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4731327 Method for stabilizing recombinant DNA molecules and enhancing the expression thereof in strains of bacillus
PATENT ABSTRACTS
4729951 PROCESS FOR THE IMPROVEMENT OF ANTIBIOTIC PRODUCTION BY IN VIVO GENETIC RECOMBINATION Lajos Ferenczy, Antal Mai, Istvan Ott, Gabor Ambrus, Tibor Lang, Szeged, Hungary assigned to Biogal Gyogyszergyar A process for the production of aminoglycoside antibiotics using fused protoplasts derived from streptomyces. The cells are precultured in a medium containing sucrose, calcium and magnesium salts. Protoplasts are formed and then fused. The fused protoplasts are regenerate and antibiotic producing ability is screened for. The regenerate cells produced an antibiotic complex of modified composition or demonstrated increased or reduced antibiotic productivity compared to the fusion partners.
4729954 PLS010 PLASMID
VECTOR
Sanford A Lacks, Tanjore S Balganesh assigned to The United States of America as represented by the United States Department of Energy Disclosed is recombinant plasmid pLS 101, consisting essentially of a 2.0 Kb maiM gene fragment ligated to a 4.4 Kb Tcr D N A fragment, which is particularly useful for transforming Gram-positive bacteria. This plasmid contains at least four restriction sites suitable for inserting exogeneous gene sequences. Also disclosed is a method for plasmid isolation by penicillin selection, as well as processes for enrichment of recombinant plasmids in Gram-positive bacterial systems.
241
DNA techniques and their use in sandwich hybridization methods is also described. By making different combinations of the nucleic acid fragments-some labeled and some affixed to solid carriers, it is possible to create kits for the identification of e.g. veneral diseases. The improved nucleic acid reagents comprise two series of at least two alternating nucleic acid fragments, which are homologous to sequences in the nucleic acid to be identified, one of the series being labeled and one affixed to a solid carrier. Nucleic acid fragments belonging to different series must not be homologous to each other. Sandwich hybridization tests performed with arrays of nucleic acid fragments are at least four times as sensitive as sandwich hybridization tests performed with reagents belonging to the prior art. 4731327 METHOD FOR STABILIZING RECOMBINANT DNA MOLECULES AND ENHANCING THE EXPRESSION THEREOF IN STRAINS OF BACILLUS Yoshi Furutani, Masaru Honjo, Kazuaki Manabe, Hiroak Shimada, Noboru Tomioka, Miura, Japan assigned to Agency of Industrial Science and Technology; Ministry of International Trade and Indust A recombinant DNA molecule, a portion of which includes a vector whose replication is initiated independently of the temperaturesensitive mutant gene of a temperature-sensitive mutant strain of the genus Bacillus as host is introduced into the host. The host is then cultured at a temperature which does not completely inhibit chromosomal D N A replication. By this process, the recombinant DNA molecule can be stabilized in the host and its expression can be enhanced.
4731325 4731499 ARRAYS OF ALTERNATING NUCLEIC ACID FRAGMENTS FOR HYBRIDIZATION ARRAYS
HYBRID CORN PLANT AND SEED
Airi Palva, Tuula M Ranki, Hans Soderlund, Helsinki, Finland assigned to Orion-yhtyma
Vladimir Puskaric, Lori Carrigan, Woodstock, MN, Canada assigned to Pioneer Hi-Bred International Inc
The invention is related to improved nucleic acid reagents comprising arrays of nucleic acid fragments and combinations of such fragments. The preparation of such fragments by recombinant
According to the invention, there is provided a hybrid corn plant, designated 3790, produced by crossing two Pioneer Hi-Bred International, Inc. proprietary inbred lines of corn. This invention
4729951 PROCESS FOR THE IMPROVEMENT OF ANTIBIOTIC PRODUCTION BY IN VIVO GENETIC RECOMBINATION Lajos Ferenczy, Antal Mai, Istvan Ott, Gabor Ambrus, Tibor Lang, Szeged, Hungary assigned to Biogal Gyogyszergyar A process for the production of aminoglycoside antibiotics using fused protoplasts derived from streptomyces. The cells are precultured in a medium containing sucrose, calcium and magnesium salts. Protoplasts are formed and then fused. The fused protoplasts are regenerate and antibiotic producing ability is screened for. The regenerate cells produced an antibiotic complex of modified composition or demonstrated increased or reduced antibiotic productivity compared to the fusion partners.
4729954 PLS010 PLASMID
VECTOR
Sanford A Lacks, Tanjore S Balganesh assigned to The United States of America as represented by the United States Department of Energy Disclosed is recombinant plasmid pLS 101, consisting essentially of a 2.0 Kb maiM gene fragment ligated to a 4.4 Kb Tcr D N A fragment, which is particularly useful for transforming Gram-positive bacteria. This plasmid contains at least four restriction sites suitable for inserting exogeneous gene sequences. Also disclosed is a method for plasmid isolation by penicillin selection, as well as processes for enrichment of recombinant plasmids in Gram-positive bacterial systems.
241
DNA techniques and their use in sandwich hybridization methods is also described. By making different combinations of the nucleic acid fragments-some labeled and some affixed to solid carriers, it is possible to create kits for the identification of e.g. veneral diseases. The improved nucleic acid reagents comprise two series of at least two alternating nucleic acid fragments, which are homologous to sequences in the nucleic acid to be identified, one of the series being labeled and one affixed to a solid carrier. Nucleic acid fragments belonging to different series must not be homologous to each other. Sandwich hybridization tests performed with arrays of nucleic acid fragments are at least four times as sensitive as sandwich hybridization tests performed with reagents belonging to the prior art. 4731327 METHOD FOR STABILIZING RECOMBINANT DNA MOLECULES AND ENHANCING THE EXPRESSION THEREOF IN STRAINS OF BACILLUS Yoshi Furutani, Masaru Honjo, Kazuaki Manabe, Hiroak Shimada, Noboru Tomioka, Miura, Japan assigned to Agency of Industrial Science and Technology; Ministry of International Trade and Indust A recombinant DNA molecule, a portion of which includes a vector whose replication is initiated independently of the temperaturesensitive mutant gene of a temperature-sensitive mutant strain of the genus Bacillus as host is introduced into the host. The host is then cultured at a temperature which does not completely inhibit chromosomal D N A replication. By this process, the recombinant DNA molecule can be stabilized in the host and its expression can be enhanced.
4731325 4731499 ARRAYS OF ALTERNATING NUCLEIC ACID FRAGMENTS FOR HYBRIDIZATION ARRAYS
HYBRID CORN PLANT AND SEED
Airi Palva, Tuula M Ranki, Hans Soderlund, Helsinki, Finland assigned to Orion-yhtyma
Vladimir Puskaric, Lori Carrigan, Woodstock, MN, Canada assigned to Pioneer Hi-Bred International Inc
The invention is related to improved nucleic acid reagents comprising arrays of nucleic acid fragments and combinations of such fragments. The preparation of such fragments by recombinant
According to the invention, there is provided a hybrid corn plant, designated 3790, produced by crossing two Pioneer Hi-Bred International, Inc. proprietary inbred lines of corn. This invention