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5374523 Allelic variants of bovine somatotropin ge
761
PATENT ABSTRACTS
5374518 MONOCLONAL ANTIBODY FOR DIFFERENTIATING HIV-2 FROM HIM-1 SEROPOS1TIVE INDIVIDUALS Hunt Jeffrey C; Satin Virender; Devare Sushii; Tribby Ilse I; Desai Suresh M; Casey James M Lindenhurst, IL, UNITED STATES Assigned to Abbott Laboratories A mouse monoclonal antibody is provided which detects HIV-2 seropositive individuals and differentiates them from HIV-1 seropositive individuals. The monoclonal antibody is specific for an epitope of HIV-2 gp41 which lies outside the characterized immunodominant region. The epitope recognized by the moncolonal antibody has the amino acid sequence HTTVPW.
5374519 OLIGOPEPTIDES COMPRISING PI8 PROTEIN OF HUMAN IMMUNODEFICIENCY VIRUS (HIV), COMPOSITIONS COMPRISING PEPTIDES OF P18 PROTEIN OF HIV, AND DIAGNOSTIC KITS AND METHODS FOR DETECTING ACQUIRED IMMUNE DEFICIENCY SYNDROME (AIDS) Montngnier Luc; Chermann Jean-Claude; BarreSinoussi Francoise; Vezinet-Bmn Francoise; Rouzioux Christine; Rozenbaum Willy; Dauguet Charles; Gruest Jacqueline; Nugeyre MarieTheresa; Rey Francoise; Axler-Bfin Claudine; Chnmaret Solange Le Plessis Robi, FRANCE Assigned to lnstitut Pasteur
5374520 ASSAY METHOD FOR EPSTEINBARR VIRUS ANTIBODIES Milman Gregory Baltimore, MD, UNITED STATES Assigned to The Johns Hopkins University Assay methods and kits for detecting and quantifying anti-Epstein-Barr Virus ( EBV ) antibodies in a sample. The methods comprise contacting samples with a free or immobilized synthetic antigenic E B V polypeptide that is recognized by human antibodies and detecting or quantifying immunocomplex formation. The polypeptides described are the carboxy-terminated one-third of the EBV Nuclear Antigen encoded by the EBV Barn H1 K-fragment (plasmid pNAK28), a 46-55 kDa EBV Early Antigen encoded by the 4340 base pair Bci 1 to Barn H1 restriction fragment within the EBV Barn H1 M-fragment (plasmid pEAl)), and an EBV viral capsid antigen. Bacterially synthesized fusion proteins ate recognized by human anti-EBV antibodies. The assay method is diagnostic for infectious mononucleosis, du'onic mononucleosis, Burkett's lymphoma and monopharyngeal carcinoma.
5374522 METHOD FOR RELEASING RNA AND DNA FROM CELLS Murlly Kathleen A; Epstein Barry; Rosen Ira; Dean Elizabeth D Spring Valley, CA, UNITED STATES Assigned to Gen-Probe Incorporated A method is disclosed for disrupting cells, including microorganisms, and facilitating thereby the release of cellular components including RNA and DNA into solution. Solutions or suspensions of cells are placed in a container with minute beads of various composition. The container is then placed in an ultrasound bath or otherwise subjected to sonieation until the cells disrupt releasing their cellular components, including RNA and DNA. The released RNA and DNA ate then available for hybridization with genetic probes.
5374523 Protein compositions containing the p18 and p25 proteins of the lymphadenopathy virus are used for detecting antibodies in blood serum as indicative of infection by such virus. The proteins can be used in various conventional ways to perform immunoassays for the detection of the antibodies.
ALLELIC VARIANTS OF BOVINE SOMATOTROPIN GE Collier Robert J; Hanser Scott; Krivi Gwen G; Lucy Matthew University City, MO, UNITED STATES Assigned to Monsanto Company
762
PATENT ABSTRACTS
An assay for determining the presence in bovine genetic material of a genetic marker located on the bovine somatotropin gene indicative of an inheritable trait of increased milk production is provided. The marker is a polymorphism in the gene encoding somatotropin at amino acid position 126 which results in two forms of somatotropin existing in bovine. The assay comprises analyzing a bovine to determine its genotype with respect to the somatotropin gene. Bovine with the desired marker can be selected for inclusion in breeding programs or for milking. The desired marker indicative of superior milk production is dependent upon the breed of cattle. Holstein cattle that are homozygous for the leucine forms of somatotropin are desired. Jersey cattle that are homozygous for the valine form of somatotropin are desired. A kit for performing the assay is also provided.
5374524 SOLUTION SANDWICH HYBRIDIZATION, CAPTURE AND DETECTION OF AMPLIFIED NUCLEIC ACIDS Miller Jeffrey A DenT, NH, UNITED STATES Assigned to E I Du Pont de Nemours and Company A nucleic acid probe assay which combines nucleic acid amplification with solution hybridization using capture and reporter pcobes followed by or simultaneously with immobilization on a solid suppcrt is provided. An assay sensitivity equal to 5 copies of HIV I DNA was achieved.
5374525 METHODS TO DETERMINE PREDISPOSITION TO HYPERTENSION AND ASSOCIATION OF VARIANT ANGIOTENSINOGEN GENIE AND HYPERTENSION Lalouel Jean-Marc; Jeunemaitre Xavier; Lifton Richard P; Soubrier Florant; Kotelevtsev Youri; Corral Pierre Salt Lake City, UT, UNITED STATES Assigned to University of Utah Research Foundation; Inse The association of molecular variants of the angiotensinogen gene with human hypertension is
disclosed. The determination of this association enables the screening of persons to identify those who have a predisposition to high blood presure.
5374526 METHOD FOR DETERMINING GENETIC MARKER FOR INCREASED PIG LITTER SIZE Rothschild Max E; Jacobson Carol D Ames, IA, UNITED STATES Assigned to Iowa State University Research Foundation Inc Disclosed herein are genetic markers for pig litter size, methods for identifying such markers, and methods of screening pigs to determine those more likely to produce larger litters. The markers are based upon the presence or absence of certain polymorphisms in the pig estrogen receptor gene. Preferably, the polymorphism is a restriction fragment length polymorphism (Rb'LP). A 3.7 kilobase fragment and a 4.3 kilobase fragment obtained by digesting pig genomic DNA with the restriction endonuclease Pvu II and detecting the fragments with a probe comprising a detectably labeled human estrogen receptor gene are associated with increased litter size.
~74~7 HIGH RESOLUTION DNA SEQUENCING METHOD USING LOW VISCOSITY MEDIUM Grossman Paul D Burfingame, CA, UNrl'ED STATES Assigned to Applied Biosystems Inc A DNA sequencing method for use in sequencing a DNA target sequence up to 300 bases, preferably up to 500 bases or greater in length, by electrophoretically separating a mixture of singlestranded DNA sequencing fragments in a capillary tube. The method employs an aqueous denaturing solution comprising between about 4 and about 7 weight percent linear polyaerylamide molecules having an average molecular weight of between about 20 and about 100 kDa. The low-viscosity of the solution allows rapid loading and reloading of such solution into the capillary tube.
PATENT ABSTRACTS
5374518 MONOCLONAL ANTIBODY FOR DIFFERENTIATING HIV-2 FROM HIM-1 SEROPOS1TIVE INDIVIDUALS Hunt Jeffrey C; Satin Virender; Devare Sushii; Tribby Ilse I; Desai Suresh M; Casey James M Lindenhurst, IL, UNITED STATES Assigned to Abbott Laboratories A mouse monoclonal antibody is provided which detects HIV-2 seropositive individuals and differentiates them from HIV-1 seropositive individuals. The monoclonal antibody is specific for an epitope of HIV-2 gp41 which lies outside the characterized immunodominant region. The epitope recognized by the moncolonal antibody has the amino acid sequence HTTVPW.
5374519 OLIGOPEPTIDES COMPRISING PI8 PROTEIN OF HUMAN IMMUNODEFICIENCY VIRUS (HIV), COMPOSITIONS COMPRISING PEPTIDES OF P18 PROTEIN OF HIV, AND DIAGNOSTIC KITS AND METHODS FOR DETECTING ACQUIRED IMMUNE DEFICIENCY SYNDROME (AIDS) Montngnier Luc; Chermann Jean-Claude; BarreSinoussi Francoise; Vezinet-Bmn Francoise; Rouzioux Christine; Rozenbaum Willy; Dauguet Charles; Gruest Jacqueline; Nugeyre MarieTheresa; Rey Francoise; Axler-Bfin Claudine; Chnmaret Solange Le Plessis Robi, FRANCE Assigned to lnstitut Pasteur
5374520 ASSAY METHOD FOR EPSTEINBARR VIRUS ANTIBODIES Milman Gregory Baltimore, MD, UNITED STATES Assigned to The Johns Hopkins University Assay methods and kits for detecting and quantifying anti-Epstein-Barr Virus ( EBV ) antibodies in a sample. The methods comprise contacting samples with a free or immobilized synthetic antigenic E B V polypeptide that is recognized by human antibodies and detecting or quantifying immunocomplex formation. The polypeptides described are the carboxy-terminated one-third of the EBV Nuclear Antigen encoded by the EBV Barn H1 K-fragment (plasmid pNAK28), a 46-55 kDa EBV Early Antigen encoded by the 4340 base pair Bci 1 to Barn H1 restriction fragment within the EBV Barn H1 M-fragment (plasmid pEAl)), and an EBV viral capsid antigen. Bacterially synthesized fusion proteins ate recognized by human anti-EBV antibodies. The assay method is diagnostic for infectious mononucleosis, du'onic mononucleosis, Burkett's lymphoma and monopharyngeal carcinoma.
5374522 METHOD FOR RELEASING RNA AND DNA FROM CELLS Murlly Kathleen A; Epstein Barry; Rosen Ira; Dean Elizabeth D Spring Valley, CA, UNITED STATES Assigned to Gen-Probe Incorporated A method is disclosed for disrupting cells, including microorganisms, and facilitating thereby the release of cellular components including RNA and DNA into solution. Solutions or suspensions of cells are placed in a container with minute beads of various composition. The container is then placed in an ultrasound bath or otherwise subjected to sonieation until the cells disrupt releasing their cellular components, including RNA and DNA. The released RNA and DNA ate then available for hybridization with genetic probes.
5374523 Protein compositions containing the p18 and p25 proteins of the lymphadenopathy virus are used for detecting antibodies in blood serum as indicative of infection by such virus. The proteins can be used in various conventional ways to perform immunoassays for the detection of the antibodies.
ALLELIC VARIANTS OF BOVINE SOMATOTROPIN GE Collier Robert J; Hanser Scott; Krivi Gwen G; Lucy Matthew University City, MO, UNITED STATES Assigned to Monsanto Company
762
PATENT ABSTRACTS
An assay for determining the presence in bovine genetic material of a genetic marker located on the bovine somatotropin gene indicative of an inheritable trait of increased milk production is provided. The marker is a polymorphism in the gene encoding somatotropin at amino acid position 126 which results in two forms of somatotropin existing in bovine. The assay comprises analyzing a bovine to determine its genotype with respect to the somatotropin gene. Bovine with the desired marker can be selected for inclusion in breeding programs or for milking. The desired marker indicative of superior milk production is dependent upon the breed of cattle. Holstein cattle that are homozygous for the leucine forms of somatotropin are desired. Jersey cattle that are homozygous for the valine form of somatotropin are desired. A kit for performing the assay is also provided.
5374524 SOLUTION SANDWICH HYBRIDIZATION, CAPTURE AND DETECTION OF AMPLIFIED NUCLEIC ACIDS Miller Jeffrey A DenT, NH, UNITED STATES Assigned to E I Du Pont de Nemours and Company A nucleic acid probe assay which combines nucleic acid amplification with solution hybridization using capture and reporter pcobes followed by or simultaneously with immobilization on a solid suppcrt is provided. An assay sensitivity equal to 5 copies of HIV I DNA was achieved.
5374525 METHODS TO DETERMINE PREDISPOSITION TO HYPERTENSION AND ASSOCIATION OF VARIANT ANGIOTENSINOGEN GENIE AND HYPERTENSION Lalouel Jean-Marc; Jeunemaitre Xavier; Lifton Richard P; Soubrier Florant; Kotelevtsev Youri; Corral Pierre Salt Lake City, UT, UNITED STATES Assigned to University of Utah Research Foundation; Inse The association of molecular variants of the angiotensinogen gene with human hypertension is
disclosed. The determination of this association enables the screening of persons to identify those who have a predisposition to high blood presure.
5374526 METHOD FOR DETERMINING GENETIC MARKER FOR INCREASED PIG LITTER SIZE Rothschild Max E; Jacobson Carol D Ames, IA, UNITED STATES Assigned to Iowa State University Research Foundation Inc Disclosed herein are genetic markers for pig litter size, methods for identifying such markers, and methods of screening pigs to determine those more likely to produce larger litters. The markers are based upon the presence or absence of certain polymorphisms in the pig estrogen receptor gene. Preferably, the polymorphism is a restriction fragment length polymorphism (Rb'LP). A 3.7 kilobase fragment and a 4.3 kilobase fragment obtained by digesting pig genomic DNA with the restriction endonuclease Pvu II and detecting the fragments with a probe comprising a detectably labeled human estrogen receptor gene are associated with increased litter size.
~74~7 HIGH RESOLUTION DNA SEQUENCING METHOD USING LOW VISCOSITY MEDIUM Grossman Paul D Burfingame, CA, UNrl'ED STATES Assigned to Applied Biosystems Inc A DNA sequencing method for use in sequencing a DNA target sequence up to 300 bases, preferably up to 500 bases or greater in length, by electrophoretically separating a mixture of singlestranded DNA sequencing fragments in a capillary tube. The method employs an aqueous denaturing solution comprising between about 4 and about 7 weight percent linear polyaerylamide molecules having an average molecular weight of between about 20 and about 100 kDa. The low-viscosity of the solution allows rapid loading and reloading of such solution into the capillary tube.