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5192659 Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes
PATENT ABSTRACIS
5192508 CARBOHYDRATE CELLULAR ADHESION APPARATUS Ronald L Schnaar assigned to The Johns Hopkins University An apparatus is provided for determining binding of mammalian cells to carbohydrates on a chromatographic plate surface. The chromatogram is performed, the plate introduced into a cell adhesion chamber, and the chamber filled with an appropriate cell containing medium. After mild centrifugation to ensure cell carbohydrates interaction, nonspecific adherent cells are removed by gravitational force in the opposite direction. The plate is then carefully washed without subjecting the plate to any air/liquid interface, the cells fixed and the plate analyzed.
5192657 STABILIZED PROTEOLYTIC SOLUTION AND REAGENT KIT Janice Jakway, Dennis Mochnal assigned to Ortho Diagnostic Systems Inc A stabilized proteolytic solution has been developed which can be used to treat selected cells, especially red blood cells. A preferred stabilized solution of the invention comprises about 0.1% to about 0.3% ficin in a citratebuffered saline solution containing mannitol, Lcysteine, dithiothrietol in an active concentration of less than about 10 mM, and EDTA. A kit for qualitatively identifying unexpected blood group antibodies comprising a series of untreated and ficin-treated red blood cells, a stabilized ficin reagent, a red blood cell diluent and an enzyme control is also provided.
5192658 COMPOSITIONS AND PROTOCOLS APPLICABLE GENETIC ANALYSIS
TO
Zvi G Loewy, Nancy Y Ip, Howard J Baum, Susan L Leary, Stadt Ingrid L van de assigned to Lifecodes Corporation Polynucleotide sequences and other compositions useful for D N A polymorphism and other
159
genetic analyses are disclosed herein. Also disclosed is a method for obtaining Variable Tandem Repeat polymorphism at a single genetic locus as well as other genetic analyses.
5192659 INTRON SEQUENCE ANALYSIS METHOD FOR DETECTION OF ADJACENT AND REMOTE LOCUS ALLELES AS HAPLOTYPES Malcolm J Simons, Fryerstown, Australia assigned to GeneType AG The present invention provides a method for detection of at least one allele of a genetic locus and can be used to provide direct determination of the haplotype. The method comprises amplifying genomic D N A with a primer pair that spans an intron sequence and defines a D N A sequence in genetic linkage with an allele to be detected. The primer-defined D N A sequence contains a sufficient number of intron sequence nucleotides to characterize the allele. Genomic DNA is amplified to produce an amplified D N A sequence characteristic of the allele. The amplified D N A sequence is analyzed to detect the presence of a genetic variation in the amplified DNA sequence such as a change in the length of the sequence, gain or loss of a restriction site or substitution of a nucleotide. The variation is characteristic oftbe allele to be detected and can be used to detect remote alleles. Kits comprising one or more of the reagents used in the method are also described.
5192660 ELISA METHODS FOR THE DETERMINATION OF HUMAN PLATELET DERIVED GROWTH FACTOR (PDGF) DIMER FORMS PRESENT IN HUMAN TISSUES AND FLUIDS Berenice Reed-Gitomer assigned to The United States of America as represented by the Department of Health and Human Services Enzyme linked immunosorbent assay (ELISA) methods for quantitatively determining the level of PDGF-BB and PDGF-AB, or, PDGF-AA and PDGF-AB present in a human's bodily
5192508 CARBOHYDRATE CELLULAR ADHESION APPARATUS Ronald L Schnaar assigned to The Johns Hopkins University An apparatus is provided for determining binding of mammalian cells to carbohydrates on a chromatographic plate surface. The chromatogram is performed, the plate introduced into a cell adhesion chamber, and the chamber filled with an appropriate cell containing medium. After mild centrifugation to ensure cell carbohydrates interaction, nonspecific adherent cells are removed by gravitational force in the opposite direction. The plate is then carefully washed without subjecting the plate to any air/liquid interface, the cells fixed and the plate analyzed.
5192657 STABILIZED PROTEOLYTIC SOLUTION AND REAGENT KIT Janice Jakway, Dennis Mochnal assigned to Ortho Diagnostic Systems Inc A stabilized proteolytic solution has been developed which can be used to treat selected cells, especially red blood cells. A preferred stabilized solution of the invention comprises about 0.1% to about 0.3% ficin in a citratebuffered saline solution containing mannitol, Lcysteine, dithiothrietol in an active concentration of less than about 10 mM, and EDTA. A kit for qualitatively identifying unexpected blood group antibodies comprising a series of untreated and ficin-treated red blood cells, a stabilized ficin reagent, a red blood cell diluent and an enzyme control is also provided.
5192658 COMPOSITIONS AND PROTOCOLS APPLICABLE GENETIC ANALYSIS
TO
Zvi G Loewy, Nancy Y Ip, Howard J Baum, Susan L Leary, Stadt Ingrid L van de assigned to Lifecodes Corporation Polynucleotide sequences and other compositions useful for D N A polymorphism and other
159
genetic analyses are disclosed herein. Also disclosed is a method for obtaining Variable Tandem Repeat polymorphism at a single genetic locus as well as other genetic analyses.
5192659 INTRON SEQUENCE ANALYSIS METHOD FOR DETECTION OF ADJACENT AND REMOTE LOCUS ALLELES AS HAPLOTYPES Malcolm J Simons, Fryerstown, Australia assigned to GeneType AG The present invention provides a method for detection of at least one allele of a genetic locus and can be used to provide direct determination of the haplotype. The method comprises amplifying genomic D N A with a primer pair that spans an intron sequence and defines a D N A sequence in genetic linkage with an allele to be detected. The primer-defined D N A sequence contains a sufficient number of intron sequence nucleotides to characterize the allele. Genomic DNA is amplified to produce an amplified D N A sequence characteristic of the allele. The amplified D N A sequence is analyzed to detect the presence of a genetic variation in the amplified DNA sequence such as a change in the length of the sequence, gain or loss of a restriction site or substitution of a nucleotide. The variation is characteristic oftbe allele to be detected and can be used to detect remote alleles. Kits comprising one or more of the reagents used in the method are also described.
5192660 ELISA METHODS FOR THE DETERMINATION OF HUMAN PLATELET DERIVED GROWTH FACTOR (PDGF) DIMER FORMS PRESENT IN HUMAN TISSUES AND FLUIDS Berenice Reed-Gitomer assigned to The United States of America as represented by the Department of Health and Human Services Enzyme linked immunosorbent assay (ELISA) methods for quantitatively determining the level of PDGF-BB and PDGF-AB, or, PDGF-AA and PDGF-AB present in a human's bodily