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5118668 Variants of bovine pancreatic trypsin inhibitor and pharmaceutical use thereof
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PATENT ABSTRACTS more stable enzyme which retains activity in the presence of hypochlorite. The invention is particularly applicable to alkaline proteases which are useful in detergent compositions.
5118625 AUTONOMOUSLY REPLICATING PLASMID AND SACCHAROMYCOPSIS LIPOLYTICA TRANSFORMED THEREWITH Shuichi Aiba, Tomohis Nagasaki, Kohji Uchida, Saitama, Japan assigned to Oriental Yeast Co Ltd; Nisshin Flour Milling Co Ltd The plasmid of the present invention contains Leu 2 gene ( beta-isopropylmaleate dehydrogenase gene) from Saccharomycopsis lipolytica and an autonomously replicating sequence (hereafter referred to as ARS) and can be a shuttle vector between Escherichia coil and Saccharomycopsis lipolytica since it is produced from shuttle vector pVC 1 of Escherichia coli and Saccharomyces cerevisiae. An exogenous useful gene is inserted into the plasmid of the present invention, namely, shuttle vector to transform Escherichia coli, whereby a large quantity of plasmids are obtained. It becomes possible to produce a useful substance in Saccharomycopsis lipolytica as a host using the plasmid.
moter/terminator vector in a single step allows for conversion of the shuttle vector into a lytic vector of an exogenous gene. The presence of restriction endonuclease enzyme recognition sites facilitative of insertion of a viral functional late gene into the late gene promoter/terminator vector in a single step allows for conversion of the shuttle vector into a lytic vector.
5118668 VARIANTS OF BOVINE PANCREATIC TRYPSIN INHIBITOR AND PHARMACEUTICAL USE THEREOF Ernst-August Auerswald, Wolfgang Bruns, Dietrich Horlein, Gerd Reinhardt, Eugen Schnabel, Werner Schroder, Munich, Federal Republic Of Germany assigned to Bayer Aktiengesellschaft Peptides having essentially the sequence of bovine pancreatic trypsin inhibitor (aprotinin) wherein one or more of the amino acids at positions 15, 16, 17, 18, 34, 39 and 52 are replaced by any naturally occurring amino acid produced by recombinant DNA technology, process, expression vector and recominant host therefor and pharmaceutical use thereof. Such peptides being useful as therapeutic agents in diseases connected with the presence of excessive amounts of proteinases.
5118627 PAPOVA VIRUS CONSTRUCTION Jeffrey K Browne assigned to Amgen A microbial shuttle vector is disclosed which is independently replicative in bacterial cells and mammalian cells and includes in its DNA sequence bacterial plasmid sequences allowing selection and replication in bacterial cells, an SV40 viral origin of replication, and either an SV40 functional early gene promoter and functional early gene terminator or an SV40 functional late gene promoter and functional late gene terminator, the vector having a unique restriction endonuclease enzyme recognition site between the promoter and terminator for insertion of an exogenous gene. The presence of restriction endonuclease enzyme recognition sites facilitative of insertion of a viral functional late gene into the early gene pro-
5118792 ICE CRYSTAL GROWTH SUPPRESSION POLYPEPTIDES AND METHOD OF MAKING Gareth J Warren, Gunhild M Mueller, Robert McKown assigned to DNA Plant Technology Corporation Novel methods of improving freezing tolerance of organic materials through the use of antifreeze polypeptides is provided. These polypeptides increase the storage life of foodstuffs and biologics, as well as protect plant products, such as during growth. The antifreeze polypeptides, or their fusion proteins, may be produced chemically or by recombinant DNA techniques, and then purified for a variety of uses.
PATENT ABSTRACTS more stable enzyme which retains activity in the presence of hypochlorite. The invention is particularly applicable to alkaline proteases which are useful in detergent compositions.
5118625 AUTONOMOUSLY REPLICATING PLASMID AND SACCHAROMYCOPSIS LIPOLYTICA TRANSFORMED THEREWITH Shuichi Aiba, Tomohis Nagasaki, Kohji Uchida, Saitama, Japan assigned to Oriental Yeast Co Ltd; Nisshin Flour Milling Co Ltd The plasmid of the present invention contains Leu 2 gene ( beta-isopropylmaleate dehydrogenase gene) from Saccharomycopsis lipolytica and an autonomously replicating sequence (hereafter referred to as ARS) and can be a shuttle vector between Escherichia coil and Saccharomycopsis lipolytica since it is produced from shuttle vector pVC 1 of Escherichia coli and Saccharomyces cerevisiae. An exogenous useful gene is inserted into the plasmid of the present invention, namely, shuttle vector to transform Escherichia coli, whereby a large quantity of plasmids are obtained. It becomes possible to produce a useful substance in Saccharomycopsis lipolytica as a host using the plasmid.
moter/terminator vector in a single step allows for conversion of the shuttle vector into a lytic vector of an exogenous gene. The presence of restriction endonuclease enzyme recognition sites facilitative of insertion of a viral functional late gene into the late gene promoter/terminator vector in a single step allows for conversion of the shuttle vector into a lytic vector.
5118668 VARIANTS OF BOVINE PANCREATIC TRYPSIN INHIBITOR AND PHARMACEUTICAL USE THEREOF Ernst-August Auerswald, Wolfgang Bruns, Dietrich Horlein, Gerd Reinhardt, Eugen Schnabel, Werner Schroder, Munich, Federal Republic Of Germany assigned to Bayer Aktiengesellschaft Peptides having essentially the sequence of bovine pancreatic trypsin inhibitor (aprotinin) wherein one or more of the amino acids at positions 15, 16, 17, 18, 34, 39 and 52 are replaced by any naturally occurring amino acid produced by recombinant DNA technology, process, expression vector and recominant host therefor and pharmaceutical use thereof. Such peptides being useful as therapeutic agents in diseases connected with the presence of excessive amounts of proteinases.
5118627 PAPOVA VIRUS CONSTRUCTION Jeffrey K Browne assigned to Amgen A microbial shuttle vector is disclosed which is independently replicative in bacterial cells and mammalian cells and includes in its DNA sequence bacterial plasmid sequences allowing selection and replication in bacterial cells, an SV40 viral origin of replication, and either an SV40 functional early gene promoter and functional early gene terminator or an SV40 functional late gene promoter and functional late gene terminator, the vector having a unique restriction endonuclease enzyme recognition site between the promoter and terminator for insertion of an exogenous gene. The presence of restriction endonuclease enzyme recognition sites facilitative of insertion of a viral functional late gene into the early gene pro-
5118792 ICE CRYSTAL GROWTH SUPPRESSION POLYPEPTIDES AND METHOD OF MAKING Gareth J Warren, Gunhild M Mueller, Robert McKown assigned to DNA Plant Technology Corporation Novel methods of improving freezing tolerance of organic materials through the use of antifreeze polypeptides is provided. These polypeptides increase the storage life of foodstuffs and biologics, as well as protect plant products, such as during growth. The antifreeze polypeptides, or their fusion proteins, may be produced chemically or by recombinant DNA techniques, and then purified for a variety of uses.