5157021 Insulin derivatives and pharmaceutical preparations containing these derivatives

169

PATENT ABSTRACTS

5156977

5157021

MONOCLONAL ANTIBODIES AGAINST ATRIAL. NATRIURETIC PEbl’IDES Oti HUMtiS, HYBRIDOMAS & METHODS OF USE

INSULIN DERIVATIVES AND PHARMACEUTICAL PREPARATIONS CONTAINING THESE DERIVATIVES

Caludi Hirth, Frank-Joachim Morich, Dieter Neuser, Johannes-Peter Stasch, Wuppertal, Federal Republic Of Germany assigned to Bayer Aktiengesellschaft Monoclonal antibodies against atrial, natriuretic peptides are produced by deposited hybridoma cell lines 85031401 and 85031402 deposited with the European Collection of Animal Cell Cultures. The hybridoma are produced by fusion with cells from mammals which produce antibodies against atria1 natriuretic peptides. The antibodies can thereafter be used in assays for the determination of the level of artrial natriuretic peptides in biological samples such as blood, plasma, serum, urine, lymph or cerebrospinal fluid.

Per Balschmidt, Finn B Hansen, Espergaerde, Denmark assigned to Novo Nordisk A/S Insulin derivatives in which from two to four amino acid residues of positions, A4, A17, B13 and B21 comprise an uncharged side chain and pharmaceutical preparations containing these insulin derivatives. The disclosed insulin derivatives and preparations possess protracted insulin activity. The protracted insulin activity is relatively insensitive to tryptic activity.

5157032 MIXED LIGAND COMPLEXES AND USES THEREOF AS BINDING AGENTS AND PROBES TO DNA Jacqueline K Barton assigned to The Trustees of Columbia University In The City of New York

5157020 SYNTHETIC

SENESCENT ANTIGEN

CELL

Marguerite M B Kay, John J Marchalonis signed to Research Corporation Tech Inc

as-

Disclosed herein is a synthetic senescent cell antigen comprised of purified peptides immunoreactive with antibodies to the naturally occurring antigen. Preferably, the synthetic senescent cell antigen comprises two peptides with the amino acid sequences SKLIKIFQDHPLQKTYN and LFKPPKYHPDVPYVKR, respectively. The antigen and peptides may be used in compositions, diagnostic kits, and methods for detecting or measuring antibodies to senescent cell antigen, studying cellular aging and autoimmune mechanisms, separating anions from a gas or liquid, or treating certain diseases.

This invention concerns a coordination complex or salt thereof which is spectroscopically or photoactively determinable when bound to DNA having the formula See Parent for Chemical Structure wherein M is a suitable transition metal and each of Rl, R2 and R3 is ethylenediamine, bipyridine, phenanthroline, diazafluorene-9-one or a substituted derivative thereof, or phenanthrenequinonediimine or a substituted derivative thereof, dypyridophenazine or a substituted derivativethereof; wherein Rl, R2 and R3 are bound to M by coordination bonds; provided that at least one of Rl, R2 or R3 is dypyridophenazine or a substituted derivative thereof. The invention also concerns a labeled DNA probe which comprises the complex covalently bound to the DNA probe. Further the invention concerns a method of detecting the presence in a sample a target DNA of interest which comprises contacting the sample containing the target DNA with a complementary labeled DNA probe under hybridizing conditions and measuring the resulting luminescense emitted from the labeled DNA probe, a change in the hnninescense as compared with the luminescense in the absence of the sample indicating the presence of the target DNA.