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4622293 Iodothyronine immunoassays employing HMS as TBP blocking agent
155
PATENT ABSTRACTS
4621049 ENZYMATIC HIGH RANGE GLUCOSE TEST Joseph Y Wang assigned to Miles Laboratories Inc A test composition comprising glucose oxidase, a peroxidatively active component, a chromogenic indicator system capable of providing a detectable response and a borate buffer capable of providing an initial pH above about pH 7 is particularly useful for the semiquantitative determination of high range glucose, (i.e. glucose concentrations of l, 000 mg/dL to 10,000 mg/dL). A preferred indicator system is a water soluble iodide salt and poly(vinylpyrrolidone). The use of a borate buffer capable of providing an initial pH above about pH 7 permits greatly improved resolution for the semiquantitative determination of high range glucose when the test composition is incorported onto a carrier matrix to prepare a solid state unitary test device.
4621059 APPARATUS FOR MEASURING VELOCITY OF ENZYME REACTION Kyuj Rokugawa, Ootawara, Japan assigned to Kabushiki Kaisha Toshiba An enzyme is immobilized to a capillary column, and a solution which contains a chemical substance and a luminescent substance flows through the column. A plurality of optical fibers are arranged along the longitudinal direction of the column, and the luminescence in the column is introduced to a photodiode array through the fibers. The distribution of the luminescent intensity along the longitudinal direction of the column is detected by the photodiode array. Thus, the rate of quantity increase of the product produced by the enzyme reaction can be measured while the solution is flowing through the column, thereby producing the enzyme activity by the end assay and the rate assay.
4622292 ASSAY AND KIT FOR DETERMINING INTERFERON
4621055 PROCESS FOR PRODUCING BIOLOGICALLY ACTIVE FACTORS Karl Theurer, 7302 Ostfildern 1 (Ruit), Federal Republic Of Germany A process for producing, biologically active factors from a substrate, in the form of cell homogenates of organ tissues, of microorganisms, plant components and/or body fluids. To this end, the substrate, in an aqueous form and freed of accompanying particulate substances, is selectively separated by affinity chromatography using a biological sorbent, in the case of which at least one nucleic acid (desoxyribonucleic and/or ribonucleic acid) or at least one protein or peptide is coupled to a carrier substance; in the primary eluate components having no affinity are present, while the active factors (which have an affinity) are secondarily eluated. By binding nucleic acids or proteins from a given origin to a carrier, active factors with special properties such as tumor inhibiting substances, or stimulating substances may be positively produced.
Michel Revel, David Wallach, Rehovot, Israel assigned to Yeda Research and Development Company Ltd An assay and kit for the detection and quantitative determination of interferon. The assay is based on the exposure of certain cells to a solution containing the interferon which is to be determined, infecting the cells with a certain virus, incubating for a predetermined period of time, lysing the infected cultures and determining the virus protein. The measurement of the virus proteins can be effected by ELISA or radioimmunoassay.
4622293 IODOTHYRONINE IMMUNOASSAYS EMPLOYING HMS AS TBP BLOCKING AGENT Paul B Ellis, David Morris assigned to Miles Laboratories lnc An improved immunoassay method, reagent means, test kit, and test device for determining
PATENT ABSTRACTS
156
an iodothyronine, e.g., thyroxine (T-4), in a biological fluid, usually serum or plasma, wherein 2hydroxy-4-methoxybenzophenone-5-sulfonic acid (HMS), or a salt thereof, is employed as a blocking agent for the binding ofiodothyronines to thyroxine binding protein (TBP). The present invention is particularly advantageous as applied to homogeneous competitive binding iodothyronine immunoassays employing labels which are participants in enzyme-catalyzed reactions. Such labels include enzyme substrates, coenzymes, enzyme inhibitors, enzyme prosthetic groups, and enzymes.
4622294 LIPOSOME IMMUNOASSAY REAGENT AND METHOD Viola T Kung, Eleanor Canova-Davis A liposome assay reagent for determination of an analyte in a homogeneous immunoassay. The reagent includes a suspension of oligolamellar lipid vesicles containing encapsulated glucose-6phosphate dehydrogenase (G6PD), at a specific activity of between about 1-10 units/ mumole vesicle lipid, and glucose-6-phosphate (G6P) at a concentration of at least about 5 raM. The encapsulated G6P protects the enzyme against inactivation on preparation, by reverse phase evaporation in the presence of organic solvent, and on storage as an aqueous suspension.
4622296 PROCESS FOR MEASURING ACTIVITY OF DEHYDROGENASE EMPLOYING A REACTION STOPPER Kazuhiko Yamanishi, Toshiro Hanada, Tokyo, Japan assigned to Wako Pure Chemical Industries Ltd In a process for measuring the activity ofa dehydrogenase or the amount of substrate reacted in the presence of the dehydrogenase using NAD or NADP as coenzyme and a tetrazolium salt as a color-producing reagent, when at least one member selected from the group consisting of dodecyl sulfate, decyl sulfate, dodecylbenzenesulfonic acid, and salts thereof is used as a reaction stopper, resulting in enhancement of coloring. stability of the produced color and prevention of the produced f~,rmazan compound from staining the laboratoryware.
4622297 PROCESS AND AGENT FOR TESTING THE SENSITIVITY OF BACTERIA Manfred Kappner, Harald Metz, Reinheim, Federal Republic Of Germany assigned to Merck Patent Gesellschaft mit beschrankter Haftung A process and agent are provided for testing the sensitivity of bacteria towards antibiotics with a primary action in murein biosynthesis. The process is characterized in that the cytoplasmic enzyme activities released from the bacteria in the presence of the antibiotic and an enzyme substrate are determined directly.
4622295 MODIFIED OLIGOSACCHARIDES USED AS SUBSTRATE FOR MEASURING ALPHA-AMYLASE ACTIVITY Tokuji Ikenaka, Kaoru Omichi, Sakai, Japan assigned to Wako Pure Chemical Industries Ltd A modified oligosaccharide having at most 7 glucose units and having a substituent selected from the group consisting of 2-aminopyridyl, 3aminopyridyl, anilino, methylanilino, hydroxyanilino, carboxyphenylamino and hydroxyl groups at at least one end moiety of said oligosaccharide is suitable as substrate for measuring alpha-amylase activity or for measuring alpha-amylase isozymes.
4622298 DETECTION AND QUANTITATION OF MICROORGANISMS, LEUKOCYTES AND SQUAMOUS EPITHELIAL CELLS IN URINE James D Mansour, Thomas H Schulte, Burton H Sage assigned to Becton Dickinson and Company A method for the assessment of bacteriuria and pyuria includes the simultaneous detection and quantitation of microorganisms, leukocytes and squamous epithelial cells in a urine specimen. The three cell types are stained with a fluorescent
PATENT ABSTRACTS
4621049 ENZYMATIC HIGH RANGE GLUCOSE TEST Joseph Y Wang assigned to Miles Laboratories Inc A test composition comprising glucose oxidase, a peroxidatively active component, a chromogenic indicator system capable of providing a detectable response and a borate buffer capable of providing an initial pH above about pH 7 is particularly useful for the semiquantitative determination of high range glucose, (i.e. glucose concentrations of l, 000 mg/dL to 10,000 mg/dL). A preferred indicator system is a water soluble iodide salt and poly(vinylpyrrolidone). The use of a borate buffer capable of providing an initial pH above about pH 7 permits greatly improved resolution for the semiquantitative determination of high range glucose when the test composition is incorported onto a carrier matrix to prepare a solid state unitary test device.
4621059 APPARATUS FOR MEASURING VELOCITY OF ENZYME REACTION Kyuj Rokugawa, Ootawara, Japan assigned to Kabushiki Kaisha Toshiba An enzyme is immobilized to a capillary column, and a solution which contains a chemical substance and a luminescent substance flows through the column. A plurality of optical fibers are arranged along the longitudinal direction of the column, and the luminescence in the column is introduced to a photodiode array through the fibers. The distribution of the luminescent intensity along the longitudinal direction of the column is detected by the photodiode array. Thus, the rate of quantity increase of the product produced by the enzyme reaction can be measured while the solution is flowing through the column, thereby producing the enzyme activity by the end assay and the rate assay.
4622292 ASSAY AND KIT FOR DETERMINING INTERFERON
4621055 PROCESS FOR PRODUCING BIOLOGICALLY ACTIVE FACTORS Karl Theurer, 7302 Ostfildern 1 (Ruit), Federal Republic Of Germany A process for producing, biologically active factors from a substrate, in the form of cell homogenates of organ tissues, of microorganisms, plant components and/or body fluids. To this end, the substrate, in an aqueous form and freed of accompanying particulate substances, is selectively separated by affinity chromatography using a biological sorbent, in the case of which at least one nucleic acid (desoxyribonucleic and/or ribonucleic acid) or at least one protein or peptide is coupled to a carrier substance; in the primary eluate components having no affinity are present, while the active factors (which have an affinity) are secondarily eluated. By binding nucleic acids or proteins from a given origin to a carrier, active factors with special properties such as tumor inhibiting substances, or stimulating substances may be positively produced.
Michel Revel, David Wallach, Rehovot, Israel assigned to Yeda Research and Development Company Ltd An assay and kit for the detection and quantitative determination of interferon. The assay is based on the exposure of certain cells to a solution containing the interferon which is to be determined, infecting the cells with a certain virus, incubating for a predetermined period of time, lysing the infected cultures and determining the virus protein. The measurement of the virus proteins can be effected by ELISA or radioimmunoassay.
4622293 IODOTHYRONINE IMMUNOASSAYS EMPLOYING HMS AS TBP BLOCKING AGENT Paul B Ellis, David Morris assigned to Miles Laboratories lnc An improved immunoassay method, reagent means, test kit, and test device for determining
PATENT ABSTRACTS
156
an iodothyronine, e.g., thyroxine (T-4), in a biological fluid, usually serum or plasma, wherein 2hydroxy-4-methoxybenzophenone-5-sulfonic acid (HMS), or a salt thereof, is employed as a blocking agent for the binding ofiodothyronines to thyroxine binding protein (TBP). The present invention is particularly advantageous as applied to homogeneous competitive binding iodothyronine immunoassays employing labels which are participants in enzyme-catalyzed reactions. Such labels include enzyme substrates, coenzymes, enzyme inhibitors, enzyme prosthetic groups, and enzymes.
4622294 LIPOSOME IMMUNOASSAY REAGENT AND METHOD Viola T Kung, Eleanor Canova-Davis A liposome assay reagent for determination of an analyte in a homogeneous immunoassay. The reagent includes a suspension of oligolamellar lipid vesicles containing encapsulated glucose-6phosphate dehydrogenase (G6PD), at a specific activity of between about 1-10 units/ mumole vesicle lipid, and glucose-6-phosphate (G6P) at a concentration of at least about 5 raM. The encapsulated G6P protects the enzyme against inactivation on preparation, by reverse phase evaporation in the presence of organic solvent, and on storage as an aqueous suspension.
4622296 PROCESS FOR MEASURING ACTIVITY OF DEHYDROGENASE EMPLOYING A REACTION STOPPER Kazuhiko Yamanishi, Toshiro Hanada, Tokyo, Japan assigned to Wako Pure Chemical Industries Ltd In a process for measuring the activity ofa dehydrogenase or the amount of substrate reacted in the presence of the dehydrogenase using NAD or NADP as coenzyme and a tetrazolium salt as a color-producing reagent, when at least one member selected from the group consisting of dodecyl sulfate, decyl sulfate, dodecylbenzenesulfonic acid, and salts thereof is used as a reaction stopper, resulting in enhancement of coloring. stability of the produced color and prevention of the produced f~,rmazan compound from staining the laboratoryware.
4622297 PROCESS AND AGENT FOR TESTING THE SENSITIVITY OF BACTERIA Manfred Kappner, Harald Metz, Reinheim, Federal Republic Of Germany assigned to Merck Patent Gesellschaft mit beschrankter Haftung A process and agent are provided for testing the sensitivity of bacteria towards antibiotics with a primary action in murein biosynthesis. The process is characterized in that the cytoplasmic enzyme activities released from the bacteria in the presence of the antibiotic and an enzyme substrate are determined directly.
4622295 MODIFIED OLIGOSACCHARIDES USED AS SUBSTRATE FOR MEASURING ALPHA-AMYLASE ACTIVITY Tokuji Ikenaka, Kaoru Omichi, Sakai, Japan assigned to Wako Pure Chemical Industries Ltd A modified oligosaccharide having at most 7 glucose units and having a substituent selected from the group consisting of 2-aminopyridyl, 3aminopyridyl, anilino, methylanilino, hydroxyanilino, carboxyphenylamino and hydroxyl groups at at least one end moiety of said oligosaccharide is suitable as substrate for measuring alpha-amylase activity or for measuring alpha-amylase isozymes.
4622298 DETECTION AND QUANTITATION OF MICROORGANISMS, LEUKOCYTES AND SQUAMOUS EPITHELIAL CELLS IN URINE James D Mansour, Thomas H Schulte, Burton H Sage assigned to Becton Dickinson and Company A method for the assessment of bacteriuria and pyuria includes the simultaneous detection and quantitation of microorganisms, leukocytes and squamous epithelial cells in a urine specimen. The three cell types are stained with a fluorescent