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5460974 Method of assaying whole blood for HDL cholesterol
PATENT ABSTRACTS
5460949 METHOD AND COMPOSITION FOR INCREASING THE ACCUMULATION OF SQUALENE AND SPECIFIC STEROLS IN YEAST Saunders Court A; Wolf Fred R; Mukharji Indrani Clarendon Hills, IL, UNITED STATES assigned to Amoco Corporation A method of increasing the accumulation of squalene and specific sterols in yeast comprising increasing the expression level of a structural gene encoding a polypeptide having HMG-CoA reductase activity in a mutant yeast having single or double defects in the expression of sterol biosynthetic enzymes is provided. The expression level of a structural gene is preferably increased by transforming yeast with a recombinant DNA molecule comprising a vector operatively linked to an exogenous DNA segment that encodes a polypeptide having HMG-CoA reductase activity and a promoter that is suitable for driving the expression of the encoded polypeptide in the transformed yeast. The polypeptide having HMG-CoA reductase activity is preferably a truncated, active HMG-CoA reductase enzyme. Recombinant DNA molecules useful for transforming yeast and mutant yeast transformed with such recombinant DNA molecules are also disclosed.
5460969 METHOD FOR DIFFERENTIATING THE SOURCE OF OCCULT GASTROINTESTINAL BLEEDING Fielder Paul N; Levine Robert A; Wardlaw Stephen C New Haven, CT, UNITED STATES The presence of fecal occult blood in a stool sample is detected by mixing a liquid stool sample with an acidic liquid, such as a phosphate/citrate buffer, to precipitate hematin from the solution. The precipitated hematin is separated and the presence or absence of
551
hemoglobin is determined by exposing the solution to a peroxidase diagnostic assay. A positive response indicates the presence of blood originating in the lower gastrointestinal tract, a leading indicator of lower GI cancer.
5460970 SEPARATION OF ACETALDEHYDE-INDUCED HEMOGLOBIN (HB A1-ACH) Truitt Edward B; Hazelett Susan; Liebelt Robert Kent, OH, UNITED STATES assigned to Summa Health System A chromatographic method used to detect chronic alcohol usage and alcoholism and to monitor alcohol rehabilitation treatment; the method provides improved resolution of the Hb A1-AcH peak from the contiguous Hb Ale peak by means of a polyaspartic acid chromatographic column and a nonlinear buffer gradient; the resulting Hb AI-AcH peak is then evaluated to determine chronic alcohol usage which is indicative of alcoholism and which can be used to monitor alcohol rehabilitation treatment.
5460974 METHOD OF ASSAYING WHOLE BLOOD FOR HDL CHOLESTEROL Kozak Mary B; Badke Andrea Osceola, IN, UNITED STATES assigned to Miles Inc An improved device and method of a) separating the cellular components of whole blood from plasma or serum, b) separating the low density lipoprotein (LDL) fraction and the very low density lipoprotein (VLDL) fraction from the plasma or serum, then c) assaying the plasma or serum for cholesterol presont in the high density lipoprotein (HDL) fraction are disclosed. The device includes a separation area that separates the cellular components of whole blood from the serum or plasma and separates the LDL and VLDL fractions from the serum or plasma, and a test area that assays the serum or plasma for the concentration of the HDL cholesterol. The
552
PATENT ABSTRACTS
method includes introducing a whole blood sample to a test device including a separation area comprising a first zone that separates the cellular components of the whole blood from the plasma or serum; and a second zone that separates the LDL and the VLDL fractions from the plasma or serum. The essentially cell-free, LDL-free and VLDL-free plasma or serum then migrates to a test area. ARer the plasma or serum migrates to the test area, plasma or serum is assayed for HDL cholesterol and the test area is examined for a quantitative response to HDL cholesterol present in the whole blood sample.
5460979
5461143 OLIGOSACCHARIDE ENZYME SUBSTRATES AND INHIBITORS Wong Chi-Huey; lchikawa Yoshitaka; Shen Gwo-Jenn San Diego, CA, UNITED STATES assigned to The Scripps Research Institute Oligosaccharide compounds that are substrates and inhibitors of glycosyltransferase and glycosidase enzymes and compositions containing such compounds are disclosed. A method of glycosylation is also disclosed. An E. coli transformed with phagemid CMPSIL-I, which phagemid comprises a gene for a modified CMP-sialic acid synthetase enzyme, which transformed E. coli has the ATCC accession No. 68531 is also provided.
INDIRECT FLUORESCENT ASSAY OF BLOOD SAMPLES Levine Robert A; Wardlaw Stephen C; Terstappen Leon W M M; Mercolino Thomas J; Recktenwald Diether J Guilford, CT, UNITED STATES assigned to Becton Dickinson and Company A patient's health is diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more groups of particles such as lyposomes or plastic beads of different densities for each group. Each group of density-defined particles carries antigens or antibodies which are specific to a complement antigen or antibody which may be in the blood sample being tested, and which are indicative of the patient's health. A label-tagged antibody which is specific to all bound antibody/antigen couples is added to the blood sample so as to form labelled antibody+antigen-antibody complexes (AAAC) in the blood sample. Upon centrifugation, the complexed particles will settle out in different areas in the tube according to the respective density of the particles, and the degree of label emission of the particle layers can enable qualitative or quantitative analyses of the blood sample to be made. Unbound labelled antibodies will be washed away from the complexed layers by the washing action of the descending blood ceils during the centrifugation step. Unbound labelled antibodies will thus not interfere with the analysis.
5461145 SEXING METHOD OF BOVINE EMBYROS Kudo Toshiyuki; Itagaki Yoshiaki; Sato Seiji; Sutou Shizuyo; Nakamura Toyoo Ibaraki, JAPAN assigned to Itoham Foods Inc The present invention provides PCR primers with which sexing of bovine embryos can be easily attained and provides a practical, rapid and reliable method for determining the sex of bovine embryos using these primers. The methods for determining the sex of the bovine embryos are characterized by discriminating PCR products which are obtained by amplifying specific DNA sequences by PCR with pairs of male-specific and gender-neutral primers. These primers are derived from DNAs which specifically hybridize to the bovine male genome and from DNAs which gender-neutrally hybridize to both bovine male and female genomes.
5460949 METHOD AND COMPOSITION FOR INCREASING THE ACCUMULATION OF SQUALENE AND SPECIFIC STEROLS IN YEAST Saunders Court A; Wolf Fred R; Mukharji Indrani Clarendon Hills, IL, UNITED STATES assigned to Amoco Corporation A method of increasing the accumulation of squalene and specific sterols in yeast comprising increasing the expression level of a structural gene encoding a polypeptide having HMG-CoA reductase activity in a mutant yeast having single or double defects in the expression of sterol biosynthetic enzymes is provided. The expression level of a structural gene is preferably increased by transforming yeast with a recombinant DNA molecule comprising a vector operatively linked to an exogenous DNA segment that encodes a polypeptide having HMG-CoA reductase activity and a promoter that is suitable for driving the expression of the encoded polypeptide in the transformed yeast. The polypeptide having HMG-CoA reductase activity is preferably a truncated, active HMG-CoA reductase enzyme. Recombinant DNA molecules useful for transforming yeast and mutant yeast transformed with such recombinant DNA molecules are also disclosed.
5460969 METHOD FOR DIFFERENTIATING THE SOURCE OF OCCULT GASTROINTESTINAL BLEEDING Fielder Paul N; Levine Robert A; Wardlaw Stephen C New Haven, CT, UNITED STATES The presence of fecal occult blood in a stool sample is detected by mixing a liquid stool sample with an acidic liquid, such as a phosphate/citrate buffer, to precipitate hematin from the solution. The precipitated hematin is separated and the presence or absence of
551
hemoglobin is determined by exposing the solution to a peroxidase diagnostic assay. A positive response indicates the presence of blood originating in the lower gastrointestinal tract, a leading indicator of lower GI cancer.
5460970 SEPARATION OF ACETALDEHYDE-INDUCED HEMOGLOBIN (HB A1-ACH) Truitt Edward B; Hazelett Susan; Liebelt Robert Kent, OH, UNITED STATES assigned to Summa Health System A chromatographic method used to detect chronic alcohol usage and alcoholism and to monitor alcohol rehabilitation treatment; the method provides improved resolution of the Hb A1-AcH peak from the contiguous Hb Ale peak by means of a polyaspartic acid chromatographic column and a nonlinear buffer gradient; the resulting Hb AI-AcH peak is then evaluated to determine chronic alcohol usage which is indicative of alcoholism and which can be used to monitor alcohol rehabilitation treatment.
5460974 METHOD OF ASSAYING WHOLE BLOOD FOR HDL CHOLESTEROL Kozak Mary B; Badke Andrea Osceola, IN, UNITED STATES assigned to Miles Inc An improved device and method of a) separating the cellular components of whole blood from plasma or serum, b) separating the low density lipoprotein (LDL) fraction and the very low density lipoprotein (VLDL) fraction from the plasma or serum, then c) assaying the plasma or serum for cholesterol presont in the high density lipoprotein (HDL) fraction are disclosed. The device includes a separation area that separates the cellular components of whole blood from the serum or plasma and separates the LDL and VLDL fractions from the serum or plasma, and a test area that assays the serum or plasma for the concentration of the HDL cholesterol. The
552
PATENT ABSTRACTS
method includes introducing a whole blood sample to a test device including a separation area comprising a first zone that separates the cellular components of the whole blood from the plasma or serum; and a second zone that separates the LDL and the VLDL fractions from the plasma or serum. The essentially cell-free, LDL-free and VLDL-free plasma or serum then migrates to a test area. ARer the plasma or serum migrates to the test area, plasma or serum is assayed for HDL cholesterol and the test area is examined for a quantitative response to HDL cholesterol present in the whole blood sample.
5460979
5461143 OLIGOSACCHARIDE ENZYME SUBSTRATES AND INHIBITORS Wong Chi-Huey; lchikawa Yoshitaka; Shen Gwo-Jenn San Diego, CA, UNITED STATES assigned to The Scripps Research Institute Oligosaccharide compounds that are substrates and inhibitors of glycosyltransferase and glycosidase enzymes and compositions containing such compounds are disclosed. A method of glycosylation is also disclosed. An E. coli transformed with phagemid CMPSIL-I, which phagemid comprises a gene for a modified CMP-sialic acid synthetase enzyme, which transformed E. coli has the ATCC accession No. 68531 is also provided.
INDIRECT FLUORESCENT ASSAY OF BLOOD SAMPLES Levine Robert A; Wardlaw Stephen C; Terstappen Leon W M M; Mercolino Thomas J; Recktenwald Diether J Guilford, CT, UNITED STATES assigned to Becton Dickinson and Company A patient's health is diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more groups of particles such as lyposomes or plastic beads of different densities for each group. Each group of density-defined particles carries antigens or antibodies which are specific to a complement antigen or antibody which may be in the blood sample being tested, and which are indicative of the patient's health. A label-tagged antibody which is specific to all bound antibody/antigen couples is added to the blood sample so as to form labelled antibody+antigen-antibody complexes (AAAC) in the blood sample. Upon centrifugation, the complexed particles will settle out in different areas in the tube according to the respective density of the particles, and the degree of label emission of the particle layers can enable qualitative or quantitative analyses of the blood sample to be made. Unbound labelled antibodies will be washed away from the complexed layers by the washing action of the descending blood ceils during the centrifugation step. Unbound labelled antibodies will thus not interfere with the analysis.
5461145 SEXING METHOD OF BOVINE EMBYROS Kudo Toshiyuki; Itagaki Yoshiaki; Sato Seiji; Sutou Shizuyo; Nakamura Toyoo Ibaraki, JAPAN assigned to Itoham Foods Inc The present invention provides PCR primers with which sexing of bovine embryos can be easily attained and provides a practical, rapid and reliable method for determining the sex of bovine embryos using these primers. The methods for determining the sex of the bovine embryos are characterized by discriminating PCR products which are obtained by amplifying specific DNA sequences by PCR with pairs of male-specific and gender-neutral primers. These primers are derived from DNAs which specifically hybridize to the bovine male genome and from DNAs which gender-neutrally hybridize to both bovine male and female genomes.