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4883761 Pertussis toxin gene: Cloning and expression of protective antigen
PATENT ABSTRACTS
442
4883752
4885165
METHOD FOR PREPARING MONOCLONAL ANTIBODY TO HBSAG
PRODUCTION OF ANTI-VIRAL AND ANTI-BACTERIAL AGENTS IN COMBINATION
Yasuyuki Eda, Toshihiro Maeda, Kiyoto Nishiyama, Akira Tashiro, Kumamoto, Japan assigned to Juridical Foundation the ChemoSero-Therapeutic Research Institute Disclosed is a monoclonal antibody to HBsAg which is prepared by forming hybridomas between human peripheral blood lymphocyte cells, derived from humans having high titers of antiHBsAg, and myeloma cells, cloning the hybridomas and selecting the antibodyproducing clones. The monoclonal antibody can react with all the subtypes of HBsAg and is expeered to be very effective in diagnosis and treatment of diseases due to the viral infection,
Simon Skurkovich This invention provides for administering to donor animals a substance that generates both anti-viral agents, such as interferon, in the blood and anti-bacterial or antitoxic antibodies. This is done at a certain time before recovery of plasma so that both the anti-viral substance (interferon), and anti-bacterial or antitoxic antibodies are substantially maximized. In the case of nonhuman animals the blood is recovered from animals to be slaughtered and plasma containing both the interferon and anti-bacterial (antitoxic/antibodies) is separated, thereby providing a significant quantity of scarce serum inexpensively.
4883761 PERTUSSIS TOXIN GENE: CLONING AND EXPRESSION OF PROTECTIVE ANTIGEN Jerry M Keith, Camille Locht assigned to The United States of America as represented by the Department of Health and Human Services The complete nucleotide sequence of the pertussis toxin gene and the deduced amino acid sequences of the individual subunits have been determined. All five subunits are coded by closely linked cistrons and possibly expressed through a polycistronic mRNA, since a promotor-like structure was found in the 5' flanking region. The order of the cistrons is S1, $2, $3, $4, $5, and $3. All subunits contain signal peptides of variable length. The calculated molecular weights of the mature subunits are 25,024 for S1, 21,924 for $2, 21,873 for $3, 12,058 for $4 and ll,013 for $5. All subunits contain signal peptides of variable length. Subunits $2 and $3 share 70~o amino acid homology and 75~ nucleotide homology. Subunit Sl contains two regions of eight amino acids homologous to analogous regions in the A subunit of both cholera and E. coli heat labile toxins, Functional domains in relation to the primary structure and the development of a safer, new generation vaccine against whooping cough are presented.
4885166 HYBRID
INTERFERONS
Francois Meyer, Albert Hinnen, Andreas Meister, Markus G Grutter, Sefik Alkan, Zswitzerlana assigned to Ciba-Geigy Corporation Novel hybrid interferons are produced which are derived from lymphoblastoid interferons alpha-2 and alpha-3 belonging to the interferon alphaB and alphaD groups, respectively. The novel hybrid interferons possess valuable antiviral and antiproliferative properties.
4885170 ANTIBIOTIC
A80509
Robert L Hamill, Raymond Yao assigned to Eli Lilly and Company New antibiotic A80509, its CI-C7-alkyl ester derivatives and salts are useful antibacterial and anticoccidial agents and improve feed efficiency and growth in animals. Methods of making A80509 by fermentation of, and a biologically pure culture of, Streptomyces mutabilis N R R L 18269 are also provided.
442
4883752
4885165
METHOD FOR PREPARING MONOCLONAL ANTIBODY TO HBSAG
PRODUCTION OF ANTI-VIRAL AND ANTI-BACTERIAL AGENTS IN COMBINATION
Yasuyuki Eda, Toshihiro Maeda, Kiyoto Nishiyama, Akira Tashiro, Kumamoto, Japan assigned to Juridical Foundation the ChemoSero-Therapeutic Research Institute Disclosed is a monoclonal antibody to HBsAg which is prepared by forming hybridomas between human peripheral blood lymphocyte cells, derived from humans having high titers of antiHBsAg, and myeloma cells, cloning the hybridomas and selecting the antibodyproducing clones. The monoclonal antibody can react with all the subtypes of HBsAg and is expeered to be very effective in diagnosis and treatment of diseases due to the viral infection,
Simon Skurkovich This invention provides for administering to donor animals a substance that generates both anti-viral agents, such as interferon, in the blood and anti-bacterial or antitoxic antibodies. This is done at a certain time before recovery of plasma so that both the anti-viral substance (interferon), and anti-bacterial or antitoxic antibodies are substantially maximized. In the case of nonhuman animals the blood is recovered from animals to be slaughtered and plasma containing both the interferon and anti-bacterial (antitoxic/antibodies) is separated, thereby providing a significant quantity of scarce serum inexpensively.
4883761 PERTUSSIS TOXIN GENE: CLONING AND EXPRESSION OF PROTECTIVE ANTIGEN Jerry M Keith, Camille Locht assigned to The United States of America as represented by the Department of Health and Human Services The complete nucleotide sequence of the pertussis toxin gene and the deduced amino acid sequences of the individual subunits have been determined. All five subunits are coded by closely linked cistrons and possibly expressed through a polycistronic mRNA, since a promotor-like structure was found in the 5' flanking region. The order of the cistrons is S1, $2, $3, $4, $5, and $3. All subunits contain signal peptides of variable length. The calculated molecular weights of the mature subunits are 25,024 for S1, 21,924 for $2, 21,873 for $3, 12,058 for $4 and ll,013 for $5. All subunits contain signal peptides of variable length. Subunits $2 and $3 share 70~o amino acid homology and 75~ nucleotide homology. Subunit Sl contains two regions of eight amino acids homologous to analogous regions in the A subunit of both cholera and E. coli heat labile toxins, Functional domains in relation to the primary structure and the development of a safer, new generation vaccine against whooping cough are presented.
4885166 HYBRID
INTERFERONS
Francois Meyer, Albert Hinnen, Andreas Meister, Markus G Grutter, Sefik Alkan, Zswitzerlana assigned to Ciba-Geigy Corporation Novel hybrid interferons are produced which are derived from lymphoblastoid interferons alpha-2 and alpha-3 belonging to the interferon alphaB and alphaD groups, respectively. The novel hybrid interferons possess valuable antiviral and antiproliferative properties.
4885170 ANTIBIOTIC
A80509
Robert L Hamill, Raymond Yao assigned to Eli Lilly and Company New antibiotic A80509, its CI-C7-alkyl ester derivatives and salts are useful antibacterial and anticoccidial agents and improve feed efficiency and growth in animals. Methods of making A80509 by fermentation of, and a biologically pure culture of, Streptomyces mutabilis N R R L 18269 are also provided.