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4707357 Immunologically active peptides capable of inducing immunization against malaria and genes encoding therefor
226
PATENT ABSTRACTS 4705752
PROCESS FOR THE ENZYMATIC HYDROLYSIS OF D- ALPHAAMINO-ACID AMIDES Wilhelmus H J Boesten, Maria J Cals, Sittard, Netherlands assigned to Stamicarbon B V Process for the enzymatic hydrolysis of a Dalpha-amino-acid amide to the corresponding D- alpha-amino-acid, wherein an aqueous solution of the D- alpha-amino-acid amide is contacted with an aminoacylamidase-containing preparation obtained from a culture of Rhodococcus erythropolis or a mutant thereof and the D- alpha-amino-acid is subsequently recovered from the hydrolysate obtained.
4705848 ISOLATION OF BIOACTIVE, MONOMERIC GROWTH HORMONE Ren-De Yang, Edwin J Hamilton, Larry D Taber assigned to International Minerals & Chemical Corp Monomeric, biologically active growth hormone is isolated from microbially-produced insoluble inclusion bodies by solubilizing and denaturing the growth hormone by extraction of the inclusion bodies into a guanidine salt solution such as guanidine hydrochloride and subsequently renaturing at least a portion of the growth hormone in the solution by replacing the guanidine salt solution with a denaturant-free buffer solution and removing precipitated impurities and growth hormone aggregates. The renatured growth hromone is then purified by ion-exchange chromatography.
4706192 INPUT APPARATUS FOR ENTERING BASE SEQUENCE INFORMATION OF THE GENE Hisanori Nasu, Satoru Suzuki, Hiroak Yoshida, Tomoich Fukuda, Eiichi Soeda, Yokohama, Japan assigned to Hitachi Software Engineering Co Ltd An input apparatus for entering base sequence information of the gene, comprises a computer
for analyzing the base sequence of the gene, an input apparatus for entering base sequence information of the gene to the computer which successively stores the base sequence information entered by the input apparatus and produces the stored base sequence information, and a speech sounding apparatus supplied with the base sequence information produced from the computer for sounding speech corresponding to the base sequence information produced from the computer. 4707167 AIR STERILIZATION
FILTER
Kenichiro Saito, Chikao Kanaoka, Shigeyuki Aoyama, Tokyo, Japan assigned to Aoki Corporation An air sterilization filter for the containment of leakage prevention of biologically contaminated air in space for a biotechnological performance, such as a genetic engineering work. The filter comprises a heat durable sheet filter for collecting microorganisms suspended in air and a heater for heating the sheet filter and sterilizing the collected microorganisms. The filter of the invention is also useful for providing a clean room. 4707357 IMMUNOLOGICALLY ACTIVE PEPTIDES CAPABLE OF INDUCING IMMUNIZATION AGAINST MALARIA AND GENES ENCODING THEREFOR John B Dame, Jackie Williams, Thomas F McCutchan, Imogen Schneider assigned to The United States of America as represented by the Secretary of the Army An immunologically active substantially pure peptide capable of inducing in a human an immune response which is cross reactive with and protective against infection by a malaria parasite, wherein the peptide contains at least 2 consecutive repeats of a sequence Asn-X-Y-Pro wherein X is Ala or Val and Y is Ash or Asp or a sequence of the formula Thr-Glu-Trp-Z-ProCys-Ser-Val-Thr-Cys-Gly-Asn-Glywherein Z is Ser or Thr or the formula Lys-Pro-S-T-S-LysLeu-Lys-Gln-Pro-U-V-Gly-W-Pro wherein S is Lys or Asn, T is His or Glu, U is Gly or Asn, V is As or Glu, and W is Asn or Gin is disclosed along
227
PATENT ABSTRACTS with D N A sequences and various other genetic materials useful in producing these peptides through biological methods. 4707358 VACCINE
AGAINST EPSTEINBARR VIRUS
Elliott Kieff, Jerome Tanner, Mary Hummel, Christopher Beisel assigned to The University of Chicago The nucleotide sequence of Epstein-Barr Virus (EBV) DNA which codes for outer surface viral proteins has been determined. Fragments of the DNA have been isolated and cloned into a vector which, when placed in a host organism, express proteins which when used to immunize rabbits generate antibody which reacts with the surface proteins of virus infected cells. These proteins are useful for preparation of a vaccine for EBV.
47~4~ IMMUNOASSAY FOR BREAST CANCER EMPLOYING MONOCLONAL ANTIBODIES Iafa Keydar, Tel Aviv, Israel assigned to Tel Aviv University; Teva Pharmaceutical Industries Limite An immunoassay for diagnosing and monitoring human breast cancer. The assay employs a monoclonal antibody which recognizes a human mammary tumor virus derived from the T47D clone-10 breast cancer cell line (HMTV). The monoclonal antibody is produced by the hybridoma cell line deposited with the American Type Culture Collection under ATCC Accession No. HB 8630. When the immunoassay is performed on a tissue sample from a subject, the sample is contacted with monoclonal antibody ATCC Accession No. HB 8630 so as to form an antibody-antigen complex between the monoclonal antibody and any HMTV antigens which may be present in the sample. This antibodyantigen complex is then detected employing a second detectable antibody specific to the first monoclonal antibody. The presence or absence of the complex indicates the presence or absence of breast cancer in the subject. When the immunoassay is performed on a body fluid sample from a subject, the sample is first contacted with a detectable soluble antibody so as to form an antibody-antigen complex between the anti-
body and any HMTV antigens which may be present in the sample. The fluid sample is then contacted by a second antibody which is attached to a solid support. Either the first or second antibody is the monoclonal antibody produced by hybridoma cell line ATCC Accession No. HB 8630. The presence or absence of the complex indicates the presence or absence of human breast cancer in the subject.
~07~5 INTACT GENE AND METHOD OF EXCISING AND CLONING SAME Thomas F McCutchan, John B Dame assigned to The United States of America as represented by the Secretary of the Department of Health and Human Services The present invention discloses isolated functionally intact whole genes and method of obtaining the same. The method includes treating genomic DNA with mung bean nuclease and formamide under controlled conditions. The invention also discloses cloning of said intact whole genes and a library of such cloned genes or any recombinations thereof. The invention is useful in deriving gene products as m-RNA, S-RNA, tRNA, polypeptides and the like.
4707448 IMMORTAL LINE OF HUMAN FETAL GLIAL CELLS Eugene Major assigned to The United States of America as represented by the Department of Health and Human Services The present invention discloses permanent establishment of an immortal line of fetal glial cells. The fetal glial cell line is capable of mitotically proliferating and continually growing in vitro under suitable culture media and environmental conditions. A method of producing immortalized human fetal glial cell line is also disclosed. The method comprises transfecting primary human fetal glial cells with an origin-defective mutant of SV40 virus, passaging the resulting astroglial cells through suitable number of cycles and obtaining the desired cell line. The cell line of the present invention is capable of supporting multiplication of JC virus.
PATENT ABSTRACTS 4705752
PROCESS FOR THE ENZYMATIC HYDROLYSIS OF D- ALPHAAMINO-ACID AMIDES Wilhelmus H J Boesten, Maria J Cals, Sittard, Netherlands assigned to Stamicarbon B V Process for the enzymatic hydrolysis of a Dalpha-amino-acid amide to the corresponding D- alpha-amino-acid, wherein an aqueous solution of the D- alpha-amino-acid amide is contacted with an aminoacylamidase-containing preparation obtained from a culture of Rhodococcus erythropolis or a mutant thereof and the D- alpha-amino-acid is subsequently recovered from the hydrolysate obtained.
4705848 ISOLATION OF BIOACTIVE, MONOMERIC GROWTH HORMONE Ren-De Yang, Edwin J Hamilton, Larry D Taber assigned to International Minerals & Chemical Corp Monomeric, biologically active growth hormone is isolated from microbially-produced insoluble inclusion bodies by solubilizing and denaturing the growth hormone by extraction of the inclusion bodies into a guanidine salt solution such as guanidine hydrochloride and subsequently renaturing at least a portion of the growth hormone in the solution by replacing the guanidine salt solution with a denaturant-free buffer solution and removing precipitated impurities and growth hormone aggregates. The renatured growth hromone is then purified by ion-exchange chromatography.
4706192 INPUT APPARATUS FOR ENTERING BASE SEQUENCE INFORMATION OF THE GENE Hisanori Nasu, Satoru Suzuki, Hiroak Yoshida, Tomoich Fukuda, Eiichi Soeda, Yokohama, Japan assigned to Hitachi Software Engineering Co Ltd An input apparatus for entering base sequence information of the gene, comprises a computer
for analyzing the base sequence of the gene, an input apparatus for entering base sequence information of the gene to the computer which successively stores the base sequence information entered by the input apparatus and produces the stored base sequence information, and a speech sounding apparatus supplied with the base sequence information produced from the computer for sounding speech corresponding to the base sequence information produced from the computer. 4707167 AIR STERILIZATION
FILTER
Kenichiro Saito, Chikao Kanaoka, Shigeyuki Aoyama, Tokyo, Japan assigned to Aoki Corporation An air sterilization filter for the containment of leakage prevention of biologically contaminated air in space for a biotechnological performance, such as a genetic engineering work. The filter comprises a heat durable sheet filter for collecting microorganisms suspended in air and a heater for heating the sheet filter and sterilizing the collected microorganisms. The filter of the invention is also useful for providing a clean room. 4707357 IMMUNOLOGICALLY ACTIVE PEPTIDES CAPABLE OF INDUCING IMMUNIZATION AGAINST MALARIA AND GENES ENCODING THEREFOR John B Dame, Jackie Williams, Thomas F McCutchan, Imogen Schneider assigned to The United States of America as represented by the Secretary of the Army An immunologically active substantially pure peptide capable of inducing in a human an immune response which is cross reactive with and protective against infection by a malaria parasite, wherein the peptide contains at least 2 consecutive repeats of a sequence Asn-X-Y-Pro wherein X is Ala or Val and Y is Ash or Asp or a sequence of the formula Thr-Glu-Trp-Z-ProCys-Ser-Val-Thr-Cys-Gly-Asn-Glywherein Z is Ser or Thr or the formula Lys-Pro-S-T-S-LysLeu-Lys-Gln-Pro-U-V-Gly-W-Pro wherein S is Lys or Asn, T is His or Glu, U is Gly or Asn, V is As or Glu, and W is Asn or Gin is disclosed along
227
PATENT ABSTRACTS with D N A sequences and various other genetic materials useful in producing these peptides through biological methods. 4707358 VACCINE
AGAINST EPSTEINBARR VIRUS
Elliott Kieff, Jerome Tanner, Mary Hummel, Christopher Beisel assigned to The University of Chicago The nucleotide sequence of Epstein-Barr Virus (EBV) DNA which codes for outer surface viral proteins has been determined. Fragments of the DNA have been isolated and cloned into a vector which, when placed in a host organism, express proteins which when used to immunize rabbits generate antibody which reacts with the surface proteins of virus infected cells. These proteins are useful for preparation of a vaccine for EBV.
47~4~ IMMUNOASSAY FOR BREAST CANCER EMPLOYING MONOCLONAL ANTIBODIES Iafa Keydar, Tel Aviv, Israel assigned to Tel Aviv University; Teva Pharmaceutical Industries Limite An immunoassay for diagnosing and monitoring human breast cancer. The assay employs a monoclonal antibody which recognizes a human mammary tumor virus derived from the T47D clone-10 breast cancer cell line (HMTV). The monoclonal antibody is produced by the hybridoma cell line deposited with the American Type Culture Collection under ATCC Accession No. HB 8630. When the immunoassay is performed on a tissue sample from a subject, the sample is contacted with monoclonal antibody ATCC Accession No. HB 8630 so as to form an antibody-antigen complex between the monoclonal antibody and any HMTV antigens which may be present in the sample. This antibodyantigen complex is then detected employing a second detectable antibody specific to the first monoclonal antibody. The presence or absence of the complex indicates the presence or absence of breast cancer in the subject. When the immunoassay is performed on a body fluid sample from a subject, the sample is first contacted with a detectable soluble antibody so as to form an antibody-antigen complex between the anti-
body and any HMTV antigens which may be present in the sample. The fluid sample is then contacted by a second antibody which is attached to a solid support. Either the first or second antibody is the monoclonal antibody produced by hybridoma cell line ATCC Accession No. HB 8630. The presence or absence of the complex indicates the presence or absence of human breast cancer in the subject.
~07~5 INTACT GENE AND METHOD OF EXCISING AND CLONING SAME Thomas F McCutchan, John B Dame assigned to The United States of America as represented by the Secretary of the Department of Health and Human Services The present invention discloses isolated functionally intact whole genes and method of obtaining the same. The method includes treating genomic DNA with mung bean nuclease and formamide under controlled conditions. The invention also discloses cloning of said intact whole genes and a library of such cloned genes or any recombinations thereof. The invention is useful in deriving gene products as m-RNA, S-RNA, tRNA, polypeptides and the like.
4707448 IMMORTAL LINE OF HUMAN FETAL GLIAL CELLS Eugene Major assigned to The United States of America as represented by the Department of Health and Human Services The present invention discloses permanent establishment of an immortal line of fetal glial cells. The fetal glial cell line is capable of mitotically proliferating and continually growing in vitro under suitable culture media and environmental conditions. A method of producing immortalized human fetal glial cell line is also disclosed. The method comprises transfecting primary human fetal glial cells with an origin-defective mutant of SV40 virus, passaging the resulting astroglial cells through suitable number of cycles and obtaining the desired cell line. The cell line of the present invention is capable of supporting multiplication of JC virus.