortex, adrenal cortex. myocardium, brain stem, spinal nerve. etc., which comprises as an essential component monoiodoacetic acid wherein the iodine atom is radioactive.