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4772557 DNA clone of human skin fibroblast collagenase enzyme
70
PATENT ABSTRACTS
correlated are two insertion polymorphisms 5" of the insulin gene.
DNA CLONE OF HUMAN SKIN FIBROBLAST COLLAGENASE ENZYME
4772555 DEDICATED RIBOSOMES THEIR USE
4772557
AND
Herman A De Boer assigned to Genentech Inc A method is described that dedicates specialized ribosomes to the synthesis of desired proteins. DNA which encodes rRNA having a mutant anti-Shine-Dalgarno sequence is used to transform a host cell in combination with DNA encoding messsenger RNA for the desired protein having a complementary mutant ShineDalgarno sequence. Since the mutant sequences are selected so as to be substantially unrecognized by either endogenous messenger RNA or ribosomes, respectively, the synthesis of protein proceeds in a dedicated system without interference from other host cell protein synthetic machinery. 4772556 GENETICALLY STABLE ALLOPOLYPLOID SOMATIC FUSION PRODUCT USEFUL IN THE PRODUCTION OF FUEL ALCOHOLS Graham G Stewart, lngeborg Russell, Chandrakant J Panchal, London, Canada assigned to Labatt Brewing Company Limited The present invention relates to a stable, allopolyploid, somatic fusion product useful in the fermentation arts and especially in the production of fuel alcohols. More particularly, the present invention relates to a novel allopolyploid yeast strain, Saccharomyces diastaticus NCYC 1460, which is the product of the spheroplast fusion of: (a) a hybrid, diploid strain of Sacch. diastaticus; and (b) a fusion partner which is a polyploid brewing yeast strain of the species Saccharomyces uvarum (carlsbergensis). The fusion product of the present invention is capable of an enhanced rate of fermentation and a generally higher degree of final attenuation than is typical of either of the fusion partners. Moreover the fusion product also evidences enhanced osmo- and thermo-tolerances and its multiple, non-allelic DEX gene complement renders the organism well suited to the fermentation of substrates containing dextrins and oligosaccharides.
Arthur Z Eisen, Gregory 1 Goldberg, Eugene Bauer assigned to Washington University A cDNA having a base sequence for human skin fibroblast collagenase has been cloned and characterized and the amino acid sequence of the human skin fibroblast protein has been determined.
4772691 CHEMICALLY CLEAVABLE NUCLEOTIDES Timothy M Herman assigned to The Medical College of Wisconsin Inc Novel biotinylated nucleotides have a chemically cleavable linker arm between a biotin and an organic basic group. They are useful in a method of isolating target macromolecules from crude physiological mixtures. The biotinylated nucleotides are bound via their organic basic groups to macromolecules having an affinity for the target macromolecules and brought into contact with the target macromolecules to form a biotinylated nucleotide-affinity macromolecule target macromolecule complex. The complex thus obtained is brought into contact with immobilized avidin whereupon the biotin moeity binds to the avidin. The complex and avidin are washed to remove undesired substances and then the chemically cleavable bond in the nucleotide is cleaved to obtain the affinity-macromolecule target macromolecule complex from which the target macromolecule can be obtained.
4774175 IMMUNOCHEMICAL METHODS FOR THE DETECTION OF ANTIBODY AGAINST HTLV-III Tse W Chang, Ikunoshi Kato, Pranab Chanda, Nancy Chang assigned to Centocor Inc Gene segments of human T cell lymphotropic virus type III (HTLV-III) were expressed in E. coli as peptides that are reactive with sera from patients with acquired immune deficiency syn-
PATENT ABSTRACTS
correlated are two insertion polymorphisms 5" of the insulin gene.
DNA CLONE OF HUMAN SKIN FIBROBLAST COLLAGENASE ENZYME
4772555 DEDICATED RIBOSOMES THEIR USE
4772557
AND
Herman A De Boer assigned to Genentech Inc A method is described that dedicates specialized ribosomes to the synthesis of desired proteins. DNA which encodes rRNA having a mutant anti-Shine-Dalgarno sequence is used to transform a host cell in combination with DNA encoding messsenger RNA for the desired protein having a complementary mutant ShineDalgarno sequence. Since the mutant sequences are selected so as to be substantially unrecognized by either endogenous messenger RNA or ribosomes, respectively, the synthesis of protein proceeds in a dedicated system without interference from other host cell protein synthetic machinery. 4772556 GENETICALLY STABLE ALLOPOLYPLOID SOMATIC FUSION PRODUCT USEFUL IN THE PRODUCTION OF FUEL ALCOHOLS Graham G Stewart, lngeborg Russell, Chandrakant J Panchal, London, Canada assigned to Labatt Brewing Company Limited The present invention relates to a stable, allopolyploid, somatic fusion product useful in the fermentation arts and especially in the production of fuel alcohols. More particularly, the present invention relates to a novel allopolyploid yeast strain, Saccharomyces diastaticus NCYC 1460, which is the product of the spheroplast fusion of: (a) a hybrid, diploid strain of Sacch. diastaticus; and (b) a fusion partner which is a polyploid brewing yeast strain of the species Saccharomyces uvarum (carlsbergensis). The fusion product of the present invention is capable of an enhanced rate of fermentation and a generally higher degree of final attenuation than is typical of either of the fusion partners. Moreover the fusion product also evidences enhanced osmo- and thermo-tolerances and its multiple, non-allelic DEX gene complement renders the organism well suited to the fermentation of substrates containing dextrins and oligosaccharides.
Arthur Z Eisen, Gregory 1 Goldberg, Eugene Bauer assigned to Washington University A cDNA having a base sequence for human skin fibroblast collagenase has been cloned and characterized and the amino acid sequence of the human skin fibroblast protein has been determined.
4772691 CHEMICALLY CLEAVABLE NUCLEOTIDES Timothy M Herman assigned to The Medical College of Wisconsin Inc Novel biotinylated nucleotides have a chemically cleavable linker arm between a biotin and an organic basic group. They are useful in a method of isolating target macromolecules from crude physiological mixtures. The biotinylated nucleotides are bound via their organic basic groups to macromolecules having an affinity for the target macromolecules and brought into contact with the target macromolecules to form a biotinylated nucleotide-affinity macromolecule target macromolecule complex. The complex thus obtained is brought into contact with immobilized avidin whereupon the biotin moeity binds to the avidin. The complex and avidin are washed to remove undesired substances and then the chemically cleavable bond in the nucleotide is cleaved to obtain the affinity-macromolecule target macromolecule complex from which the target macromolecule can be obtained.
4774175 IMMUNOCHEMICAL METHODS FOR THE DETECTION OF ANTIBODY AGAINST HTLV-III Tse W Chang, Ikunoshi Kato, Pranab Chanda, Nancy Chang assigned to Centocor Inc Gene segments of human T cell lymphotropic virus type III (HTLV-III) were expressed in E. coli as peptides that are reactive with sera from patients with acquired immune deficiency syn-