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4906565 Method for the selective detection of microbial nucleotides
PATENT ABSTRACTS
4904602 THIOREDOXIN SHUFFLEASE AND USE THEREOF Vincent P Pigiet, James Rusche, Barbara J Schuster assigned to Repligen Corporation The subject invention concerns a novel enzyme named thioredoxin shutflease, means for preparing the same, and procedures for using thioredoxin shufltease to fold proteins containing disulfide crosslinks. Thioredoxin shufflease is a generic term to define enzymes which have the following characteristics: (a) contain a single reactive thiol group; (b) catalyze the exchange of disulfides in a protein undergoing the refolding process; and (c) are not consumed in the oxidation/refolding process. Specifically exemplified is a thioredoxin shutflease produced from an E. coli thioredoxin gene.
4906561 NUCLEAR ISOLATION MEDIUM AND PROCEDURE FOR SEPARATING CELL NUCLEI Jerry T Thornthwaite Nuclear isolation media and procedures are described for dissociating discrete, nonagglomerated cell nuclei from animal tissue without the need to use enzyme treatments, centrifugation or the like in order to achieve the desired separation. The media facilitates separation and maintains the nuclear membrane intact and in its normal physiological environment. When a DNA-fluorochrome stain is included in the medium an essentially one step combination nuclear isolation and DNA staining procedure is used to measure DNA in tissue cells by flow cytometry. Rapid and consistant results are obtained and multiple sampling of the same tissue or comparison between whole tissues and their single cell isolates are also described.
595
A method for the selective determination of microbial cells, in a sample suspected of containing both microbial and non-microbial cells, is described which involves the selective release and enzymatic inactivation of non-microbial nucleotides, followed by the rapid and specific inhibition of the inactivating enzyme and the release and detection of microbial nucteotides in an appropriate assay, such as a bioluminescent assay.
4906742 ENCODING
ANTIGENS LEPRAE
OF M.
Richard A Young, Barry Bloom, Ronald W Davis assigned to Whitehead Institute for Biomedical Research; Albert Einstein College of Medicine of Yeshiva University a division of Yeshiva University]The Board of Trustees of the Leland Stanford Jr University Genes encoding five immunodeterminant protein antigens of the leprosy parasite Mycobacterium leprae have been isolated. The gene encoding the M. leprae 65kD antigen was sequenced and a lambda gtl I gene sublibrary was constructed with fragments of the gene. Recombinant DNA clones producing specific antigenic determinants were isolated using monoclonal antibodies and the sequences of their insert DNAs were determined with a rapid primer extension method. Amino acid sequences for six different epitopes of the M. leprae protein were elucidated. A peptide containing sequences for one of these epitopes, which is unique to M. leprae, was synthesized and shown to bind the appropriate monoclonal antibody; The approach described here can be used to elucidate rapidly protein epitopes that are recognized by antibodies or T cells. In addition, the wellcharacterized M. leprae antigens can be used in prevention, diagnosis and treatment of leprosy.
4908307 4906565 METHOD FOR THE SELECTIVE DETECTION OF MICROBIAL NUCLEOTIDES John G H M Vossen, Nieuwstadt, Netherlands assigned to Minnesota Mining and Manufacturing Company
HYBRIDIZATION METHOD AND PROBE FOR DETECTING NUCLEIC ACID SEQUENCES Karin D Rodland, Peter J Russell assigned to Rodland Karin D Improved hybridization probe compositions and method for detecting nucleic acids of interest
4904602 THIOREDOXIN SHUFFLEASE AND USE THEREOF Vincent P Pigiet, James Rusche, Barbara J Schuster assigned to Repligen Corporation The subject invention concerns a novel enzyme named thioredoxin shutflease, means for preparing the same, and procedures for using thioredoxin shufltease to fold proteins containing disulfide crosslinks. Thioredoxin shufflease is a generic term to define enzymes which have the following characteristics: (a) contain a single reactive thiol group; (b) catalyze the exchange of disulfides in a protein undergoing the refolding process; and (c) are not consumed in the oxidation/refolding process. Specifically exemplified is a thioredoxin shutflease produced from an E. coli thioredoxin gene.
4906561 NUCLEAR ISOLATION MEDIUM AND PROCEDURE FOR SEPARATING CELL NUCLEI Jerry T Thornthwaite Nuclear isolation media and procedures are described for dissociating discrete, nonagglomerated cell nuclei from animal tissue without the need to use enzyme treatments, centrifugation or the like in order to achieve the desired separation. The media facilitates separation and maintains the nuclear membrane intact and in its normal physiological environment. When a DNA-fluorochrome stain is included in the medium an essentially one step combination nuclear isolation and DNA staining procedure is used to measure DNA in tissue cells by flow cytometry. Rapid and consistant results are obtained and multiple sampling of the same tissue or comparison between whole tissues and their single cell isolates are also described.
595
A method for the selective determination of microbial cells, in a sample suspected of containing both microbial and non-microbial cells, is described which involves the selective release and enzymatic inactivation of non-microbial nucleotides, followed by the rapid and specific inhibition of the inactivating enzyme and the release and detection of microbial nucteotides in an appropriate assay, such as a bioluminescent assay.
4906742 ENCODING
ANTIGENS LEPRAE
OF M.
Richard A Young, Barry Bloom, Ronald W Davis assigned to Whitehead Institute for Biomedical Research; Albert Einstein College of Medicine of Yeshiva University a division of Yeshiva University]The Board of Trustees of the Leland Stanford Jr University Genes encoding five immunodeterminant protein antigens of the leprosy parasite Mycobacterium leprae have been isolated. The gene encoding the M. leprae 65kD antigen was sequenced and a lambda gtl I gene sublibrary was constructed with fragments of the gene. Recombinant DNA clones producing specific antigenic determinants were isolated using monoclonal antibodies and the sequences of their insert DNAs were determined with a rapid primer extension method. Amino acid sequences for six different epitopes of the M. leprae protein were elucidated. A peptide containing sequences for one of these epitopes, which is unique to M. leprae, was synthesized and shown to bind the appropriate monoclonal antibody; The approach described here can be used to elucidate rapidly protein epitopes that are recognized by antibodies or T cells. In addition, the wellcharacterized M. leprae antigens can be used in prevention, diagnosis and treatment of leprosy.
4908307 4906565 METHOD FOR THE SELECTIVE DETECTION OF MICROBIAL NUCLEOTIDES John G H M Vossen, Nieuwstadt, Netherlands assigned to Minnesota Mining and Manufacturing Company
HYBRIDIZATION METHOD AND PROBE FOR DETECTING NUCLEIC ACID SEQUENCES Karin D Rodland, Peter J Russell assigned to Rodland Karin D Improved hybridization probe compositions and method for detecting nucleic acids of interest