5352605 Chimeric genes for transforming plant cells using viral promoters

PATENT ABSTRACTS protease usually produced by Bacillus species by altering these DNA sequences in defined positions by directed mutagenesis (point mutation) in such a way that the codon in which the point mutation is located now codes for an amino acid which is more strongly basic than the original amino acid. The result is highly alkaline proteases in which original amino acids have been replaced by more strongly basic amino acids, preferably by the amino acids lysine or arginine. Synthetic oligonucleotides, DNA sequences, vectors and transformed microorganisms which are used for generating and obtaining the optimized highly alkaline protease are also described.

5352604 ALKALINE PROTEOLYTIC ENZYME AND METHOD OF PRODUCTION Wilson Charles R; Ladin Beth; Mielenz Jonathan; Hom Sherman S M; Hansen Dieter; Reynolds Robert; Kennedy Nicholas C T; Schindler Joachi; Bahn Michael; Schmid Rolf; Markgraf Martina; Paech Christian; Maurer Karlheinz Santa Rosa, CA, UNITED STATES Assigned to Henkel Research Corporation A proteolytic enzyme for use in detergent formulations having increased pH and oxidative stability under typical laundering conditions in aqueous solutions is produced by fermenting Bacillus ilcheniformis host strain transformed by a multicopy plasmid comprised of DNA sequences which code for the desired proteolytic enzyme.

5352605 CHIMERIC GENES FOR TRANSFORMING PLANT CELLS USING VIRAL PROMOTERS Fraley Robert T; Horsch Robert B; Rogers Stephen Ballwin, MO, UNITED STATES Assigned to Monsanto Company In one aspect the present invention relates to the use of viral promoters in the expression of chimeric genes in plant cells. In another aspect this invention relates to chimeric genes which are capable of being expressed in plant cells, which utilize promoter regions derived from viruses which are capable of infecting plant cells. One such virus comprises the cauliflower mosaic virus

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(CaM'V). Two differem promoter regions have been derived from the CaMV $enome and ligated to heteroiogo~s coding sequences to form chimeric genes. These chimeric genes have been shown to be expressed in plant ceils. This invention also relates to plant cells, plant tissue, and differentiated plants which contain and express the chimeric genes of this invention.

5352607 MOLECULAR CLONE OF A CHITINASE GENE FROM VIBRIO PARAHEMOLYTICUS Laine Roger A; Ou Chin-Yih; Jaynes Jesse M Baton Rouge, LA, UNITED STATES Assigned to Louisiana State University and Agricultural College A process for cloning the chitinnse gene of Vibrio parahemolyticus is provided, comprising the steps of cleaving the Vibrio parahemolytioas DNA with San3A, PSTI or other restriction enzyme, mixing the cleaved DNA fragments in the presence of pUCI8 and "1"4 ligase to produce a composite plasmid, and inserting the composite plasmid in a DH5a strain of E. Coil.

5352661 BACILLUS THURINGIENSIS ISOLATE DENOTED B.T. PS81GG, ACTIVE AGAINST LEPIDOPTERAN PESTS, AND A GENE ENCODING A LEPIDOPTERAN-ACTIVE TOXIN Payne Jewel; Sick August; Thompson Mar San Diego, CA, UNITED STATES Assigned to Mycogen Corporation A novel B.t. isolate with activity against lepidopteran insects is disclosed. This isolate is highly active against the beet armyworn~ A gene from this isolate has been cloned. The DNA encoding the B.t. toxin can be used to transform various prokaryotic and eukaryotic microbes to express the B.t. toxin. These recombinant microbes can be used to control lepidopteran insects in various environments.