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5466602 Apparatus for growing tissue cultures in vitro
PATENT ABSTRACTS
5466586 METHOD FOR THE SYNTHESIS OF RIBONUCLEIC ACID (RNA) Davey Cheryl; Malek Lawrence T; Lens Peter; Wielaard Frits Toronto, CANADA assigned to Akzo Nobel NV The invention relates to a method for the synthesis of ribonucleic acid (RNA) starting from deoxyribonucleic acid (DNA). In the method of the invention, a double stranded DNA molecule is cleaved with a restriction endonuclease so as to generate a free 3’-end. The resultant DNA molecule is then denatured and hybridized with a primer that anneals to the 3’-end of the denatured DNA. The primer used in the hybridization contains a T7 promoter sequence at its S-end. Following the hybridization step, the 3’-ends of the resultant hybrid DNA molecule are extended with DNA polymerase to generate a template suitable for T7 RNA polymerase mediated RNA synthesis.
5466602 APPARATUS
FOR GROWING
TISSUE CULTURES
IN VITRO
Lyman George; Mathus Gregory; Root David Cape Porpoise, ME, UNITED STATES assigned to Data Packaging Corporation The present invention relates to an apparatus for growing tissue culture in vitro. The apparatus features a housing which fits into a well containing media. A
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bottom membrane surface is maintained at a depth within the well, and the housing centered in the well by a flange and rim which cooperate with the well.
5466603 TEMPERATURE REGULATED HYBRIDIZATION CHAMBER Meehan Brian; Heine Uwe; Mason James East Lansing, MI, UNITED STATES A temperature regulated hybridization chamber which includes an outer container which defines a first chamber for receiving a first fluid having a predetermined temperature. The hybridization chamber includes an inner container having a generally horizontal bottom wall with a periphery and an inner container side wall extending generally upwardly from the periphery of the inner container bottom wall. The inner container bottom wall and the inner container side wall define a second chamber for receiving a second fluid. The inner container is positioned within the first chamber with the inner container bottom wall and the inner container side wall being spaced from the outer container bottom wall and the outer container side wall, respectively, such that when the first fluid is positioned within the first chamber the first fluid is in engagement with the inner container bottom wall and inner container side wall. A drain conduit is in fluid communication with the second chamber for removing the second fluid from the second chamber. The drain conduit is positioned such that the second fluid flows through the drain conduit due to the force of gravity such that the second fluid in the inner chamber is maintained at a temperature which is about equal to the predetermined temperature of the first fluid.
5466586 METHOD FOR THE SYNTHESIS OF RIBONUCLEIC ACID (RNA) Davey Cheryl; Malek Lawrence T; Lens Peter; Wielaard Frits Toronto, CANADA assigned to Akzo Nobel NV The invention relates to a method for the synthesis of ribonucleic acid (RNA) starting from deoxyribonucleic acid (DNA). In the method of the invention, a double stranded DNA molecule is cleaved with a restriction endonuclease so as to generate a free 3’-end. The resultant DNA molecule is then denatured and hybridized with a primer that anneals to the 3’-end of the denatured DNA. The primer used in the hybridization contains a T7 promoter sequence at its S-end. Following the hybridization step, the 3’-ends of the resultant hybrid DNA molecule are extended with DNA polymerase to generate a template suitable for T7 RNA polymerase mediated RNA synthesis.
5466602 APPARATUS
FOR GROWING
TISSUE CULTURES
IN VITRO
Lyman George; Mathus Gregory; Root David Cape Porpoise, ME, UNITED STATES assigned to Data Packaging Corporation The present invention relates to an apparatus for growing tissue culture in vitro. The apparatus features a housing which fits into a well containing media. A
113
bottom membrane surface is maintained at a depth within the well, and the housing centered in the well by a flange and rim which cooperate with the well.
5466603 TEMPERATURE REGULATED HYBRIDIZATION CHAMBER Meehan Brian; Heine Uwe; Mason James East Lansing, MI, UNITED STATES A temperature regulated hybridization chamber which includes an outer container which defines a first chamber for receiving a first fluid having a predetermined temperature. The hybridization chamber includes an inner container having a generally horizontal bottom wall with a periphery and an inner container side wall extending generally upwardly from the periphery of the inner container bottom wall. The inner container bottom wall and the inner container side wall define a second chamber for receiving a second fluid. The inner container is positioned within the first chamber with the inner container bottom wall and the inner container side wall being spaced from the outer container bottom wall and the outer container side wall, respectively, such that when the first fluid is positioned within the first chamber the first fluid is in engagement with the inner container bottom wall and inner container side wall. A drain conduit is in fluid communication with the second chamber for removing the second fluid from the second chamber. The drain conduit is positioned such that the second fluid flows through the drain conduit due to the force of gravity such that the second fluid in the inner chamber is maintained at a temperature which is about equal to the predetermined temperature of the first fluid.