4526782 Process for recovering interferon

PATENT ABSTRACTS

permeability of 10, 000 to 50,000 I) in circulation against water, whereby conccntralion is conducted before a n d o r after diaiittration.

4526782 PROCESS FOR RECOVERING INTERFERON Yahiro Uemura, Hirofum Arimura, Itiroshi Morise, Satoshi Funakoshi, Tadakazu Suyama, Hirakata, Japan assigned to The Green Cross Corporation Interferon can be recovered simply by contacting an aqueous solution cuntaming interferon which is induced and produced from cells of human origin capable of producing interferon, with a siliceous substance to adsorb interferon on it and eluting the adsorbed interferon with an aqueous solution containing a nonionic surfactant, preferably further containing an acriflavine such as acrinol.

45~!~ METHOD OF SEPARATING AI4-125I-INSULIN FROM HETEROGENEOUSLY LABELED INSULIN MOLECULES FOR BIOLOGICAL STUDIES Frankie B Stentz, Reba K Wright, Abbas E Kitabchi assigned to University of TN Research Corporation ~

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cl~_uE~i~SEp~ATtON OF"U ~ I , ~ D INSIUN

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Method of separating A14-125I-insulin from heterogeneously labeled insulin molecules for biological studies which involves iodating insulin in a controlled manner, selectively absorbing the insulin from the iodination procedure in a tightly packed bed of octadecylsilane bonded to silica employing reverse phase liquid chromatography principles, thereafter eluting large and small radioactive and nonradioactive materials such as damaged insulin, various contaminants and polar materials from the bed with

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dilute trilluoroacetic acid, thereafter eluting the inlact 1251-insulin from thc bed with a mixture of acctonitrile and dilute t rifluoroacetic acid and thcn separating the Al4-insulin by high perlbrmancc liquid chromatography or ion exchange chromatography.

4528186 METHOD OF PRODUCING HUMAN EPIDERMAL GROWTH FACTOR Toyohiko Nishimura, Naomi Uchida, Hajime Hiralani, Ashiya. Japan assigned to Japan Chemical Rcsearch Co Ltd A method for producing a human epidermal growth factor in high yield and high purity by adding particles of an aluminum or magnesium silicate of the formula z.2 (or 6) SiO2.xH20 whcre Z is AI203 or 2MgO and x is the number of water molecules in each molecule of the compound, to human urine under neutral to acid conditions to adsorb the factor on the particles and then eluting the factor with an alkaline solution.

45~1~ POLYSACCHARIDE PS-A OBTAINED FROM BARRENWORT DERIVING FROM PLANTS BELONGING TO THE GENUS EPIMEDIUM, PROCESS FOR PREPARATION THEREOF AND PHYLACTIC AND IMMUNOSTIMULATING AGENTS COMPRISING SAID POLYSACCHARIDE PS-A EFFECTIVE COMPONENT Susumu Mitsuhashi, Muneaki Takase, Sosuke Yasui, Ichiro Wa~hizawa, Kimitomo Yoshioka, Tokyo, Japan assigned to Zenyaku Kogyo Kabushiki Kaisha A polysaccharide PS-A obtained from plants belonging to the genus Epimedium and having the following physicochemical properties, a process for preparation thereof and use as phylactic and immunostimulating agents comprising the polysaccharide PS-A as an effective component: I. Elementary analysis: C=40.92; H=6.17; Ash = very small; 2. Molecular weight: 75,000 +/-25,000 (average molecular weight); 3. Decomposition point: 205 degrees C.; 4. pH: 7.0 (solution of 100 mg of PS-A in 50 ml of water); 5. Specific rotatory power: ( alpha)DI9=-23.6 degrees (in H20, c = 0.527); 6. Infrared absorption spectrum: numaxKBr(cm-l)/3400, 2900, 1620, 1400, 1230, 1060; 7. Ultraviolet absorption