- Email: [email protected]
5196524 Fusion reporter gene for bacterial luciferase
PATENT ABSTRACTS polypeptide. This invention also relates to pharmaceutically acceptable compositions and methods characterized by at least one of these natural or recombinant inldbitors of platelct activation, alone or in combination with conventional anti-thrombin compounds. The compositions, combinations and methods of this invention are particularlyusefulin the treatment of thrombotic diseases.
51965~ CONTROL OF GENE EXPRESSION BY GLUCOSE, CALCIUM AND TEMPERATURE A m y S Lee assigned to The University of Southern California The regulatory sequence for a glucose regulated protein has been isolated. W h e n fused into suitable expression vectors within reading relationship of a desired polypcptide D N A sequence, the resulting hybrid gene can be induced to high levelsby glucose starvation,or caleium shock, or by denaturing reagents such as beta-mercaptocthanol, in a variety of host systems. Further, the hybrid gene can also be regulated by temperature when it is introduced into temperature-sensitivehost systems such as the temperature-sensitive mutant of Chinese hamster cells or other systems having a compatible mutation.
5196524 FUSION REPORTER GENE FOR BACTERIAL LUC1FERASE Gary D Gustafson, Thomas D Ingolia, Gretchen Kirchner, Jean L Roberts, Rehovot, assigned to Eli Lilly and Company Novel fusion reporter genes, fusion reporter proteins, and an improved reporter system for measuring the relative activity of a promoter sequence. A luxAB fusion gene of the present invention is particularly useful as a reporter gene and is derived from the fusion o f a luxA gene and a luxB geue from Vibrio harveyi. The gene products of the luxA and luxB genes are the alphaand beta-subunits, respectively, of a bacterial luciferase. A fusion protein encoded by a luxAB fusion gene is a single active protein and is particularly useful as a reporter protein having luciferase activity. An advantage of such a repor-
123
ter system to assay gene expression in many cells which contain F M N H 2 , such as bacterial and yeast cells,isthat an immediate and quantitative assessment of gene expression may be made from real-time lightmeasurements using intact cells.
5196525 DNA CONSTRUCT FOR ENHANCING THE EFFICIENCY OF TRANSCRIPTION Joan C McPherson, Robert Kay, Vancouver, Canada assigned to University of British Columbia Novel transcription initiation regions that provide for enhanced transcription of a D N A sequence, particularly a plant sequence, are provided.
51965~ CDNA CLONE FOR T-CELL SUPPRESSOR INDUCER FACTOR Kenneth D Beaman assigned to University of Health Sciences/The Chicago Medical School The invention generally discloses the cloning and characterization of a nucleotide sequence encode for at least a part of primate T-cell suppressor inducer factor (TsFI) protein and in particular, a putative gene for TsFI produced by the murine cell line A. 1.1. The nucleotide sequence is 2936 bp in length comprising an encoding sequence 2565 bp in length and encoding a protein of 690 amino acids.
5198337 ASSAY FOR GENE DELETION OF GST-1 IN HUMAN SAMPLES BASED ON THE POLYMERASE CHAIN REACTION William D Henuer, Keniue E Comstock, Barbara J S Sanderson, Virginia J Claflin, Vancouver, assigned to State of Oregon Methods for the detection of GST-1 gene deletion in human blood or tissue samples. The poly-
51965~ CONTROL OF GENE EXPRESSION BY GLUCOSE, CALCIUM AND TEMPERATURE A m y S Lee assigned to The University of Southern California The regulatory sequence for a glucose regulated protein has been isolated. W h e n fused into suitable expression vectors within reading relationship of a desired polypcptide D N A sequence, the resulting hybrid gene can be induced to high levelsby glucose starvation,or caleium shock, or by denaturing reagents such as beta-mercaptocthanol, in a variety of host systems. Further, the hybrid gene can also be regulated by temperature when it is introduced into temperature-sensitivehost systems such as the temperature-sensitive mutant of Chinese hamster cells or other systems having a compatible mutation.
5196524 FUSION REPORTER GENE FOR BACTERIAL LUC1FERASE Gary D Gustafson, Thomas D Ingolia, Gretchen Kirchner, Jean L Roberts, Rehovot, assigned to Eli Lilly and Company Novel fusion reporter genes, fusion reporter proteins, and an improved reporter system for measuring the relative activity of a promoter sequence. A luxAB fusion gene of the present invention is particularly useful as a reporter gene and is derived from the fusion o f a luxA gene and a luxB geue from Vibrio harveyi. The gene products of the luxA and luxB genes are the alphaand beta-subunits, respectively, of a bacterial luciferase. A fusion protein encoded by a luxAB fusion gene is a single active protein and is particularly useful as a reporter protein having luciferase activity. An advantage of such a repor-
123
ter system to assay gene expression in many cells which contain F M N H 2 , such as bacterial and yeast cells,isthat an immediate and quantitative assessment of gene expression may be made from real-time lightmeasurements using intact cells.
5196525 DNA CONSTRUCT FOR ENHANCING THE EFFICIENCY OF TRANSCRIPTION Joan C McPherson, Robert Kay, Vancouver, Canada assigned to University of British Columbia Novel transcription initiation regions that provide for enhanced transcription of a D N A sequence, particularly a plant sequence, are provided.
51965~ CDNA CLONE FOR T-CELL SUPPRESSOR INDUCER FACTOR Kenneth D Beaman assigned to University of Health Sciences/The Chicago Medical School The invention generally discloses the cloning and characterization of a nucleotide sequence encode for at least a part of primate T-cell suppressor inducer factor (TsFI) protein and in particular, a putative gene for TsFI produced by the murine cell line A. 1.1. The nucleotide sequence is 2936 bp in length comprising an encoding sequence 2565 bp in length and encoding a protein of 690 amino acids.
5198337 ASSAY FOR GENE DELETION OF GST-1 IN HUMAN SAMPLES BASED ON THE POLYMERASE CHAIN REACTION William D Henuer, Keniue E Comstock, Barbara J S Sanderson, Virginia J Claflin, Vancouver, assigned to State of Oregon Methods for the detection of GST-1 gene deletion in human blood or tissue samples. The poly-