- Email: [email protected]
5002870 Plastin isoforms and their use
447
PATENT ABSTRAC3'S
5001065
5002867
HUMAN CELL LINE AND TRIOMAS, ANTIBODIES, AND TRANSFORMANTS DERIVED
NUCLEIC ACID SEQUENCE D E T E R M I N A T I O N BY M U L T I P L E MIXED OLIGONUCLEOTIDE PROBES
THEREFROM James Larrick, Georg Senyk assigned to Cetus Corporation A stable, continuous human cell line or progeny thereof is produced that is resistant to 6thioguanine and ouabain, secretes less than 40 ng/ml of endogenous lgM antibodies, and grows with a doubling time of about 18 hours. The cell line, which preferably is adapted to serum-free medium, may be used as a fusion partner with an antibody-producing cell line so as to generate antibodies. In addition, it may be electroporated with a vector containing a gene of interest to produce a transformed cell line which generates a protein encoded by the gene, such as an igG or lgM antibody.
Stephen C Macevicz A method is provided for sequencing nucleic acids without the need to separate similarly sized DNAs or RNAs by gel electrophoresis. The method relies on the separate hybridization of multiple mixed oligonucleotide probes to a target sequence. The mixed oligonucleotide probes comprise sequences of fixed and non-fixed bases corresponding to every possible permutation of fixed and non-fixed bases less than or equal to the length of the probes. For each probe, the hybridizations provide the number of times the probe's particular sequence of fixed bases appears in the target sequence. The target sequence is then mathematically reconstructed from this data and a knowledge of the probe sequences.
5001230
5O02868
T CELL ACTIVATION MARKERS
DNA SEQUENCING PROCESS USING STABLE ISOTOPES
Keith D Brown, Timothy K Mosmann, Gerard Zurawski, Sandra M Kurawski, Hunters Hill, Australia assigned to Schering Corporation
K Bruc Jacobson, Harold Schmitt assigned to Atom Sciences Inc
Corinna Herrnstadt, Edward Wilcox assigned to Mycogen Corporation
A DNA sequencing process using specific stable isotopes associated with specific terminators for labels. The process includes a step of incorporating a stable isotope in at least one of the deoxynucleoside triphosphates and/or the dideoxynucleoside triphosphates such that a terminatecl strand has included within it or at the end a stable isotope such as an isotope of sulphur. Replicated strands are then separated by performing gel electrophoresis thereon. The location of the DNA strand with the stable isotope assigned to a terminator is analyzed preferably by resonance ionization spectroscopy. The stable isotopes can be chosen such that specific labels are assigned to at least one, and preferably to each base, in the dideoxynucleoside triphosphates. In the preferred embodiment, each of the bases (A, T, G and C) are associated with a specific stable isotopic label, and can be analyzed in a single track which enhances the accuracy of the ,sequencing process.
The toxin gene encoding a protein toxic to beetles of the order Coleoptera, named M-7, has been cloned and expressed. M-7 is a novel Bacillus thuringiensis strain which has been deposited with a recognized culture repository. The microbe is now known as B. thuringiensis strain san diego.
PLASTIN ISOFORMS AND THEIR USE John C Leavitt, Ching-Shwun Lin, Ruedi Aebersold assigned to California Institute for Medical Research
A polypeptide designated H400 and its encoding nucleic acid are provided as markers specific for activated human T ceils. Activated t cells are detected immunochemically by monoclonal antibodies specific for H400 or its immunogenic peptides. Activated T cells are also detected by nucleic acid probes directed to messenger RNA encoding the H400 protein. 5002765 CLONING AND EXPRESSION OF BACILLUS THURINGIENSIS GENE TOXIC TO BEETLES OF THE ORDER COLEOPTERA
.5002870
448
PATENT ABSTRACTS
A method and reagents are provided for determining whether a human cell is a hemopoietic cell and whether a human tissue cell is in a neoplastic state. Human cells which express only leukocyte-plastin (I-plastin) are hemopoietic cells and human cells which express both Iplastin and tissue-plastin (t-plastin) are neoplastic. The method can be performed using isoform-specific plastin nucleotide probes or isoform-specific antiplastin antibodies.
Sanford A Lacks, Susana Martinez, Paloma Lopez, Manuel Espinosa assigned to The United States of America as represented by the United States Department of Energy A method is disclosed for cloning the gene which encodes a DNA polymerase-exonuclease of Streptococcus pneumoniae. Plasmid pSM22, the vector containing the pneumocccal polA gene, facilitates the expression of 50-fold greater amounts of the Poll enzyme.
5OO2873
5002876
DNA SEQUENCE ENCODING A LYMPHOCYTE ADHESION RECEPTOR FOR HIGH ENDOTHELIUM
YEAST PRODUCTION OF HUMAN TUMOR NECROSIS FACTOR
John Thomas St, W Michael Gallatin, Rejean L Idzerda assigned to Fred Hutchinson Cancer Research Center A lymphoid cell line eDNA that encodes an adhesion receptor for high endothelial venules (HEV).
5002874 GENETICALLY ENGINEERED EUCARYOTIC HOST CELLS CAPABLE OF EXPRESSING MODIFIED FORMS OF EIF-2 ALPHA Randal J Kaufman assigned to Genetics Institute Inc Eucaryotic host cells are disclosed which contain a DNA molecule encoding an elF-2 alpha mutant and preferably a DNA sequence encoding a desired heterologous protein. The DNA sequences are linked to expression control sequences permitting expression of the mutant elF-2 alpha gene and the heterologous gene. Culturing such cells provides a method for the production of the desired heterologous protein. The mutations eliminate one or both serine residues at positions 48 and 51 of the elF-2 sequence. In another aspect of the invention, the elF-2 5"untranslated sequence was observed to have effects on translation of heterologous mRNAs.
5002875 PLASIMIDS CONTAINING THE GENE FOR DNA POLYMERASE I FROM STREPTOCOCCUS PNEUMONIAE
Kotikanyadan Sreekrishna, Motohiro Euke, Rica H Potenz assigned to Phillips Petroleum Company Novel DNA constructs comprising yeast regulatory regions plus the structural coding region for human tumor necrosis factor, are disclosed. These novel constructs are incorporated into a variety of linear and circular plasmids. Such plasmids are used for yeast transformation and ultimately for the production of human tumor necrosis factor by yeast.
5002882 METHOD FOR PRODUCING THE XMA! RESTRICTION ENDONUCLEASE AND METHYLASE Keith D Lunnen, Geoffrey G Wilson assigned to New England Biolabs Inc The present invention is directed to a method for cloning and producing the Xmal restriction endonuclease by (1) introducing the restriction endonuclease gene from X. malvacaerum into a host whereby the restriction gene is expressed: (2) fermenting the host which contains the plasmid encoding and expressing the Xmal restriction endonuclease activity, and (3) purifying the Xmal restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the Xmal restriction endonuclease activity. 5O02888 MUTANT MICROORGANISMS U S E F U L F O R CLEAVAGE O F O R G A N I C C-S B O N D S John J Kilbane assigned to Institute of Gas Technology
PATENT ABSTRAC3'S
5001065
5002867
HUMAN CELL LINE AND TRIOMAS, ANTIBODIES, AND TRANSFORMANTS DERIVED
NUCLEIC ACID SEQUENCE D E T E R M I N A T I O N BY M U L T I P L E MIXED OLIGONUCLEOTIDE PROBES
THEREFROM James Larrick, Georg Senyk assigned to Cetus Corporation A stable, continuous human cell line or progeny thereof is produced that is resistant to 6thioguanine and ouabain, secretes less than 40 ng/ml of endogenous lgM antibodies, and grows with a doubling time of about 18 hours. The cell line, which preferably is adapted to serum-free medium, may be used as a fusion partner with an antibody-producing cell line so as to generate antibodies. In addition, it may be electroporated with a vector containing a gene of interest to produce a transformed cell line which generates a protein encoded by the gene, such as an igG or lgM antibody.
Stephen C Macevicz A method is provided for sequencing nucleic acids without the need to separate similarly sized DNAs or RNAs by gel electrophoresis. The method relies on the separate hybridization of multiple mixed oligonucleotide probes to a target sequence. The mixed oligonucleotide probes comprise sequences of fixed and non-fixed bases corresponding to every possible permutation of fixed and non-fixed bases less than or equal to the length of the probes. For each probe, the hybridizations provide the number of times the probe's particular sequence of fixed bases appears in the target sequence. The target sequence is then mathematically reconstructed from this data and a knowledge of the probe sequences.
5001230
5O02868
T CELL ACTIVATION MARKERS
DNA SEQUENCING PROCESS USING STABLE ISOTOPES
Keith D Brown, Timothy K Mosmann, Gerard Zurawski, Sandra M Kurawski, Hunters Hill, Australia assigned to Schering Corporation
K Bruc Jacobson, Harold Schmitt assigned to Atom Sciences Inc
Corinna Herrnstadt, Edward Wilcox assigned to Mycogen Corporation
A DNA sequencing process using specific stable isotopes associated with specific terminators for labels. The process includes a step of incorporating a stable isotope in at least one of the deoxynucleoside triphosphates and/or the dideoxynucleoside triphosphates such that a terminatecl strand has included within it or at the end a stable isotope such as an isotope of sulphur. Replicated strands are then separated by performing gel electrophoresis thereon. The location of the DNA strand with the stable isotope assigned to a terminator is analyzed preferably by resonance ionization spectroscopy. The stable isotopes can be chosen such that specific labels are assigned to at least one, and preferably to each base, in the dideoxynucleoside triphosphates. In the preferred embodiment, each of the bases (A, T, G and C) are associated with a specific stable isotopic label, and can be analyzed in a single track which enhances the accuracy of the ,sequencing process.
The toxin gene encoding a protein toxic to beetles of the order Coleoptera, named M-7, has been cloned and expressed. M-7 is a novel Bacillus thuringiensis strain which has been deposited with a recognized culture repository. The microbe is now known as B. thuringiensis strain san diego.
PLASTIN ISOFORMS AND THEIR USE John C Leavitt, Ching-Shwun Lin, Ruedi Aebersold assigned to California Institute for Medical Research
A polypeptide designated H400 and its encoding nucleic acid are provided as markers specific for activated human T ceils. Activated t cells are detected immunochemically by monoclonal antibodies specific for H400 or its immunogenic peptides. Activated T cells are also detected by nucleic acid probes directed to messenger RNA encoding the H400 protein. 5002765 CLONING AND EXPRESSION OF BACILLUS THURINGIENSIS GENE TOXIC TO BEETLES OF THE ORDER COLEOPTERA
.5002870
448
PATENT ABSTRACTS
A method and reagents are provided for determining whether a human cell is a hemopoietic cell and whether a human tissue cell is in a neoplastic state. Human cells which express only leukocyte-plastin (I-plastin) are hemopoietic cells and human cells which express both Iplastin and tissue-plastin (t-plastin) are neoplastic. The method can be performed using isoform-specific plastin nucleotide probes or isoform-specific antiplastin antibodies.
Sanford A Lacks, Susana Martinez, Paloma Lopez, Manuel Espinosa assigned to The United States of America as represented by the United States Department of Energy A method is disclosed for cloning the gene which encodes a DNA polymerase-exonuclease of Streptococcus pneumoniae. Plasmid pSM22, the vector containing the pneumocccal polA gene, facilitates the expression of 50-fold greater amounts of the Poll enzyme.
5OO2873
5002876
DNA SEQUENCE ENCODING A LYMPHOCYTE ADHESION RECEPTOR FOR HIGH ENDOTHELIUM
YEAST PRODUCTION OF HUMAN TUMOR NECROSIS FACTOR
John Thomas St, W Michael Gallatin, Rejean L Idzerda assigned to Fred Hutchinson Cancer Research Center A lymphoid cell line eDNA that encodes an adhesion receptor for high endothelial venules (HEV).
5002874 GENETICALLY ENGINEERED EUCARYOTIC HOST CELLS CAPABLE OF EXPRESSING MODIFIED FORMS OF EIF-2 ALPHA Randal J Kaufman assigned to Genetics Institute Inc Eucaryotic host cells are disclosed which contain a DNA molecule encoding an elF-2 alpha mutant and preferably a DNA sequence encoding a desired heterologous protein. The DNA sequences are linked to expression control sequences permitting expression of the mutant elF-2 alpha gene and the heterologous gene. Culturing such cells provides a method for the production of the desired heterologous protein. The mutations eliminate one or both serine residues at positions 48 and 51 of the elF-2 sequence. In another aspect of the invention, the elF-2 5"untranslated sequence was observed to have effects on translation of heterologous mRNAs.
5002875 PLASIMIDS CONTAINING THE GENE FOR DNA POLYMERASE I FROM STREPTOCOCCUS PNEUMONIAE
Kotikanyadan Sreekrishna, Motohiro Euke, Rica H Potenz assigned to Phillips Petroleum Company Novel DNA constructs comprising yeast regulatory regions plus the structural coding region for human tumor necrosis factor, are disclosed. These novel constructs are incorporated into a variety of linear and circular plasmids. Such plasmids are used for yeast transformation and ultimately for the production of human tumor necrosis factor by yeast.
5002882 METHOD FOR PRODUCING THE XMA! RESTRICTION ENDONUCLEASE AND METHYLASE Keith D Lunnen, Geoffrey G Wilson assigned to New England Biolabs Inc The present invention is directed to a method for cloning and producing the Xmal restriction endonuclease by (1) introducing the restriction endonuclease gene from X. malvacaerum into a host whereby the restriction gene is expressed: (2) fermenting the host which contains the plasmid encoding and expressing the Xmal restriction endonuclease activity, and (3) purifying the Xmal restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the Xmal restriction endonuclease activity. 5O02888 MUTANT MICROORGANISMS U S E F U L F O R CLEAVAGE O F O R G A N I C C-S B O N D S John J Kilbane assigned to Institute of Gas Technology