4683206 Recombinant DNA cloning vectors containing selectable genetic markers for use in streptomyces and related organisms

4683206 Recombinant DNA cloning vectors containing selectable genetic markers for use in streptomyces and related organisms

314 PATENT ABSTRACTS pancreatic duct. Also provided in the invention is a method for production of monoclonal antibodies. The method comprises admin...

58KB Sizes 1 Downloads 118 Views

314

PATENT ABSTRACTS

pancreatic duct. Also provided in the invention is a method for production of monoclonal antibodies. The method comprises administering or implanting cancer cells or tissue to an animal having no thymus, propagating the cells or tissue in the animal, administering T cells or both T cells and B cells to the animal, taking antibodyproducing B cells from the animal, fusing the B cells with myeloma cells, selecting fused cells producing an antibody against the cancer cells or tissue, cloning the fused cells, and culturing the cloned fused cell.

4683205 METHOD FOR TRANSFORMING MICROORGANISMS Ryoichi Katsumata, Akio Ozaki, Tetsuo Oka, Akira Furuya, Machida, Japan assigned to Kyowa Hakko Kogyo Co Ltd Disclosed is a method for transformation of microorganisms belonging to the genera Corynebacterium and Brevibacterium whereby a foreign DNA may be introduced into a host cell and autonomously replicated.

4683206 RECOMBINANT DNA CLONING VECTORS CONTAINING SELECTABLE GENETIC MARKERS FOR USE IN STREPTOMYCES AND RELATED ORGANISMS James A Mabe, Walter M Nakatsukasa assigned to Eli Lilly and Company The present invention disclosed novel recombinant DNA cloning vectors for use in Streptomyces and related organisms. These novel cloning vectors contain genetic markers that provide antibiotic resistance or colorimetric selectivity to the host cells. The invention further comprises transformants of the aforementioned vectors.

4684611 PROCESS FOR THE IN-VITRO TRANSFORMATION OF PLANT PROTOPLASTS WITH PLASMID DNA Robbert A Schilperoort, Frans A Krens, George J Wultems, 2343 RP Oegstgeest, Netherlands assigned to Rijksuniversiteit Leiden: Schilperoort Robbert Genetic properties of higher plants are transformed by incubating protoplasts of the plants together with plasmid DNA. The incubation is effected in the presence of polyethylene glycol and calcium ions. Subsequently£13 during afterincubation + 13 the calcium ion concentration in the incubation medium is gradually increased and at the same time the polyethylene glycol concentration is decreased. The resulting aggregates are separated, grown further separately, and examined for modification of their genetic properties.

8701726 PROTOPLAST FUSION PRODUCT AND PROCESS FOR PREPARING SAME Tina Lorraine BARSBY, Roger John KEMBLE, 7 Armbro Avenue, Brampton, Ontario L6Y lW9, Canada assigned to A L L E L I X INC: Protoplast fusion techniques are employed to provide a progenitor plant which is cytoplasmically male sterile but which may also possess such traits as herbicide tolerance and a winter phenotype. Male sterility-conferring mitochondria of the rape plant are characterized genotypically. This allows identification of the parents of the desired fusion product and characterization of the fusion product itself. It also permits production of genotypically consistent seed by providing a means for accurately selecting the parents of a cross from which hybrid seed is produced.

8702038 VACCINES AND IMMUNOASSAYS FOR ACQUIRED IMMUNE DEFICIENCY SYNDROME Shiu-Lok HU, Anthony F PURCHIO, Linda MADISEN assigned to ONCOGEN