4835263 Novel compounds containing an oligonucleotide sequence bonded to an intercalating agent, a process for their synthesis and their use

PATENTABSTRACTS substrate is characterized by amino acid sequence substantially identical to the amino acid sequence of native human erythropoietin wherein the methionine-54 is replaced with leucine. DNA encoding for the EPO-substance and expression vectors incorporating the same are disclosed. Therapeutic compositions and methods for treatment of anemic conditions are described.

4835263 NOVEL COMPOUNDS CONTAINING AN OLIGONUCLEOTIDE SEQUENCE BONDED TO AN INTERCALATING AGENT, A PROCESS FOR THEIR SYNTHESIS AND THEIR USE Thanh T Nguyen, Claud Helene, Ulysse Asseline, Tigy, Prance assigned to Centre National De La Recherche Scientifique The present invention relates to a chemical compound which consists of an oligonucleotide or an oligodeoxynucleotide consisting of a natural or modified chain of nucleotides (sic) to which an intercalating group is fixed via a covalent bond.

581

PH081 promoter and a structural gene located downstream therefrom in a suitable medium until the gene product is formed and recovering the gene product from the culture. Pharmacologically important proteinous materials may be efficiently produced with the use of the abovedescribed novel and potent promoter obtained from yeast which is a eukaryotic microorganism.

4837148 AUTONOMOUS REPLICATION SEQUENCES FOR YEAST STRAINS OF THE GENUS PICHIA James M Cregg assigned to Phillips Petroleum Company Novel autonomous replication sequences which are capable of maintaining plasmids as extrachromosomal elements in host strains of the genus Pichia are provided. In addition, novel constructs including these DNA sequences, as well as transformed organisms therewith are provided. Processes for producing the DNA sequences and constructs of the invention, as well as method for isolating such sequences from any source, are provided.

4837151 4837147 PH081 PROMOTOR OF SACCHAROMYCES CEREVISIAE AND USE THEREOF FOR HETEROLOGOUS GENE EXPRESSION Yasuj Oshima, Hiroyuki Araki, Yoshinobu Kaneko, Takatsuki, Japan assigned to Takeda Chemical Industries Ltd The present invention relates to a DNA fragment having a promoter activity of PH081 gene regulating the production of phosphatase, and which is obtainable from Saccharomyces cerevisiae.; a DNA fragment bearing a structural gene downstream from the above PH081 promoter; a transformant containing a DNA fragment bearing the above PHOSI promoter; a transfonnant containing a DNA fragment bearing the above PH081 promoter and a structural gene downstream Born the PH081 promoter; and a process for obtaining a gene product which includes culturing a transformant containing a DNA fragment bearing the above

LIVE VACCINES COMPRISING TWO MUTATIONS AND FOREIGN ANTIGEN Bruce A Stocker assigned to The Board of Trustees of the Leland Stanford Junior University Stanford University Live vaccines are provided and methods for preparing the live vaccines for protection of a host from a pathogenic microorganism. The vaccines are prepared by introducing at least one modification in a gene involved in at least one, normally at least two, biosynthetic pathways, involving the production of products which are unlikely to be found in the disease susceptible host. The modification results in a gene change which cannot be repaired by a single step e.g. polynucleotide deletions and inversions. Where the aro gene suffers such a change, the resultant auxatrophic mutants require aromatic amino acids, p-aminobenzoic acid and 2,3-dihydroxybenzoic acid or a highly concentrated source of absorbable iron. The auxotrophic mutations have substantially reduced or nonexistent virulence, while retaining the desired immunogenicity to