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5550037 Mammalian augmenter of liver regeneration (ALR)
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PATENT ABSTRACTS
5548076 METHOD OF MAKING OLIGONUCLEOTIDES, OLIGONUCLEOSIDE THIOPHOSPHATES AND OLIGONUCLEOSIDE PHOSPHOTRIESTERS USING H-PHOSPHONATE INTERMEDIATES
expressing a foreign gene in the cytoplasm of a eukaryotic cell is also disclosed which comprises incorporating into the cytoplasm a DNA-based cytoplasmic virus, a suitable carrier comprising a gene for an RNA polymerase which gene is foreign to the carder and to the cells, and a suitable carrier comprising a functional, cistron including a foreign gene flanked by a promotor sequence which is recognized bythe RNA polymerase.
Froehler Brian C; Matleucci Mark D Belmont, CA, UNITED STATES Assigned to Genentech Inc A method is provided for the high fidelity, rapid and economical in vitro synthesis of oligonucleotides. Nucleoside H-phosphonates are condensed in seriatim using a dehydrating agent to produce a poly (nucleoside H-phosphonate). The product is oxidized to yield the desired oligonucteotide. A novel reagent is provided for multiple nucleoside additions in single cycles.
5550035 PROKARYOTIC EXPRESSION IN EUKARYOTIC CELLS Moss Bernard; Studier F Willia; Fuerst Thomas R; Niles Edward G Bethesda, MD, UNITED STATES Assigned to The Research Foundation of State University of New York
A transient expression system is disclosed that utilizes bacteriophage RNA polymerase in the presence of a DNA-based cytoplasmic virus to facilitate expression of a foreign gene in the cytoplasm of a eukaryotic cell. A method of
5550037 MAMMALIAN AUGMENTER OF LIVER REGENERATION (ALR) Francavilla Antonio; Hagiya Michio; Starzl Thomas Assigned to University of Pittsburgh; Toyobo Co Ltd Full-length cDNA clones have been isolated encoding purified augmenter of liver regeneration (ALR) polypeptides prepared from the cytosol of livers from human and from weanling rats. The 0.5 kb human ALR cDNA includes a 33 bp 5'-untranslated region, a 375 bp coding region and a 107 bp 3'-untranslated region. The cDNA encodes a protein consisting of 125 amino acids. The molecular weight of human ALR calculated from the cDNA was 15,028. A comparison of the sequences for the human ALR with those of the rat ALR shows 71% homology at the nucleotide level and 86% homology at the amino acid level. The most obvious immediate clinical use for the augmenter of liver regeneration is in the treatment of hepatic failure.
PATENT ABSTRACTS
5548076 METHOD OF MAKING OLIGONUCLEOTIDES, OLIGONUCLEOSIDE THIOPHOSPHATES AND OLIGONUCLEOSIDE PHOSPHOTRIESTERS USING H-PHOSPHONATE INTERMEDIATES
expressing a foreign gene in the cytoplasm of a eukaryotic cell is also disclosed which comprises incorporating into the cytoplasm a DNA-based cytoplasmic virus, a suitable carrier comprising a gene for an RNA polymerase which gene is foreign to the carder and to the cells, and a suitable carrier comprising a functional, cistron including a foreign gene flanked by a promotor sequence which is recognized bythe RNA polymerase.
Froehler Brian C; Matleucci Mark D Belmont, CA, UNITED STATES Assigned to Genentech Inc A method is provided for the high fidelity, rapid and economical in vitro synthesis of oligonucleotides. Nucleoside H-phosphonates are condensed in seriatim using a dehydrating agent to produce a poly (nucleoside H-phosphonate). The product is oxidized to yield the desired oligonucteotide. A novel reagent is provided for multiple nucleoside additions in single cycles.
5550035 PROKARYOTIC EXPRESSION IN EUKARYOTIC CELLS Moss Bernard; Studier F Willia; Fuerst Thomas R; Niles Edward G Bethesda, MD, UNITED STATES Assigned to The Research Foundation of State University of New York
A transient expression system is disclosed that utilizes bacteriophage RNA polymerase in the presence of a DNA-based cytoplasmic virus to facilitate expression of a foreign gene in the cytoplasm of a eukaryotic cell. A method of
5550037 MAMMALIAN AUGMENTER OF LIVER REGENERATION (ALR) Francavilla Antonio; Hagiya Michio; Starzl Thomas Assigned to University of Pittsburgh; Toyobo Co Ltd Full-length cDNA clones have been isolated encoding purified augmenter of liver regeneration (ALR) polypeptides prepared from the cytosol of livers from human and from weanling rats. The 0.5 kb human ALR cDNA includes a 33 bp 5'-untranslated region, a 375 bp coding region and a 107 bp 3'-untranslated region. The cDNA encodes a protein consisting of 125 amino acids. The molecular weight of human ALR calculated from the cDNA was 15,028. A comparison of the sequences for the human ALR with those of the rat ALR shows 71% homology at the nucleotide level and 86% homology at the amino acid level. The most obvious immediate clinical use for the augmenter of liver regeneration is in the treatment of hepatic failure.