5380652 Device and procedure for identifying pathogenic microorganisms

PATENT ABSTRACTS Filed Sep. 3, 1987 PCT PUb. No. WO88/01746 PCT PUb. Date Mar. 10, 1988.In an assay in which a ligand is labelled by conjugation to a dihydrophtbalazinedione (DPD), e.g. luminol or isoluminol, and the conjugated DPD is reacted with an oxidant, e.g. hydrogen peroxide, and an active heme group catalyst, e.g. microperoxidase, the light intensity is enhanced by certain sterically hindered amines defined as saturated bicyclic compounds having a nitrogen atom at one or both bridgehead positions or a piperidine ring compound having four C1-4 alkyl groups at the 2and 6- positions. 1,4-Diazabicyclo(2.2.2)-octane, known as DABCO is preferred.

5380651 METHOD OF DETERMINING ODORANT COMPOUNDS AND ANTAGONISTS OF ODORANTS USING A PRIMARY CULTURE OF OLFACTORY NEURONS Ronnett Gabriele V; Hester Lynda; Snyder Solomon Baltimore, MD, UNITED STATES Assigned to The Johns Hopkins University Primary cultures of purified olfactory neurons can be stimulated with physiological levels of odorants. The neurons of the cukures express markers characteristic of mature olfactory neurons in vivo, such as vimentin, olfactory marker protein and neuron-specific enolase. The cultures are useful for screening for odorants and antagonists, as well as for biochemical and physiological studies of olfactory transduction. The olfactory neurons may comprise at least about 85% of cells in the culture. The olfactory neurons demonstrate responsiveness in culture to IBMP, citraliva, and isovaleric acid.

5380652 DEVICE AND PROCEDURE FOR IDENTIFYING PATHOGENIC MICROORGANISMS Ayres William; Duda John 15421, UNITED STATES

Chalk Hill, PA,

Pathogenic microorganisms such as Staphylococcus anreus are differentiated and identified by observing the selective inhibition of the microorganism which occurs when it is contacted with Alphazurine A dye.

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5380656 CHYMOPAPAIN AND METHOD OF PURIFYING IT ON AN INHIBITORY DIPEPTIDE AFFINITY COLUMN Barrett Alan J; Buttle David J; Rich Daniel H Cambridgeshire, UNITED KINGDOM Assigned to The Boots Company PLC PCT No. PCT/EP90/00647 Sec. 371 Date Dec. 13, 1991 See. 102(e) Date Dec. 13, 1991 PCT Filed Apr. 27, 1990 PCT Pub. No. WO90/13561 PCT Pub. Date Nov. 15, 1990.A method of purifying chymopapaln by active site directed affinity chromatography using inhibitory dipeptides as affinity ligands is presented. The inhibitory dipeptidus have C-terminal aldehyde derivatives of phenylalanine selected from the group consisting of the semicarbazone, the methoxyimine and the oxime. The purified chymopapain has a specific activity against BAPNA (1 raM) at 40 degrees C. and pH 6.8 of between 3000 and 4500 units per nag and contains less than 0.2% each of papaya proteinase III (PPIII), papaln and papaya proteinase IV (PPIV). The use of purified chymopapain in pharmaceutical compositions for the treatment of damaged mammalian spinal discs is also disclosed.

5380662 HYBRIDIZATION INCUBATOR WITH ROTISSERIE MECHANISM Robbins Arthur; Robbins Michael Mountain View, CA, UNITED STATES Assigned to Robbins Scientific Corporation A hybridization incubator assembly (10) is provided for incubation of samples contained in elongated sample bottles (16) and short sample bottles (60). The incubator (10) includes and oven body (18) which encloses a rotisserie assembly (14) adapted for carrying and agitating the sample bottles. The rotisserie assembly (14) includes a pair of bottle support wheels (43) mounted eccentrically on a drive shaft (40). The first wheel (44) and the second wheel (46) are mounted at different degrees of eccentricity and are axially separated on the drive shaft (40) by a distance slightly less than the length of the elongated bottles (16). Each support wheel (43) includes a plurality of grasping damps (60) affixed about the