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5024933 Method and kit for sample adherence to test substrate
PATENT ABSTRACTS
490
substituted, ortho-fused pyrazine fluorophore, with neither nitrogen of the pyrazine ring being fused or substituted, is produced by enzymatic oxidation of a fluorophore precursor substrate which comprises a nitrogen-substituted, cyclic compound. Also provided are novel fluorophore-labelled compounds.
5017501 PREPARATION OF UNIFORMLY SIZED LIPOSOMES ENCAPSULATING AN AQUEOUS LIQUID Martin Wong assigned to Abbott Laboratories
5017489 CYTOTOXIC T LYMPHOCTE SERINE ESTERASE AND METHOD FOR STIMULATION AND INHIBITION Mark S Pasternack, Herman S Eisen assigned to Massachusetts Institute of Technology Antibodies, nucleic acid sequences, and methods for inhibition of lysis for a novel serine esterase produced by both murine and human cytotoxic T lymphocytes. The serine esterase has an apparent molecular weight of approximately 28,000-31,000, as determined by SDS gel clectrophoresis under reducing conditions, and trypsin-like activity. Inhibition of the esterase correlates with inhibition of the cells" cytolytic activity. Specific inhibition of the serine esterase is useful as a method for immunosuppression as well as for the inhibition ofcytolytic activity o f t lymphocytes, both in vivo and in vitro. The genes encoding the murine and human serine esterase are homologous.
A dispersion of a uniformly sized population of multilamellar lipid vesicles (liposomes) encapsulating an aqueous liquid is prepared by forming a dried film oflipids on the walls of a vessel, contacting the film with an aqueous liquid in the presence of spherical contact masses such as glass beads and agitating. The liposomes have mean diameters in the range of about 150 to about 3000 nanometersand the contact masses have mean diameters of about 50 to 3,000 microns. The aqueous liquid encapsulated may contain enzymes, drugs or marker substances such as eolorimetric or fluorescent dyes. A member of a immunological binding pair may be asstriated with the surfaces of the liposomes for carrying out immunoassays. This method allows for the use of small quantities of marker and lipid, leaves no residual solvents, allows Ibr contact with only glass surfaces and involves no transfer ofliposome preparations from lipid film drying vessels to sizing apparatus.
5024933 5017494 BIO-THERMO TIP S E N S O R
METHOD AND KIT FOR SAMPLE ADHERENCE TO TEST SUBSTRATE
Isao Karube, Hiroshi Muramatsu, Tachikawa, Japan assigned to Seiko Instruments & Electronics Ltd
Huey-Lang Yang, John "l'odd, Huey-Ling L Jou assigned to Enzo Biochcm Inc
A bio-thermo sensor has an enzyme-modified membrane directly supported on an IC thermal detector in face-to-face contact. The enzymemodified membrane selectively reacts with a specific chemical substance to generate therein a reaction heat which is converted into an amplified output voltage indicative of the chemical substance by the IC thermal detector. The IC thermal detector includes Darlington-connected transistors which provide high sensitivity and quick response time.
Disclosed is an improvement relating to adhesion of samples onto a surface for nucleic acid hybridization assay to detect a target polynucleotide. In one aspect, the invention provides a method of adhering a tissue, cell or other target polynucleotide-containing sample to a substrate under nucleic acid hybridization assay compatible conditions. In another aspect, the method comprises a hybridization assay procedure. Also disclosed are kits for performance of such procedures.
490
substituted, ortho-fused pyrazine fluorophore, with neither nitrogen of the pyrazine ring being fused or substituted, is produced by enzymatic oxidation of a fluorophore precursor substrate which comprises a nitrogen-substituted, cyclic compound. Also provided are novel fluorophore-labelled compounds.
5017501 PREPARATION OF UNIFORMLY SIZED LIPOSOMES ENCAPSULATING AN AQUEOUS LIQUID Martin Wong assigned to Abbott Laboratories
5017489 CYTOTOXIC T LYMPHOCTE SERINE ESTERASE AND METHOD FOR STIMULATION AND INHIBITION Mark S Pasternack, Herman S Eisen assigned to Massachusetts Institute of Technology Antibodies, nucleic acid sequences, and methods for inhibition of lysis for a novel serine esterase produced by both murine and human cytotoxic T lymphocytes. The serine esterase has an apparent molecular weight of approximately 28,000-31,000, as determined by SDS gel clectrophoresis under reducing conditions, and trypsin-like activity. Inhibition of the esterase correlates with inhibition of the cells" cytolytic activity. Specific inhibition of the serine esterase is useful as a method for immunosuppression as well as for the inhibition ofcytolytic activity o f t lymphocytes, both in vivo and in vitro. The genes encoding the murine and human serine esterase are homologous.
A dispersion of a uniformly sized population of multilamellar lipid vesicles (liposomes) encapsulating an aqueous liquid is prepared by forming a dried film oflipids on the walls of a vessel, contacting the film with an aqueous liquid in the presence of spherical contact masses such as glass beads and agitating. The liposomes have mean diameters in the range of about 150 to about 3000 nanometersand the contact masses have mean diameters of about 50 to 3,000 microns. The aqueous liquid encapsulated may contain enzymes, drugs or marker substances such as eolorimetric or fluorescent dyes. A member of a immunological binding pair may be asstriated with the surfaces of the liposomes for carrying out immunoassays. This method allows for the use of small quantities of marker and lipid, leaves no residual solvents, allows Ibr contact with only glass surfaces and involves no transfer ofliposome preparations from lipid film drying vessels to sizing apparatus.
5024933 5017494 BIO-THERMO TIP S E N S O R
METHOD AND KIT FOR SAMPLE ADHERENCE TO TEST SUBSTRATE
Isao Karube, Hiroshi Muramatsu, Tachikawa, Japan assigned to Seiko Instruments & Electronics Ltd
Huey-Lang Yang, John "l'odd, Huey-Ling L Jou assigned to Enzo Biochcm Inc
A bio-thermo sensor has an enzyme-modified membrane directly supported on an IC thermal detector in face-to-face contact. The enzymemodified membrane selectively reacts with a specific chemical substance to generate therein a reaction heat which is converted into an amplified output voltage indicative of the chemical substance by the IC thermal detector. The IC thermal detector includes Darlington-connected transistors which provide high sensitivity and quick response time.
Disclosed is an improvement relating to adhesion of samples onto a surface for nucleic acid hybridization assay to detect a target polynucleotide. In one aspect, the invention provides a method of adhering a tissue, cell or other target polynucleotide-containing sample to a substrate under nucleic acid hybridization assay compatible conditions. In another aspect, the method comprises a hybridization assay procedure. Also disclosed are kits for performance of such procedures.