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5407798 Amplification of midivariant DNA templates
PATENT ABSTRACTS
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5407795 CMV PROBES FOR USE IN SOLUTION PHASE SANDWICH Kolberg Janice A; Shen Lu-Ping; Urdea Michael S Hercules, CA, UNITED STATES Assigned to Chiron Corporation Novel DNA probe sequences for detection of CMV in a sample in a solution phase sandwich hybridization assay are described. Amplified nucleic acid hybridization assays using the probes are exemplified.
5407796 CYSTIC FIBROSIS M U T A T I O N CLUSTER Cutting Garry R; Antonarakis Stylianos E; Kazazian Haig H Towson, MD, UNITED STATES Assigned to The Johns Hopkins University Four mutations have been found clustered in exon 11 of the CFTR (cystic fibrosis transmembrane conductance regulator) gene. These mutations occur within a set of amino acids highly conserved among ATP-dependent transport proteins. Humans can be tested to determine whether they carry one of these mutations using a number of methods and/or probes taught herein. Specifically the mutations include: Asn549, Asp551, Stop553, and Thr559.
5407798 A M P L I F I C A T I O N OF MIDIVARIANT DNA TEMPLATES Martinelli Richard A; Donahue Jeffrey J; Unger John T Brighton, MA, UNITED STATES Assigned to Ciba Coming Diagnostics Corp
target specific nucleic acid sequence (probe) are described. An amplification method including the steps of hybridization and ligation of the midivariant DNA/probe conjugates followed by replication of the DNA template has enabled detection of less than 200 template molecules. In a modified amplification method one of the midivariant DNA/prohe conjugates further includes a RNA polymerase promoter sequence to enable transcription of the midivariant DNA template into RNA. The sequential ligationtranscription-replication method enables the detection of a single template molecule. The detectable molecule(s) are indicative of the presence of the target nucleic acid sequences in the test sample.
5407799 METHOD FOR HIGH-VOLUME S E Q U E N C I N G OF N U C L E I C ACI Studier F William Stony Brook, NY, UNITED STATES Assigned to Associated Universities Inc Random and directed priming methods for determining nucleotide sequences by enzymatic sequencing techniques, using libraries of primers of lengths 8, 9 or I0 bases, are disclosed. These methods permit direct sequencing of nucleic acids as large as 45,000 base pairs or larger without the necessity for subcloning. Individual primers are used repeatedly to prime sequence reactions in many different nucleic acid molecules. Libraries containing as few as 10,000 octamers, 14,200 nonamers, or 44,000 decamers would have the capacity to determine the sequence of almost any cosmid DNA. Random priming with a fixed set of primers from a smaller library can also be used to initiate the sequencing of individual nucleic acid molecules, with the sequence being completed by directed priming with primers from the library. In contrast to random cloning techniques, a combined random and directed priming strategy is far more efficient.
5407800 New methods are provided for the amplification of a midivariant DNA containing an inserted target specific nucleic acid sequence(s) to enable detection of the presence of a target nucleic acid sequence in a test sample. One method employs midivariant DNA as a template for the synthesis of RNA catalyzed by QB replicase. Two midivariant DNA/probe conjugates including a nonreplicable portion of midivariant DNA and a
REVERSE TRANSCRIPTION WITH THERMUS THERMOPHILUS POLYMERASE Gelfand David H; Myers Thomas W Oakland, CA, UNITED STATES Assigned to Hoffmann-La Roche lnc
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5407795 CMV PROBES FOR USE IN SOLUTION PHASE SANDWICH Kolberg Janice A; Shen Lu-Ping; Urdea Michael S Hercules, CA, UNITED STATES Assigned to Chiron Corporation Novel DNA probe sequences for detection of CMV in a sample in a solution phase sandwich hybridization assay are described. Amplified nucleic acid hybridization assays using the probes are exemplified.
5407796 CYSTIC FIBROSIS M U T A T I O N CLUSTER Cutting Garry R; Antonarakis Stylianos E; Kazazian Haig H Towson, MD, UNITED STATES Assigned to The Johns Hopkins University Four mutations have been found clustered in exon 11 of the CFTR (cystic fibrosis transmembrane conductance regulator) gene. These mutations occur within a set of amino acids highly conserved among ATP-dependent transport proteins. Humans can be tested to determine whether they carry one of these mutations using a number of methods and/or probes taught herein. Specifically the mutations include: Asn549, Asp551, Stop553, and Thr559.
5407798 A M P L I F I C A T I O N OF MIDIVARIANT DNA TEMPLATES Martinelli Richard A; Donahue Jeffrey J; Unger John T Brighton, MA, UNITED STATES Assigned to Ciba Coming Diagnostics Corp
target specific nucleic acid sequence (probe) are described. An amplification method including the steps of hybridization and ligation of the midivariant DNA/probe conjugates followed by replication of the DNA template has enabled detection of less than 200 template molecules. In a modified amplification method one of the midivariant DNA/prohe conjugates further includes a RNA polymerase promoter sequence to enable transcription of the midivariant DNA template into RNA. The sequential ligationtranscription-replication method enables the detection of a single template molecule. The detectable molecule(s) are indicative of the presence of the target nucleic acid sequences in the test sample.
5407799 METHOD FOR HIGH-VOLUME S E Q U E N C I N G OF N U C L E I C ACI Studier F William Stony Brook, NY, UNITED STATES Assigned to Associated Universities Inc Random and directed priming methods for determining nucleotide sequences by enzymatic sequencing techniques, using libraries of primers of lengths 8, 9 or I0 bases, are disclosed. These methods permit direct sequencing of nucleic acids as large as 45,000 base pairs or larger without the necessity for subcloning. Individual primers are used repeatedly to prime sequence reactions in many different nucleic acid molecules. Libraries containing as few as 10,000 octamers, 14,200 nonamers, or 44,000 decamers would have the capacity to determine the sequence of almost any cosmid DNA. Random priming with a fixed set of primers from a smaller library can also be used to initiate the sequencing of individual nucleic acid molecules, with the sequence being completed by directed priming with primers from the library. In contrast to random cloning techniques, a combined random and directed priming strategy is far more efficient.
5407800 New methods are provided for the amplification of a midivariant DNA containing an inserted target specific nucleic acid sequence(s) to enable detection of the presence of a target nucleic acid sequence in a test sample. One method employs midivariant DNA as a template for the synthesis of RNA catalyzed by QB replicase. Two midivariant DNA/probe conjugates including a nonreplicable portion of midivariant DNA and a
REVERSE TRANSCRIPTION WITH THERMUS THERMOPHILUS POLYMERASE Gelfand David H; Myers Thomas W Oakland, CA, UNITED STATES Assigned to Hoffmann-La Roche lnc