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4981793 Biological and chemical method for hydroxylating 4-substituted biphenyls and products obtained therefrom
New Patents
throbacter viscosus ATCC 53594, or any cephalosporin C amidase producing, or potentially producing descendants thereof.
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4981791 RNA STABILIZATION
VECTOR
Marlene Belfort assigned to Health Research Incorporated
4981790 STABLE TAT111 CELL LINES, TAT111 GENE PRODUCTS, AND ASSAY METHODS William A Haseltine, Craig A Rosen, Joseph Sodroski, Wei C Goh assigned to Dana Farber Cancer Institute This invention describes stable tat111 cell lines. It is disclosed that by transfecting a preselected tat111 cell line with a vector containing a sufficient amount of the HTLV-III LTR responsive to tat111 gene products for trans-activation and an enhancer upstream of the tat111 responsive segment, it is possible to express high levels of the tat111 gene products. By including a preselected heterologous gene on this vector, it is also possible to express high levels of a desired gene product. A substantially pure protein comprising 86 amino acids and having an apparent molecular weight of about 14,000 dalton and exhibiting trans-activating activity is also disclosed. This protein and polypeptides having transactivating ability, which is also disclosed, can be used to produce high levels of a desired gene product. A method of detecting the presence of HTLV-III/LAV virus in an individual is also disclosed and comprises the step of: (a) incubating whole blood or lymphocytes from the invididual to be tested with tat111 cell lines of the present invention in a culture medium; and (b) screening for cytopathic effects on the cells is also disclosed. A method of screening for a compound that inhibits trans-activation of the tat111 gene product is also disclosed and comprises the steps of: (1) transacting a tat111 cell line of the present invention with a vector containing a gene that expresses a selectable marker and whose expression is under the control of an HTLV-III LTR; (2) transfecting the same type of tat111 cell lines as in step (1) with the selectable gene chosen in step (1) but under the control of a different regulatory sequence; (3) thereafter, adding a preselected compound to each of the cell lines in increasing concentrations; and (4) measuring the expression of the selectable gene product to determine whether the compound effects the tat111 function without being toxic to the cell.
The present invention provides an RNA stabilization vector including a cloning vehicle, a group I intron inserted into the cloning vehicle and foreign DNA inserted into the intron. The present invention further provides a method for enhancing the production of a gene product by stabilizing the mRNA coding for the gene product using the RNA stabilization vector.
4981793 BIOLOGICAL AND CHEMICAL METHOD FOR HYDROXYLATING 4-SUBSTITUTED BIPHENYLS AND PRODUCTS OBTAINED THEREFROM Bruce F Johnson, Frank Mondello assigned to General Electric Company A method is provided for converting 4substituted biphenyls, such 4hydroxybiphenyl, to the correspondizg 3,4’dihydroxybiphenyl utilizing mutant strains of Alcaligenes eutrophus or Pseudomonas putida. Hydroxylation of the unsubstituted biphenyl ring to the corresponding (1 S-cis)-3-(4hydroxyphenyl)-3 5-cyclohexadiene- 1,2-diol and its conversion to the corresponding triacetate, followed by treatment in base to form 3,4’-dihydroxybiphenyl is also provided.
4981956 DNA SEQUENCES ENCODING BOVINE GROWTH FACTORS Brent R Larsen, Ned Siegel, Claire E Kotts, Michael F McGrath, Sharon D Ogden, Gwen Krivi, John Minnerly assigned to Monsanto Company This invention relates to novel peptides having utility for promotion of growth and/or lactation in animals, to processes and DNA useful in production of such peptides, and to methods utilizing such peptides to promote growth or lactation in animals. In some embodiments, the in-
throbacter viscosus ATCC 53594, or any cephalosporin C amidase producing, or potentially producing descendants thereof.
”
4981791 RNA STABILIZATION
VECTOR
Marlene Belfort assigned to Health Research Incorporated
4981790 STABLE TAT111 CELL LINES, TAT111 GENE PRODUCTS, AND ASSAY METHODS William A Haseltine, Craig A Rosen, Joseph Sodroski, Wei C Goh assigned to Dana Farber Cancer Institute This invention describes stable tat111 cell lines. It is disclosed that by transfecting a preselected tat111 cell line with a vector containing a sufficient amount of the HTLV-III LTR responsive to tat111 gene products for trans-activation and an enhancer upstream of the tat111 responsive segment, it is possible to express high levels of the tat111 gene products. By including a preselected heterologous gene on this vector, it is also possible to express high levels of a desired gene product. A substantially pure protein comprising 86 amino acids and having an apparent molecular weight of about 14,000 dalton and exhibiting trans-activating activity is also disclosed. This protein and polypeptides having transactivating ability, which is also disclosed, can be used to produce high levels of a desired gene product. A method of detecting the presence of HTLV-III/LAV virus in an individual is also disclosed and comprises the step of: (a) incubating whole blood or lymphocytes from the invididual to be tested with tat111 cell lines of the present invention in a culture medium; and (b) screening for cytopathic effects on the cells is also disclosed. A method of screening for a compound that inhibits trans-activation of the tat111 gene product is also disclosed and comprises the steps of: (1) transacting a tat111 cell line of the present invention with a vector containing a gene that expresses a selectable marker and whose expression is under the control of an HTLV-III LTR; (2) transfecting the same type of tat111 cell lines as in step (1) with the selectable gene chosen in step (1) but under the control of a different regulatory sequence; (3) thereafter, adding a preselected compound to each of the cell lines in increasing concentrations; and (4) measuring the expression of the selectable gene product to determine whether the compound effects the tat111 function without being toxic to the cell.
The present invention provides an RNA stabilization vector including a cloning vehicle, a group I intron inserted into the cloning vehicle and foreign DNA inserted into the intron. The present invention further provides a method for enhancing the production of a gene product by stabilizing the mRNA coding for the gene product using the RNA stabilization vector.
4981793 BIOLOGICAL AND CHEMICAL METHOD FOR HYDROXYLATING 4-SUBSTITUTED BIPHENYLS AND PRODUCTS OBTAINED THEREFROM Bruce F Johnson, Frank Mondello assigned to General Electric Company A method is provided for converting 4substituted biphenyls, such 4hydroxybiphenyl, to the correspondizg 3,4’dihydroxybiphenyl utilizing mutant strains of Alcaligenes eutrophus or Pseudomonas putida. Hydroxylation of the unsubstituted biphenyl ring to the corresponding (1 S-cis)-3-(4hydroxyphenyl)-3 5-cyclohexadiene- 1,2-diol and its conversion to the corresponding triacetate, followed by treatment in base to form 3,4’-dihydroxybiphenyl is also provided.
4981956 DNA SEQUENCES ENCODING BOVINE GROWTH FACTORS Brent R Larsen, Ned Siegel, Claire E Kotts, Michael F McGrath, Sharon D Ogden, Gwen Krivi, John Minnerly assigned to Monsanto Company This invention relates to novel peptides having utility for promotion of growth and/or lactation in animals, to processes and DNA useful in production of such peptides, and to methods utilizing such peptides to promote growth or lactation in animals. In some embodiments, the in-