- Email: [email protected]
4818680 Method and kit involving displacement and rehybridization of labeled polynucleotide
PATENT
422
group, R2 is hydrogen or a protective group for an amino group, R3 is a carrier having an amino group as the functional group and bound at this functional group in this compound, Y is oxygen atom (-0-) or an imino group (-NH-). This nucleotide, when the X is -CO-(CH2)n-CO-R3, has a carrier (R3) through a spacer (-CO(CH2)m-CO-) on extension from the amino group of deoxycytidine and therefore can be used as the starting material, namely the resin, for synthesizing a 3’-aminated oligonucleotide according to the solid phase method.
4818679 METHOD FOR RECOVERING MUTANT CELLS Lawrence A Chasin, Gail Chasin assigned to The Trustees of Columbia University in the City of New York This invention concerns a method for recovering mutant cells tihich produce a substance in a quantifiably reduced amount relative to wild type cells, and the mutant cells so recovered. The method involves contacting the mutant cells under suitable conditions with a suitable amount of an appropriate detectable compound capable of binding to the substance when it is present in the cells so as to permit the detectable compound to bind to the substance. The amount of the detectable compound bound to the substance in the cells is then detected, and the cells which produce the substance in the quantifiably reduced amount are thereby detected. Such cells which produce the quantifiably reduced amount of the substance are then recovered. This invention is applicable to a wide variety of cell types of various genetic characteristics and should therefore be useful in providing a wide range of useful mutant cells.
4818680 METHOD AND KIT INVOLVING DISPLACEMENT AND REHYBRIDIZATION OF LABELED POLYNLJCLEOTIDE Mary Collins, Joseph Dougherty, Marian S Ellwood, Edward F Fritsch, Kenneth A Jacobs A diagnostic reagent is disclosed which is capable of binding to a target nucleotide sequence which is bound to a labeled polynucleotide in a target binding region which is at
ABSTRACTS
least partially co-extensive with the target binding region in the probe polynucleotide which is capable of binding to the target nucleotide sequence. A method is disclosed in which the reagent is contacted with a sample and with a capturing polynucleotide under conditions such that target nucleotide which may be present in the sample binds to the probe polynucleotide and displaces labeled polynucleotide from the reagent complex, and the capturing polynucleotide binds selectively to the displaced labeled polynucleotide in the region of the labeled polynucleotide that had been bound to the probe polynucleotide. Determination of the displaced nucleotide gives a value which is a function of the presence and concentration of target nucleotide in the sample.
4818681 FAST AND SPECIFIC IMMOBILIZATION OF NUCLEIC ACIDS TO SOLID SUPPORTS Nanibhushan Dattagupta Diagnostics Inc
assigned to Molecular
A process for synthesizing an oligonucleotide comprising linking a nucleoside phosphate to a solid support, through the heterocyclic moiety of the nucleoside, coupling a monoor oligonucleotide to the nucleoside phosphate through its phosphate moiety, in at least one step enzymatically lengthening the monoor oligonucleotide, cleaving the resultant oligonucleotide from the solid supportnucleoside phosphate at the phosphate moiety of the nucleoside, and the separating oligonucleotide. After cleaving and separating the solid support-nucleoside phosphate is recycled for further coupling. Advantageously the solid support-nucleoside phosphate is phosphorylated between separation and recycling.
4818685 METHOD AND KIT FOR DIAGNOSING BLOOM’S SYNDROME Michael Sirover assigned to Temple University of the Commonwealth Systems of Higher Education A method and kit for diagnosing Bloom’s syndrome are provided relying on specific mono-
422
group, R2 is hydrogen or a protective group for an amino group, R3 is a carrier having an amino group as the functional group and bound at this functional group in this compound, Y is oxygen atom (-0-) or an imino group (-NH-). This nucleotide, when the X is -CO-(CH2)n-CO-R3, has a carrier (R3) through a spacer (-CO(CH2)m-CO-) on extension from the amino group of deoxycytidine and therefore can be used as the starting material, namely the resin, for synthesizing a 3’-aminated oligonucleotide according to the solid phase method.
4818679 METHOD FOR RECOVERING MUTANT CELLS Lawrence A Chasin, Gail Chasin assigned to The Trustees of Columbia University in the City of New York This invention concerns a method for recovering mutant cells tihich produce a substance in a quantifiably reduced amount relative to wild type cells, and the mutant cells so recovered. The method involves contacting the mutant cells under suitable conditions with a suitable amount of an appropriate detectable compound capable of binding to the substance when it is present in the cells so as to permit the detectable compound to bind to the substance. The amount of the detectable compound bound to the substance in the cells is then detected, and the cells which produce the substance in the quantifiably reduced amount are thereby detected. Such cells which produce the quantifiably reduced amount of the substance are then recovered. This invention is applicable to a wide variety of cell types of various genetic characteristics and should therefore be useful in providing a wide range of useful mutant cells.
4818680 METHOD AND KIT INVOLVING DISPLACEMENT AND REHYBRIDIZATION OF LABELED POLYNLJCLEOTIDE Mary Collins, Joseph Dougherty, Marian S Ellwood, Edward F Fritsch, Kenneth A Jacobs A diagnostic reagent is disclosed which is capable of binding to a target nucleotide sequence which is bound to a labeled polynucleotide in a target binding region which is at
ABSTRACTS
least partially co-extensive with the target binding region in the probe polynucleotide which is capable of binding to the target nucleotide sequence. A method is disclosed in which the reagent is contacted with a sample and with a capturing polynucleotide under conditions such that target nucleotide which may be present in the sample binds to the probe polynucleotide and displaces labeled polynucleotide from the reagent complex, and the capturing polynucleotide binds selectively to the displaced labeled polynucleotide in the region of the labeled polynucleotide that had been bound to the probe polynucleotide. Determination of the displaced nucleotide gives a value which is a function of the presence and concentration of target nucleotide in the sample.
4818681 FAST AND SPECIFIC IMMOBILIZATION OF NUCLEIC ACIDS TO SOLID SUPPORTS Nanibhushan Dattagupta Diagnostics Inc
assigned to Molecular
A process for synthesizing an oligonucleotide comprising linking a nucleoside phosphate to a solid support, through the heterocyclic moiety of the nucleoside, coupling a monoor oligonucleotide to the nucleoside phosphate through its phosphate moiety, in at least one step enzymatically lengthening the monoor oligonucleotide, cleaving the resultant oligonucleotide from the solid supportnucleoside phosphate at the phosphate moiety of the nucleoside, and the separating oligonucleotide. After cleaving and separating the solid support-nucleoside phosphate is recycled for further coupling. Advantageously the solid support-nucleoside phosphate is phosphorylated between separation and recycling.
4818685 METHOD AND KIT FOR DIAGNOSING BLOOM’S SYNDROME Michael Sirover assigned to Temple University of the Commonwealth Systems of Higher Education A method and kit for diagnosing Bloom’s syndrome are provided relying on specific mono-