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4870005 Multilayer analysis element
xviii
New Patents
4869903
prior to the incubation for uniformly d~spensing antigens which may be present in the test sample.
METHOD OF SELECTIVELY INHIBITING HIV Jeffrey Lifson, Michael S McGrath, Hin-Win Yeung, Kuo Hwang assigned to Genelabs Incorporated A method of inhibiting HIV replication in and cellular proliferation of HIV-infected cells. The infected cells are exposed to a single-chain ribosome inactivating protein, at a protein concentration and for an exposure period sufficient to produce a substantial reduction in (a) the level of HIV antigen or reverse transcriptase associated with the infected cells, (b) the ratio of viability of infected/uninfected T cells, and/or (c) the ratio of HIV antigen/cellular antigen in infected macrophages. The method is used to treat HIV infection in humans.
4870005 MULTILAYER ANALYSIS ELEMENT Yutaka Akiyoshi, Asaji Kondo, Masao Kitajima, Saitama, Japan assigned to Fuji Photo Film Co Ltd A dry type muitilayer analysis element comprises at least one porous medium layer comprising a membrane filter, to which an antigen (or antibody) is immobilized, and at least one reagent layer through which a subst,~nce(s) which did not participate in an antigen-antibody reaction can permeate. The multilayer analysis element is effective for assaying components present in body fluids, blood, urine, etc., in a simple manner.
4870003 4870006 SIMULTANEOUS ENZYME IMMUNOASSAY FOR DETECTING ANTIGEN AND/OR ANTIBODY IN HUMANS Kenneth H Kortright, David E Hofheinz, David Allman, Meryl Forman, Song Lee, Paulette E Smariga, Candie S Stoner assigned to Coui.ter Corporation A solid-phase immunoassay is provided for determination of members of an immunological pair such as antigen and/or antibody of a single binding pair in human physiological fluid, wherein a single immunoassay enables simultaneous detection of antigen and/or antibody of a single binding pair in a test sample which may have circulating antigen and/or antibody. The immunoassay is characterized by the addition of an amount of antigen or spike of the binding pair to the test sample prior to incubation of the test sample in the presence of a solid-phase absorbed antibody of the binding pair. The test sample preferably also is subjected to a lysing reagent
ANTIGENIC MATERIAL FOR A CHAGAS' DISEASE DETECTION SYSTEM Elizabeth A Dragon, Stacey Sias assigned to Codon T. Cruzi polypeptide antigens that react with serum from chagasic individuals and does not cross-react with serum from uninfected individuals or individuals infected with related parasites such as Leishmania is described. The DNA from T. Cruzi culture trypomastigotes and epimastigotes coding for antigenic material having a molecular weight of 70 kd is identified, sequenced, and inserted into a cloning vector, which, in turn, is inserted into a host cell line. The expressed polypeptide is immunologically reactive with sera from Chagas' disease infect patients. The cloned gene for the 70 kd polypeptide is expressed and purified and a diagnostic test for Chagas' disease comprising the synthesized polypeptide is described.
New Patents
4869903
prior to the incubation for uniformly d~spensing antigens which may be present in the test sample.
METHOD OF SELECTIVELY INHIBITING HIV Jeffrey Lifson, Michael S McGrath, Hin-Win Yeung, Kuo Hwang assigned to Genelabs Incorporated A method of inhibiting HIV replication in and cellular proliferation of HIV-infected cells. The infected cells are exposed to a single-chain ribosome inactivating protein, at a protein concentration and for an exposure period sufficient to produce a substantial reduction in (a) the level of HIV antigen or reverse transcriptase associated with the infected cells, (b) the ratio of viability of infected/uninfected T cells, and/or (c) the ratio of HIV antigen/cellular antigen in infected macrophages. The method is used to treat HIV infection in humans.
4870005 MULTILAYER ANALYSIS ELEMENT Yutaka Akiyoshi, Asaji Kondo, Masao Kitajima, Saitama, Japan assigned to Fuji Photo Film Co Ltd A dry type muitilayer analysis element comprises at least one porous medium layer comprising a membrane filter, to which an antigen (or antibody) is immobilized, and at least one reagent layer through which a subst,~nce(s) which did not participate in an antigen-antibody reaction can permeate. The multilayer analysis element is effective for assaying components present in body fluids, blood, urine, etc., in a simple manner.
4870003 4870006 SIMULTANEOUS ENZYME IMMUNOASSAY FOR DETECTING ANTIGEN AND/OR ANTIBODY IN HUMANS Kenneth H Kortright, David E Hofheinz, David Allman, Meryl Forman, Song Lee, Paulette E Smariga, Candie S Stoner assigned to Coui.ter Corporation A solid-phase immunoassay is provided for determination of members of an immunological pair such as antigen and/or antibody of a single binding pair in human physiological fluid, wherein a single immunoassay enables simultaneous detection of antigen and/or antibody of a single binding pair in a test sample which may have circulating antigen and/or antibody. The immunoassay is characterized by the addition of an amount of antigen or spike of the binding pair to the test sample prior to incubation of the test sample in the presence of a solid-phase absorbed antibody of the binding pair. The test sample preferably also is subjected to a lysing reagent
ANTIGENIC MATERIAL FOR A CHAGAS' DISEASE DETECTION SYSTEM Elizabeth A Dragon, Stacey Sias assigned to Codon T. Cruzi polypeptide antigens that react with serum from chagasic individuals and does not cross-react with serum from uninfected individuals or individuals infected with related parasites such as Leishmania is described. The DNA from T. Cruzi culture trypomastigotes and epimastigotes coding for antigenic material having a molecular weight of 70 kd is identified, sequenced, and inserted into a cloning vector, which, in turn, is inserted into a host cell line. The expressed polypeptide is immunologically reactive with sera from Chagas' disease infect patients. The cloned gene for the 70 kd polypeptide is expressed and purified and a diagnostic test for Chagas' disease comprising the synthesized polypeptide is described.