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4616079 Immunoamplifiers and processes for the extraction thereof
181
PATENT ABSTRACTS
4615974 YEAST EXPRESSION
VECTORS
Alan Kingsman, Susan M Kingsman. Islip, United Kingdom assigned to Celltech Limited There are described a number of plasmid vectors suitable for the expression of genetic material, at various levels in yeasts. The plasmids each comprise a yeast selective marker, a yeast replication origin and a yeast promoter positioned relative to a unique restriction site in such a way that expression may be obtained of a polypeptide coding sequence inserted at the restriction site. The promoters used are derived from the 5' region of a gene coding for a yeast glycolytic enzyme e.g. phosphoglycerate kinase (PGK), or from the 5" region of the yeast TRPI gene. In one Example a plasmid contains a promoter derived from both the 3" and 5' regions of the PGK gene. The replication systems used involve the yeast 2 mu replication origin or an autonomous replicating sequence (ARS) stabilized with an ARS stabilizing sequence (ASS). The replication systems allow for a choice of high or low copy number per cell. The promoter sequences allow for a choice of high or low expression level. A kit including vectors having a combination of these alternative features is described. Yeast expression vectors including a gene for coding for human interferon- alpha are described.
4616079 IMMUNOAMPLIFIERS AND PROCESSES FOR THE EXTRACTION THEREOF A Arthur Gottlieb assigned to lmreg Inc A high pressure liquid chromatography process is described for the extraction of materials that nonspecifically amplify the immune response to antigens to which the subject was previously or is concomitantly exposed. The materials are also described, as are methods and compositions for the use thereof in connection with hypoimmune conditions.
solution of an active ingredient to be encapsulated and a film-forming polymer, removing the solvent to provide a dry, composite, uniform admixture of the active ingredient and the polymer material, reducing the composite admixture to a defined smaller particle size distribution. coating the reduced composite core particles in a fluidized bed. with a uniform defined wall thickness of the same or substantially the same filmforming polymer material to provide composite core coated microparticles. 4623621 IMMUNOASSAY AND PROTEIN
FOR PEPTIDE OLIGOMERS
Sidney Pestka assigned to Hoffmann-La Roche Inc A convenient immunoassay for detecting the presence of oligomeric forms of peptides and proteins is described. The assay employs the sandwich technique with a monoclonal antibody selective for a single epitope on the peptide or protein bound to a solid phase and the same monoclonal antibody labeled with a detectable label in the solution phase. 4624846 METHOD FOR ENHANCING TARGET SPECIFICITY OF ANTIBODY LOCALIZATION AND CLEARANCE OF NON-TARGET DIAGNOSTIC AND THERAPEUTIC PRINCIPLES Milton D Goldenberg assigned to Immunomedics Inc A method for enhancing target specificity of antibody localization comprises injecting a second antibody specific to a labeled targetspecific antibody to reduce the level of nontargeted circulating specific antibody, thereby increasing the localization ratio. The foregoing method is useful for imaging tumors and infectious lesions, and for therapy.
4623588 4624918 CONTROLLED RELEASE COMPOSITE CORE COATED MICROPARTICLES
PURIFICATION PROCESS FOR HEPATITIS SURFACE ANTIGEN AND PRODUCT THEREOF
Elie S Nuwayser, William A Nucefora assigned to Biotek lnc
Robert Hershberg assigned to Genentech Inc
A process for preparing coated microparticles, which process comprises preparing a solvent
A method for the purification of hepatitis B surface antigen from recombinant cell culture tom-
PATENT ABSTRACTS
4615974 YEAST EXPRESSION
VECTORS
Alan Kingsman, Susan M Kingsman. Islip, United Kingdom assigned to Celltech Limited There are described a number of plasmid vectors suitable for the expression of genetic material, at various levels in yeasts. The plasmids each comprise a yeast selective marker, a yeast replication origin and a yeast promoter positioned relative to a unique restriction site in such a way that expression may be obtained of a polypeptide coding sequence inserted at the restriction site. The promoters used are derived from the 5' region of a gene coding for a yeast glycolytic enzyme e.g. phosphoglycerate kinase (PGK), or from the 5" region of the yeast TRPI gene. In one Example a plasmid contains a promoter derived from both the 3" and 5' regions of the PGK gene. The replication systems used involve the yeast 2 mu replication origin or an autonomous replicating sequence (ARS) stabilized with an ARS stabilizing sequence (ASS). The replication systems allow for a choice of high or low copy number per cell. The promoter sequences allow for a choice of high or low expression level. A kit including vectors having a combination of these alternative features is described. Yeast expression vectors including a gene for coding for human interferon- alpha are described.
4616079 IMMUNOAMPLIFIERS AND PROCESSES FOR THE EXTRACTION THEREOF A Arthur Gottlieb assigned to lmreg Inc A high pressure liquid chromatography process is described for the extraction of materials that nonspecifically amplify the immune response to antigens to which the subject was previously or is concomitantly exposed. The materials are also described, as are methods and compositions for the use thereof in connection with hypoimmune conditions.
solution of an active ingredient to be encapsulated and a film-forming polymer, removing the solvent to provide a dry, composite, uniform admixture of the active ingredient and the polymer material, reducing the composite admixture to a defined smaller particle size distribution. coating the reduced composite core particles in a fluidized bed. with a uniform defined wall thickness of the same or substantially the same filmforming polymer material to provide composite core coated microparticles. 4623621 IMMUNOASSAY AND PROTEIN
FOR PEPTIDE OLIGOMERS
Sidney Pestka assigned to Hoffmann-La Roche Inc A convenient immunoassay for detecting the presence of oligomeric forms of peptides and proteins is described. The assay employs the sandwich technique with a monoclonal antibody selective for a single epitope on the peptide or protein bound to a solid phase and the same monoclonal antibody labeled with a detectable label in the solution phase. 4624846 METHOD FOR ENHANCING TARGET SPECIFICITY OF ANTIBODY LOCALIZATION AND CLEARANCE OF NON-TARGET DIAGNOSTIC AND THERAPEUTIC PRINCIPLES Milton D Goldenberg assigned to Immunomedics Inc A method for enhancing target specificity of antibody localization comprises injecting a second antibody specific to a labeled targetspecific antibody to reduce the level of nontargeted circulating specific antibody, thereby increasing the localization ratio. The foregoing method is useful for imaging tumors and infectious lesions, and for therapy.
4623588 4624918 CONTROLLED RELEASE COMPOSITE CORE COATED MICROPARTICLES
PURIFICATION PROCESS FOR HEPATITIS SURFACE ANTIGEN AND PRODUCT THEREOF
Elie S Nuwayser, William A Nucefora assigned to Biotek lnc
Robert Hershberg assigned to Genentech Inc
A process for preparing coated microparticles, which process comprises preparing a solvent
A method for the purification of hepatitis B surface antigen from recombinant cell culture tom-