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C-reactive protein immunoassay
Advances
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rate and serum fibrinogen concentration. Serum C-reactive protein is an acute phase marker in dogs that is also useful for monitoring of inflammatory diseases. For Creactive protein to be of use in clinical situations, a rapid, reliable, and automated assay is needed. Objectives To determine if canine serum C-reactive protein concentration could be measured reliably using an automated turbidimetric immunoassay (TIA) designed for use with human serum. Procedure A commercially available TIA for human serum C-reactive protein was validated in dogs and then used to measure canine serum C-reactive protein concentration in healthy dogs and dogs with inflammatory diseases. The assay was applied to serum samples from 14 clinically healthy dogs, 11 with neoplasia, 13 with infections, 8 with endocrine or metabolic diseases, and 10 with miscellaneous diseases. Results The antibody for C-reactive protein used in the assay to detect human C-reactive protein was found to detect canine Creactive protein with acceptable precision. Dogs with infections had a significantly higher concentration of serum C-reactive protein than did all other dogs. Dogs with neoplasia had a significantly higher concentration of serum C-reactive protein than did clinically healthy dogs. Author Conclusion Canine serum C-reactive protein concentration can be measured reliably using the commercially available TIA designed for human C-reactive protein. Inclusions Four figures, 3 tables, 37 references.
Clinical Pathology C-Reactive Protein Immunoassay Background Laboratory tests to monitor inflammation have included erythrocyte sedimentation
Editor Annotation Serum C-reactive protein is an acutephase reactant which is not influenced by red cell count or hyperglobulinemia as is the erythrocyte sedimentation rate or calculated serum fibrinogen concentration. In people, it is most useful in monitoring immune-mediated disease. C-reactive protein increases within 4 to 6 hours of acute inflammation, and the quantity measured
Advances reflects the intensity of inflammation. It decreases or disappears rapidly when inflammation is appropriately treated or successfully ameliorated with glucocorticoids or nonsteroidal anti-inflammatory drugs. If it does not decrease after an injury, tissue damage is considered ongoing. The highest concentrations are found in bacterial infections and can be used to monitor disease activity with infectious processes. It is also useful in monitoring neoplastic activity. This human quantitative C-reactive protein automated TIA appears to be both reliable and useful in dogs and cats. It certainly differentiates between healthy and abnormal in the majority of clinical situations. (BFF) Kjelgaard-Hansen M, Jensen AL, Kristensen AT. Evaluation of a commercially available human C-reactive protein (CRP) turbidimetric immunoassay for determination of canine serum CRP concentration. Vet Clin Pathol 2003;32:81-87.
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rate and serum fibrinogen concentration. Serum C-reactive protein is an acute phase marker in dogs that is also useful for monitoring of inflammatory diseases. For Creactive protein to be of use in clinical situations, a rapid, reliable, and automated assay is needed. Objectives To determine if canine serum C-reactive protein concentration could be measured reliably using an automated turbidimetric immunoassay (TIA) designed for use with human serum. Procedure A commercially available TIA for human serum C-reactive protein was validated in dogs and then used to measure canine serum C-reactive protein concentration in healthy dogs and dogs with inflammatory diseases. The assay was applied to serum samples from 14 clinically healthy dogs, 11 with neoplasia, 13 with infections, 8 with endocrine or metabolic diseases, and 10 with miscellaneous diseases. Results The antibody for C-reactive protein used in the assay to detect human C-reactive protein was found to detect canine Creactive protein with acceptable precision. Dogs with infections had a significantly higher concentration of serum C-reactive protein than did all other dogs. Dogs with neoplasia had a significantly higher concentration of serum C-reactive protein than did clinically healthy dogs. Author Conclusion Canine serum C-reactive protein concentration can be measured reliably using the commercially available TIA designed for human C-reactive protein. Inclusions Four figures, 3 tables, 37 references.
Clinical Pathology C-Reactive Protein Immunoassay Background Laboratory tests to monitor inflammation have included erythrocyte sedimentation
Editor Annotation Serum C-reactive protein is an acutephase reactant which is not influenced by red cell count or hyperglobulinemia as is the erythrocyte sedimentation rate or calculated serum fibrinogen concentration. In people, it is most useful in monitoring immune-mediated disease. C-reactive protein increases within 4 to 6 hours of acute inflammation, and the quantity measured
Advances reflects the intensity of inflammation. It decreases or disappears rapidly when inflammation is appropriately treated or successfully ameliorated with glucocorticoids or nonsteroidal anti-inflammatory drugs. If it does not decrease after an injury, tissue damage is considered ongoing. The highest concentrations are found in bacterial infections and can be used to monitor disease activity with infectious processes. It is also useful in monitoring neoplastic activity. This human quantitative C-reactive protein automated TIA appears to be both reliable and useful in dogs and cats. It certainly differentiates between healthy and abnormal in the majority of clinical situations. (BFF) Kjelgaard-Hansen M, Jensen AL, Kristensen AT. Evaluation of a commercially available human C-reactive protein (CRP) turbidimetric immunoassay for determination of canine serum CRP concentration. Vet Clin Pathol 2003;32:81-87.
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