- Email: [email protected]
4950603 Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from streptomyces lipmanii
258
PATENT ABSTRACTS
leukemia cell line, preferably the AH-sensitive mutant of the Jurkat leukemia line identified as J3R7, with normal T cells and culturing the fusion product in a selective AH medium. Stable, interleukin-2 (IL-2)-producing human T-T hybridomas were made by this process.
4950599 METHOD FOR EXCHANGING HOMOLOGOUS DNA SEQUENCES IN A CELL USING POLYOMA ENCAPSULATED DNA FRAGMENTS Wol Bertling A method for altering a cell by exchanging a preselected cellular DNA sequence with an exogenous DNA sequence different from the cellular DNA sequence employs an exogenous DNA sequence encapsidated in a polyoma or polyoma-like capsid. The polyoma capsid is then contacted to the cell so that the exogenous DNA sequence is introduced within the cell and exchanges with the preselected cellular DNA sequence by homologous recombination. A preferred article of manufacture comprises a polyoma capsid and a plurality of DNA sequences encapsulated within the polyoma capsid. The D N A sequences each comprise not more than an incomplete portion of a single preselected gene. The exogenous DNA sequence may optionally be complexed to a DNA binding protein, such as a recA protein, prior to encapsulating the exogenous DNA sequence within a viral capsid, so that the uptake of the DNA sequence into the capsid is enhanced.
such as Streptomyces, Penicillium, and Cephalosporium. DNA compounds encoding the transcription and translation activating sequence and transcription termination sequence of the S. lipmanii IPNS gene are also useful in the construction of expression vectors, especially Streptomyces expression vectors. The S. lipmanii IPNS gene can be isolated from plasmid pOGO239, available from the Northern Regional Research Center under accession number NRRL B-18250.
4950604 CULTURE OF A MICROORGANSIM OF THE GENUS KLEBSIELLA SP., HAVING A HIGH CONTENT OF RHAMNOSE Marianne G P Graber-Gubert, Joseph C A Morin, Francis L Duchiron, Pierre F Monsan, Toulouse, France assigned to Bioeurope The invention relates to biotechnology. It relates, in particular, to cultures of Klebsiella sp. microorganisms possessing the characteristics of the strain BEC 441 deposited under No. 1-714 at the Collection Nationale de Cultures de Microorganismes (National Collection of Microorganism Cultures) of the Institut Pasteur, as well as strains obtained from the strain BEC 441 by mutation, these strains being capable of producing polysaccharides rich in rhamnose by fermentation in a nutrient medium containing assimilable sources of carbon and of nitrogen and inorganic substances. Application for the production of a mixture of monosaccharides having a high content of rhamnose.
4950603 4950646 RECOMBINANT DNA EXPRESSION VECTORS AND DNA COMPOUNDS THAT ENCODE ISOPENICILLIN N SYNTHETASE FROM STREPTOMYCES LIPMANII Thomas D Ingolia, Barbara J Weigel assigned to Eli Lilly and Company DNA compounds encoding the Streptomyces lipmanii isopenicillin N synthetase (IPNS) gene are useful for constructing a variety of recombinant DNA vectors. The vectors are useful in producing IPNS in a wide variety of host cells,
DNA SEQUENCES, RECOMBINANT DNA MOLECULES AND PROCESSES FOR PRODUCING HUMAN L1POCORTIN-LIKE POLYPEPTIDES Barbara Wallner, R Blak Pepinsky, Jeffrey Garwin, Daniel Schindler, Kuo-Sen Huang assigned to Biogen Inc DNA sequences, recombinant DNA molecules and hosts transformed with them which produce human lipocortin-like polypeptides and
PATENT ABSTRACTS
leukemia cell line, preferably the AH-sensitive mutant of the Jurkat leukemia line identified as J3R7, with normal T cells and culturing the fusion product in a selective AH medium. Stable, interleukin-2 (IL-2)-producing human T-T hybridomas were made by this process.
4950599 METHOD FOR EXCHANGING HOMOLOGOUS DNA SEQUENCES IN A CELL USING POLYOMA ENCAPSULATED DNA FRAGMENTS Wol Bertling A method for altering a cell by exchanging a preselected cellular DNA sequence with an exogenous DNA sequence different from the cellular DNA sequence employs an exogenous DNA sequence encapsidated in a polyoma or polyoma-like capsid. The polyoma capsid is then contacted to the cell so that the exogenous DNA sequence is introduced within the cell and exchanges with the preselected cellular DNA sequence by homologous recombination. A preferred article of manufacture comprises a polyoma capsid and a plurality of DNA sequences encapsulated within the polyoma capsid. The D N A sequences each comprise not more than an incomplete portion of a single preselected gene. The exogenous DNA sequence may optionally be complexed to a DNA binding protein, such as a recA protein, prior to encapsulating the exogenous DNA sequence within a viral capsid, so that the uptake of the DNA sequence into the capsid is enhanced.
such as Streptomyces, Penicillium, and Cephalosporium. DNA compounds encoding the transcription and translation activating sequence and transcription termination sequence of the S. lipmanii IPNS gene are also useful in the construction of expression vectors, especially Streptomyces expression vectors. The S. lipmanii IPNS gene can be isolated from plasmid pOGO239, available from the Northern Regional Research Center under accession number NRRL B-18250.
4950604 CULTURE OF A MICROORGANSIM OF THE GENUS KLEBSIELLA SP., HAVING A HIGH CONTENT OF RHAMNOSE Marianne G P Graber-Gubert, Joseph C A Morin, Francis L Duchiron, Pierre F Monsan, Toulouse, France assigned to Bioeurope The invention relates to biotechnology. It relates, in particular, to cultures of Klebsiella sp. microorganisms possessing the characteristics of the strain BEC 441 deposited under No. 1-714 at the Collection Nationale de Cultures de Microorganismes (National Collection of Microorganism Cultures) of the Institut Pasteur, as well as strains obtained from the strain BEC 441 by mutation, these strains being capable of producing polysaccharides rich in rhamnose by fermentation in a nutrient medium containing assimilable sources of carbon and of nitrogen and inorganic substances. Application for the production of a mixture of monosaccharides having a high content of rhamnose.
4950603 4950646 RECOMBINANT DNA EXPRESSION VECTORS AND DNA COMPOUNDS THAT ENCODE ISOPENICILLIN N SYNTHETASE FROM STREPTOMYCES LIPMANII Thomas D Ingolia, Barbara J Weigel assigned to Eli Lilly and Company DNA compounds encoding the Streptomyces lipmanii isopenicillin N synthetase (IPNS) gene are useful for constructing a variety of recombinant DNA vectors. The vectors are useful in producing IPNS in a wide variety of host cells,
DNA SEQUENCES, RECOMBINANT DNA MOLECULES AND PROCESSES FOR PRODUCING HUMAN L1POCORTIN-LIKE POLYPEPTIDES Barbara Wallner, R Blak Pepinsky, Jeffrey Garwin, Daniel Schindler, Kuo-Sen Huang assigned to Biogen Inc DNA sequences, recombinant DNA molecules and hosts transformed with them which produce human lipocortin-like polypeptides and