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5084566 DNA probe which reveals a hypervariable region on human chromosone 1
276
PATENTABSTRACTS
5084383
5084385
BACILLUS SUBTILIS STRAIN WHOSE EXTRACELLULAR PROTEASE ACTIVITIES ARE REDUCED, METHOD FOR OBTAINING THE STRAIN AND METHOD FOR SECRETING P R O T E I N S BY U S I N G T H E STRAIN
PROCESS FOR PRODUCING ALCOHOL USING YEAST T R A N S F O R M E D BY R H I Z O P U S GLUCOAMYLASE GENE
Yoshio Furutani, Masaru Honjo, Akira Nakayama, Koichi Kawamura, Hiroaki Shimada, Izumi Mita, Akiko Akaoka, Kanagawa, Japan assigned to Agency of Industrial Science and Technology; Ministry of International Trade and Indust Residual extracellular protease activities of a Bacillus subtilis strain can be further reduced by introducing a gene for the stimulation of extracellular protease levels into the genomic DNA of the strain. The resultant strain whose extracellular protease activities are further reduced is transformed with a recombinant plasmid for secretion of a desired protein and the desired protein can be efficiently produced and accumulated in the culture medium in a large amount. The accumulated desired protein can be easily recovered from the culture medium. For example, the accumulation level of human growth hormone secreted by a B. subtilis MT430 strain carrying recombinant DNA phGH427 for the expression and secretion of human growth hormone can be increased five to tenfold as a result of the above treatment for reduction of the residual extracellular protease activities of the extracellular protease-activitydeficient strain.
Toshihiko Ashikari, Norihisa Nakamura, Yoshikazu Tanaka, Yuji Shibano, Hajime Yoshizumi, Osaka, Japan assigned to Suntory Limited This invention provides a process for producing ethanol from a starchy material, wherein the starchy material is simultaneously subjected to saccharification and subsequent fermentation by use of a yeast host which has been transformed by an expressible recombinant vector comprising a glucoamylase gene derived from a fungus of the genus Rhizopus.
~84~9 PROTEIN
A DOMAIN MUTANTS
Albert T Profy assigned to Repligen Corporation The subject invention concerns novel protein A or protein A-like molecules that can be coupled to other materials through a single, defined site on the protein A molecule. Specifically exemplified is Cysteinyl-rProtein A trademark. The compounds of the invention, for example, Cysteinyl-rProtein A trademark, can be used in processes wherein protein A is used.
50~5~ 5084384 SECRETION OF INSULIN-LIKE GROWTH FACTOR-I Edith Wong, Michael L Bittner assigned to Monsanto Company A method for bacterially producing IGF-I is disclosed in which Gram-negative bacteria are caused to express a gene consisting of a lamb or ompF signal sequence operatively joined to a DNA sequence encoding IGF-I and producing IGF-I which is secreted into the periplasmic space of the bacteria.
DNA PROBE WHICH REVEALS A HYPERVARIABLE REGION ON HUMAN CHROMOSONE 1 Michae Litt, Norman Buroker A DNA probe pl-79 is homologous to at least a portion of a hypervariable DNA region located at chromosome 1p36.3 in the human genome. The DNA region displays extensive restriction fragment length polymorphisms when digested with certain restriction endonucleases. Probe pl79 is believed to have a repeated 39 base sequence CCTGGGGGTGNGNGTGCTG TTCCAG-
PATENTABSTRACTS GCTGTCAGAGGCTC, and can be used as a genetic fingerprint to establish human identity, determine engraftment of bone marrow transplants, determine parentage, and otherwise map genes.
277
5085982 METHOD OF DETECTING S P E C I F I C S U B S T A N C E S BY SELECTIVE GROWTH OF LIVING CELLS Douglas H Keith assigned to City of Hope
5085588 BACTERIAL PROMOTERS I N D U C I B L E BY P L A N T EXTRACTS Sharon Long, John T Mulligan, Thomas Egelhoff Novel constructs are provided containing DNA fragments comprising a Rhizobium nodulation gene divergent transcriptional initiation regulatory region. The region is responsive to plant exudate in the presence of a nod D gene product. When associated with plants, the region can control the expression of structural genes, such as agents active in protecting plants or inducing their growth. This invention was funded at least in-part by a grant from the National Institutes of Health. The U.S. government may have certain rights in this invention.
5085862 GENETIC DETOXIFICATION PERTUSSIS TOXIN
OF
The invented method utilizes a signal amplification system comprising living cells which are specifically provided with the ability to survive, reproduce and be detected in the event that a target molecule is present. The method comprises first the step of binding a target molecule to a substratum. In the second step a phagemid is prepared which is capable of transfecting a cell enabling such transfected cell to produce a signal, such as color or light. The phagemid is then provided with a means for binding to a probe, thus forming a phagemid complex. The probe may also be provided with a means of binding to the phagemid complex. This modified probe is then hybridized with the target molecules bound to the substratum. Thereafter, the phagemid complex is hybridized with the modified probe and specific binding occurs between the phagemid complex and the modified probe thus forming a substrutum-target-probe phagemid complex. As a next step, lysogenic bacterial cells which are infectable by the particular phagemid being used are added to the system in an environment which favors transfection of the phagemid nucleic acid into the bacteria. The growth medium is constituted so that only transfected bacteria survive and are capable of reproducing. The phagemid also enable the bacteria to produce a signal such light or color. Only the transfected bacterial cells containing the plasmid gene for producing the signal will be detectable.
Michel H Klein, Heather A Boux, Stephen A Cockle, Sheena M Loosmore, Gavin R Zealey, Willowdale, Canada assigned to Connaught Laboratories Limited A new method is described for the preparation of a safe, immunogenic and efficacious vaccine for protection against the disease pertussis. In development of this vaccine, specific functional sites of pertussis toxin have been identified, and using this information, defined mutant holotoxins have been produced by site directed mutagenesis of the toxin gene. A number of these toxin analogues are detoxified, retain an immunodominant S1 epitope, are immunoganic and are protective in the standard pertussis vaccine potency test in mice.
5085983 DETECTION OF HUMAN TUMOR PROGRESSION AND DRUG RESISTANCE Kevin J Scanlon assigned to City of Hope Changes in tumor cell RNA and DNA are utilized to detect the progression and the temporal changes in resistance to chemotherapy in human tumors.
PATENTABSTRACTS
5084383
5084385
BACILLUS SUBTILIS STRAIN WHOSE EXTRACELLULAR PROTEASE ACTIVITIES ARE REDUCED, METHOD FOR OBTAINING THE STRAIN AND METHOD FOR SECRETING P R O T E I N S BY U S I N G T H E STRAIN
PROCESS FOR PRODUCING ALCOHOL USING YEAST T R A N S F O R M E D BY R H I Z O P U S GLUCOAMYLASE GENE
Yoshio Furutani, Masaru Honjo, Akira Nakayama, Koichi Kawamura, Hiroaki Shimada, Izumi Mita, Akiko Akaoka, Kanagawa, Japan assigned to Agency of Industrial Science and Technology; Ministry of International Trade and Indust Residual extracellular protease activities of a Bacillus subtilis strain can be further reduced by introducing a gene for the stimulation of extracellular protease levels into the genomic DNA of the strain. The resultant strain whose extracellular protease activities are further reduced is transformed with a recombinant plasmid for secretion of a desired protein and the desired protein can be efficiently produced and accumulated in the culture medium in a large amount. The accumulated desired protein can be easily recovered from the culture medium. For example, the accumulation level of human growth hormone secreted by a B. subtilis MT430 strain carrying recombinant DNA phGH427 for the expression and secretion of human growth hormone can be increased five to tenfold as a result of the above treatment for reduction of the residual extracellular protease activities of the extracellular protease-activitydeficient strain.
Toshihiko Ashikari, Norihisa Nakamura, Yoshikazu Tanaka, Yuji Shibano, Hajime Yoshizumi, Osaka, Japan assigned to Suntory Limited This invention provides a process for producing ethanol from a starchy material, wherein the starchy material is simultaneously subjected to saccharification and subsequent fermentation by use of a yeast host which has been transformed by an expressible recombinant vector comprising a glucoamylase gene derived from a fungus of the genus Rhizopus.
~84~9 PROTEIN
A DOMAIN MUTANTS
Albert T Profy assigned to Repligen Corporation The subject invention concerns novel protein A or protein A-like molecules that can be coupled to other materials through a single, defined site on the protein A molecule. Specifically exemplified is Cysteinyl-rProtein A trademark. The compounds of the invention, for example, Cysteinyl-rProtein A trademark, can be used in processes wherein protein A is used.
50~5~ 5084384 SECRETION OF INSULIN-LIKE GROWTH FACTOR-I Edith Wong, Michael L Bittner assigned to Monsanto Company A method for bacterially producing IGF-I is disclosed in which Gram-negative bacteria are caused to express a gene consisting of a lamb or ompF signal sequence operatively joined to a DNA sequence encoding IGF-I and producing IGF-I which is secreted into the periplasmic space of the bacteria.
DNA PROBE WHICH REVEALS A HYPERVARIABLE REGION ON HUMAN CHROMOSONE 1 Michae Litt, Norman Buroker A DNA probe pl-79 is homologous to at least a portion of a hypervariable DNA region located at chromosome 1p36.3 in the human genome. The DNA region displays extensive restriction fragment length polymorphisms when digested with certain restriction endonucleases. Probe pl79 is believed to have a repeated 39 base sequence CCTGGGGGTGNGNGTGCTG TTCCAG-
PATENTABSTRACTS GCTGTCAGAGGCTC, and can be used as a genetic fingerprint to establish human identity, determine engraftment of bone marrow transplants, determine parentage, and otherwise map genes.
277
5085982 METHOD OF DETECTING S P E C I F I C S U B S T A N C E S BY SELECTIVE GROWTH OF LIVING CELLS Douglas H Keith assigned to City of Hope
5085588 BACTERIAL PROMOTERS I N D U C I B L E BY P L A N T EXTRACTS Sharon Long, John T Mulligan, Thomas Egelhoff Novel constructs are provided containing DNA fragments comprising a Rhizobium nodulation gene divergent transcriptional initiation regulatory region. The region is responsive to plant exudate in the presence of a nod D gene product. When associated with plants, the region can control the expression of structural genes, such as agents active in protecting plants or inducing their growth. This invention was funded at least in-part by a grant from the National Institutes of Health. The U.S. government may have certain rights in this invention.
5085862 GENETIC DETOXIFICATION PERTUSSIS TOXIN
OF
The invented method utilizes a signal amplification system comprising living cells which are specifically provided with the ability to survive, reproduce and be detected in the event that a target molecule is present. The method comprises first the step of binding a target molecule to a substratum. In the second step a phagemid is prepared which is capable of transfecting a cell enabling such transfected cell to produce a signal, such as color or light. The phagemid is then provided with a means for binding to a probe, thus forming a phagemid complex. The probe may also be provided with a means of binding to the phagemid complex. This modified probe is then hybridized with the target molecules bound to the substratum. Thereafter, the phagemid complex is hybridized with the modified probe and specific binding occurs between the phagemid complex and the modified probe thus forming a substrutum-target-probe phagemid complex. As a next step, lysogenic bacterial cells which are infectable by the particular phagemid being used are added to the system in an environment which favors transfection of the phagemid nucleic acid into the bacteria. The growth medium is constituted so that only transfected bacteria survive and are capable of reproducing. The phagemid also enable the bacteria to produce a signal such light or color. Only the transfected bacterial cells containing the plasmid gene for producing the signal will be detectable.
Michel H Klein, Heather A Boux, Stephen A Cockle, Sheena M Loosmore, Gavin R Zealey, Willowdale, Canada assigned to Connaught Laboratories Limited A new method is described for the preparation of a safe, immunogenic and efficacious vaccine for protection against the disease pertussis. In development of this vaccine, specific functional sites of pertussis toxin have been identified, and using this information, defined mutant holotoxins have been produced by site directed mutagenesis of the toxin gene. A number of these toxin analogues are detoxified, retain an immunodominant S1 epitope, are immunoganic and are protective in the standard pertussis vaccine potency test in mice.
5085983 DETECTION OF HUMAN TUMOR PROGRESSION AND DRUG RESISTANCE Kevin J Scanlon assigned to City of Hope Changes in tumor cell RNA and DNA are utilized to detect the progression and the temporal changes in resistance to chemotherapy in human tumors.