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New Patents donor tissue of field grown Zea diploperennis, a diploid, perennial corn ancestor with high tillering capacity. This species is used as a parent in a maize improvement strategy to transfer the unique traits of high tillering and plantlet regeneration capacity into cultivated con. After 3-4 subcultures of cultured somatic tissues on a primary medium, small callus fragments are transferred to a secondary medium devoid of the auxin, 2,4-D. After a few days, numerous shoots regenerate and develop into normal plantlets which are then separated and transferred to a tertiary medium for root development. The selection of somaclonal variants form cultured somatic cells of interspecific hybrids between corn and teosinte are used for the synthesis of unique breeding lines suited for development of improved corn varieties. A protocol for gene transfer employing recombinant DNA techniques is also described.
4811109 IMAGE PROCESSING SYSTEM Shibata, Takehiko Shimizu, Katsuichi Yoshikazu Yokomizo, Akira Suzuki, Koichi Tadashi Yoshida, Masaharu Murakami, Tsukada, Nao Nagashima, Ken Miyagi, Kunitachi, Japan assigned to Canon Kabushiki Kaisha An image processing system of the invention has a reader which reads out an image of an original, a page memory which stores image information of the original in the form of electric signals, a disk memory which stores part or all of the image information in the disk memory and also stores image processing information, an image processing section, a digitizer for the operator to input the image processing information, a CRT which displays input information or corrections in conversation language, a DMA controller, and a printer. The image processing system of the invention is capable of DNA transfer without the intermediacy of a CPU. Fewer addresses are required for readout of the image information from the memory, and high speed image processing is achieved.
4811218 REAL TIME SCANNING ELECTROPHORESIS APPARATUS FOR DNA SEQUENCING Michael Hunkapiller, Charles Connell, William Mordan, John Lytle, John Bridgham assigned to Applied Biosystems Inc
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A real-time, automated, nucleic acid sequencing apparatus that offers high speed, definitive sequencing on many samples at the same time. The apparatus permits more than one clone to be sequenced at a time, thus vastly decreasing the time required to sequence longer fragments and reducing sequencing costs accordingly. The apparatus detects electromagnetic radiation from a plurality of lanes in an electrophoresis system wherein the plurality of lanes are arranged in a planar array. The apparatus includes an optical system for detecting the radiation at a plurality of wavelengths and is made up of a collection element, a filter for selectively transmitting the plurality of wavelengths received from the collection element, and a detection system for measuring intensity of the radiation received from the filter means. A translational stage is used for mounting the optical system and for moving the optical system parallel to the planar array in order to move the collection element back and forth across the lanes in order to receive radiation from the lanes, one lane at a time during electrophoresis. Also included is a computer system for controlling the filter and the stage, and for receiving intensity data from the detector and correlating that data with the corresponding lane and corresponding wavelengths transmitted by the filter in substantially real time.
4812394 FLOW CYTOMERIC MEASUREMENT OF DNA AND INCORPORATED NUCLEOSIDE ANALOGS Frank A Dolbeare, Joe W Gray assigned to University of California A method is provided for simultaneously measuring total cellular DNA and incorporated nucleoside analog. The method entails altering the cellular DNA of cells grown in the presence of a nucleoside analog so that single stranded and double stranded portions are present. Separate stains are used against the two portions. An immunochemical stain is used against the single stranded portion to provide a measure of incorporated nucleoside analog, and a double strand DNA-specific stain is used against the double stranded portion to simultaneously provide a measure of total cellular DNA. The method permits rapid flow cytometric analysis of cell populations, rapid identification of cycling and noncycling subpopulations, and determination of the efficacy of S phase cytotoxic anticancer agents.