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5173403 Thermostable acid protease from sulfolobus acidocaldarius and gene
New Patents
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An oligonucleotid~ probe specific for Neisseria from the selected gonorrhoeae is oligonucleotides RS2* and RS3*, RSZ** and RS3**. It can have further nucleotides at its 5’ end and/or 3’ end. This probe is suitable for the specific detection of nucleic acids of the pathogenic species N. gonorrhoeae by hybrid formation under hybridization conditions.
identical to that of other aspartic proteases. Thermopsin hydrolyzes the following bonds: Leu-Val, Leu-Tyr, Phe-Phe, Phe-Tyr, and TyrThr, indicating that the specificity of thermopsin is similar to that of pepsin for the large hydrophobic residues at both sides of the scissile bond. In addition, thermospin is resistant to detergent inactivation, the protein retaining proteolytic activity even in the presence of high concentrations of sodium dodecyl sulfate.
5173402 METHOD AND COMPOSITIONS FOR SCREENING AND DIAGNOSING HUMAN CYTOMEGALOVTRUS ( HCMV ) Deborah H Spector, Stephen A Spector assigned to The Regents of the University of California DNA sequences of hCMV are obtained by restriction of the hCMV genome, the resulting fragments free of fragments cross-hybridizing with human DNA and other viruses may be used for hybridization with clinical samples suspected of containing hCMV to provide a sensitive method for detection of hCMV at low particle number.
5173403 THERMOSTABLE ACID PROTEASE FROM SULFOLOBUS ACIDOCALDARIUS AND GENE Jordan J N Tang, Xin-L Lin assigned to Oklahoma Medical Research Foundation A thermostable, very acidic protease, which has been named thermopsin, was purified to homogeneity from the culture medium of Sulfolobus acidocaldarius by a five-step procedure including column chromatographies on DEAESepharose CL-6B, phenyi-Sepharose CL4B, Sephadex G-100, MonoQ (FPLC), and gel iiltration (HPLC). The enzyme is a single polypeptide chain having proteolytic activity over pH range 0 to 11 at temperatures between 0 degrees and 100 degrees C., with maximal activity at approximately pH 2 and 90 degrees C. Antibodies directed against thermopsin have been prepared. Through studies using various aspartic protease inhibitors, thiol and metalloprotease inhibitors, and serine protease inhibitors, it was determined that, although similar to some aspartic proteases, the active site of thermopsin is clearly not
5173408 MAMMALIAN PANCREATIC CHOLESTEROL ESTERASE Louis G Lange, Curtis A Spilburg The invention provides methods for the purification to homogeneity of pancreatic cholesterol esterase in useful quanities from a variety of mammalian species. The gene for a mammalian pancreatic cholesterol esterase has been cloned and sequenced, and is useful for expressing cholesterol esterase in a transformed eukaryotic or prokaryotic cell culture. Thus, methods according to the invention enable the production of large quantities of pancreatic cholesterol esterase for the screening of inhibitors, the production of antibodies, and for commercial puralteration of related to the poses cholesterol/cholesterol ester composition of containing free or esterified materials cholesterol.
5173410 TRANSFER VECTOR Paul Ahlquist assigned to Lubrizol Genetics Inc A recombinant DNA vector is provided as a universal transcription vector having a replication origin and selectable marker, a promoter and a transcription initiation site comprising a first transcribed nucleotide, wherein a restriction site is provided immediately adjacent to and upstream from the transcription initiation site so as to seprate transcribed from untranscribed nucleotides. A second restriction site may also be positioned downstream from the said restriction site. Precise control of initiation and termination of transcription is attained by this invention. Such control is important in assuring the effectiveness of transcribed RNA viral vectors. A
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An oligonucleotid~ probe specific for Neisseria from the selected gonorrhoeae is oligonucleotides RS2* and RS3*, RSZ** and RS3**. It can have further nucleotides at its 5’ end and/or 3’ end. This probe is suitable for the specific detection of nucleic acids of the pathogenic species N. gonorrhoeae by hybrid formation under hybridization conditions.
identical to that of other aspartic proteases. Thermopsin hydrolyzes the following bonds: Leu-Val, Leu-Tyr, Phe-Phe, Phe-Tyr, and TyrThr, indicating that the specificity of thermopsin is similar to that of pepsin for the large hydrophobic residues at both sides of the scissile bond. In addition, thermospin is resistant to detergent inactivation, the protein retaining proteolytic activity even in the presence of high concentrations of sodium dodecyl sulfate.
5173402 METHOD AND COMPOSITIONS FOR SCREENING AND DIAGNOSING HUMAN CYTOMEGALOVTRUS ( HCMV ) Deborah H Spector, Stephen A Spector assigned to The Regents of the University of California DNA sequences of hCMV are obtained by restriction of the hCMV genome, the resulting fragments free of fragments cross-hybridizing with human DNA and other viruses may be used for hybridization with clinical samples suspected of containing hCMV to provide a sensitive method for detection of hCMV at low particle number.
5173403 THERMOSTABLE ACID PROTEASE FROM SULFOLOBUS ACIDOCALDARIUS AND GENE Jordan J N Tang, Xin-L Lin assigned to Oklahoma Medical Research Foundation A thermostable, very acidic protease, which has been named thermopsin, was purified to homogeneity from the culture medium of Sulfolobus acidocaldarius by a five-step procedure including column chromatographies on DEAESepharose CL-6B, phenyi-Sepharose CL4B, Sephadex G-100, MonoQ (FPLC), and gel iiltration (HPLC). The enzyme is a single polypeptide chain having proteolytic activity over pH range 0 to 11 at temperatures between 0 degrees and 100 degrees C., with maximal activity at approximately pH 2 and 90 degrees C. Antibodies directed against thermopsin have been prepared. Through studies using various aspartic protease inhibitors, thiol and metalloprotease inhibitors, and serine protease inhibitors, it was determined that, although similar to some aspartic proteases, the active site of thermopsin is clearly not
5173408 MAMMALIAN PANCREATIC CHOLESTEROL ESTERASE Louis G Lange, Curtis A Spilburg The invention provides methods for the purification to homogeneity of pancreatic cholesterol esterase in useful quanities from a variety of mammalian species. The gene for a mammalian pancreatic cholesterol esterase has been cloned and sequenced, and is useful for expressing cholesterol esterase in a transformed eukaryotic or prokaryotic cell culture. Thus, methods according to the invention enable the production of large quantities of pancreatic cholesterol esterase for the screening of inhibitors, the production of antibodies, and for commercial puralteration of related to the poses cholesterol/cholesterol ester composition of containing free or esterified materials cholesterol.
5173410 TRANSFER VECTOR Paul Ahlquist assigned to Lubrizol Genetics Inc A recombinant DNA vector is provided as a universal transcription vector having a replication origin and selectable marker, a promoter and a transcription initiation site comprising a first transcribed nucleotide, wherein a restriction site is provided immediately adjacent to and upstream from the transcription initiation site so as to seprate transcribed from untranscribed nucleotides. A second restriction site may also be positioned downstream from the said restriction site. Precise control of initiation and termination of transcription is attained by this invention. Such control is important in assuring the effectiveness of transcribed RNA viral vectors. A