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5045471 Cloned DNA for P450SCC and expression thereof
New Patents
sertion of nptI1 promoter sequences in nodK upstream of nodABC so as to activate nodABC. As compared with inoculation with the corresponding wild-type Bradyrhizobium sp. (Parasponia), nodulation onset occurred live days earlier, nodulation number was doubled, and a 120% enhancement of plant yield was shown. Similar results were obtained regardless of the orientation of the nptI1 promoter-containing sequences within the nodK coding sequence.
active B. thuringiensis microbe. The DNA encoding the B.t. toxin can be used to transform various prokaryotic and eukaryotic microbes to express the B.t. toxin. These r~omb~nant microbes can be used to control lepidopteran insects in various environments.
5045471 CLONED DNA FOR P45OSCC AND EXPRESSION THEREOF
5045463 DNA EXPRESSION VECTOR AND USE THEREOF Michael A Innis, David H Gelfand, Meade assigned to Cetus Corporation
111
James
A gene having a DNA sequence complementary to that of the glucoamylase polypeptide mRNA from a fungal species, preferably Aspergillus awamori, is prepared. The mRNA is an approximately 2.2 kilobase poly A RNA obtained from of fungal cells grown under conditions glucoamylase induction. Reverse transcription of the mRNA provides a glucoamylase probe used to identify genomic digest fragments containing glucoamylase gene regions, which are sequenced to locate the introns and exons. The genomic fragments are spliced together to form a gene having a DNA sequence with altered or deleted introns which codes for fungal glucoamylase protein and is capable, when correctly combined with a cleaved DNA expression vector, of expressing a non-native protein having glucoamylase enzyme activity upon transformation of a host organism by the vector. The host is preferably bacteria or yeast. The transformed yeast host may be used to produce ethanol.
5045469 NOVEL BACILLUS THURINGIENSIS ISOLATE DENOTED B. T. PStilF, ACTIVE AGAINST LEPIDOPTERAN PESTS, AND A GENE ENCODING A LEPIDOPTERAN-ACTIVE TOXIN Jewel Payne, August J Sick assigned to Mycogen Corporation A novel B.t. toxin gene toxic to lepidopteran insects has been cloned from a novel lepidopteran-
Walter Miller assigned to The Regents of the University of California A polynucleotide sequence encoding human P45Oscc is disclosed along with techniques for utilizing the P45Oscc expressed by this gene to reduce in vivo cholesterol levles.
5045479 CONTINUOUS FLOW COMPETITIVE ASSAY WITH REFERENCE SYSTEM Arnold L Newman, William Stanbro assigned to The Johns Hopkins University The invention relates to a continuous flow competitive assay system for the detection and measurement of chemical and biochemical analytes. It is a time-based, continuous on-line measurement of analyte concentrations comprising three functional assemblies connected in series: a sampler, a reactor and a detector. Tagged immunochemical discharged from the reactor is detected in the detector which contains a model of system response with the tagged immunochemical and keeps track of the amount of tagged immunochemical lost during the course of the operation of the reactor.
5045633 EXPRESSION OF BIOLOGICALLY ACTIVE PDGF ANALOGS IN EUCARYOTIC CELLS Mark Murray, James ZymoGenetics Inc
Kelly
assigned
to
Biologically active PDGF analogs expressed in eucaryotic cells are disclosed. The analogs are produced by yeast strains transform with an
sertion of nptI1 promoter sequences in nodK upstream of nodABC so as to activate nodABC. As compared with inoculation with the corresponding wild-type Bradyrhizobium sp. (Parasponia), nodulation onset occurred live days earlier, nodulation number was doubled, and a 120% enhancement of plant yield was shown. Similar results were obtained regardless of the orientation of the nptI1 promoter-containing sequences within the nodK coding sequence.
active B. thuringiensis microbe. The DNA encoding the B.t. toxin can be used to transform various prokaryotic and eukaryotic microbes to express the B.t. toxin. These r~omb~nant microbes can be used to control lepidopteran insects in various environments.
5045471 CLONED DNA FOR P45OSCC AND EXPRESSION THEREOF
5045463 DNA EXPRESSION VECTOR AND USE THEREOF Michael A Innis, David H Gelfand, Meade assigned to Cetus Corporation
111
James
A gene having a DNA sequence complementary to that of the glucoamylase polypeptide mRNA from a fungal species, preferably Aspergillus awamori, is prepared. The mRNA is an approximately 2.2 kilobase poly A RNA obtained from of fungal cells grown under conditions glucoamylase induction. Reverse transcription of the mRNA provides a glucoamylase probe used to identify genomic digest fragments containing glucoamylase gene regions, which are sequenced to locate the introns and exons. The genomic fragments are spliced together to form a gene having a DNA sequence with altered or deleted introns which codes for fungal glucoamylase protein and is capable, when correctly combined with a cleaved DNA expression vector, of expressing a non-native protein having glucoamylase enzyme activity upon transformation of a host organism by the vector. The host is preferably bacteria or yeast. The transformed yeast host may be used to produce ethanol.
5045469 NOVEL BACILLUS THURINGIENSIS ISOLATE DENOTED B. T. PStilF, ACTIVE AGAINST LEPIDOPTERAN PESTS, AND A GENE ENCODING A LEPIDOPTERAN-ACTIVE TOXIN Jewel Payne, August J Sick assigned to Mycogen Corporation A novel B.t. toxin gene toxic to lepidopteran insects has been cloned from a novel lepidopteran-
Walter Miller assigned to The Regents of the University of California A polynucleotide sequence encoding human P45Oscc is disclosed along with techniques for utilizing the P45Oscc expressed by this gene to reduce in vivo cholesterol levles.
5045479 CONTINUOUS FLOW COMPETITIVE ASSAY WITH REFERENCE SYSTEM Arnold L Newman, William Stanbro assigned to The Johns Hopkins University The invention relates to a continuous flow competitive assay system for the detection and measurement of chemical and biochemical analytes. It is a time-based, continuous on-line measurement of analyte concentrations comprising three functional assemblies connected in series: a sampler, a reactor and a detector. Tagged immunochemical discharged from the reactor is detected in the detector which contains a model of system response with the tagged immunochemical and keeps track of the amount of tagged immunochemical lost during the course of the operation of the reactor.
5045633 EXPRESSION OF BIOLOGICALLY ACTIVE PDGF ANALOGS IN EUCARYOTIC CELLS Mark Murray, James ZymoGenetics Inc
Kelly
assigned
to
Biologically active PDGF analogs expressed in eucaryotic cells are disclosed. The analogs are produced by yeast strains transform with an