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5583024 Recombinant expression of coleoptera luciferase
PATENT ABSTRACTS
this digestion. The antigenic material thus obtained is then combined with an appropriate adjuvant.
695
5583025 E N Z Y M E S F O R THE P R O D U C T I O N OF 2-KETO-L-GULONIC ACID Lazarus Robert A; Hurle Mark; Anderson Stephen; Powers David B Millbrae, CA, UNITED STATES Assigned to Rutgers The State University of New Jersey
5583024 R E C O M B I N A N T EXPRESSION OF C O L E O P T E R A L U C I F E R A S E McElroy Marlene D; McElroy W; Helinski Donald R; Wood Keith V; De Wet Jeffrey R; Ow David W; Howell Stephen H late of La Jolla, CA, UNITED STATES Assigned to The Regents of the University of California A method is disclosed for producing a protein which expresses bioluminescance activity which involves combining two polydeoxyribonucleotides, one containing a continuous sequence of codons encoding a polypeptide which comprises a single covalently bonded molecular structure and which catalyzes the oxidation of insect luciferin to yield light and the other which causes DNA transcription, and obtaining the polypeptide by transcription and subsequent translation. The insect luciferin is derived from bioluminescent insect, preferably Diptera and Coleoptera (fireflies and beetles). Hybrid proteins are similarly formed by inclusion of an additional polydeoxyribonucleotide encoding for a second polypeptide such that their respective polypeptide-encoding reading frames form a continuous reading frame. Also disclosed is a method for quantitatively assaying a fluid for the presence of an unknown quantity of antigen using the hybrid proteins bonded to antibody directed against the antigen, determining luminescence after reaction of the antibody and antigen, and determining the amount of the antigens by comparison of the determined luminescence with the luminescence previously measured under equivalent conditions for equivalent hybrid proteins reacted under equivalent conditions with known amounts of the antigen.
Mutants of 2,5-diketo-D-gluconic acid reductase A, an enzyme used to produce 2-keto-L-gulonic acid, a precursor of ascorbic acid (vitamin C) are prepared by site-directed mutagenesis. These mutants have increased catalytic activity, increased expression levels, and/or enhanced temperature stability.
5583027 E N Z Y M E I N T E N D E D FOR THE F R A G M E N T A T I O N OF N-ACETYLHEPAROSAN, P R O D U C T I O N OF PREPARATIONS CONTAINING THIS E N Z Y M E A N D F R A G M E N T A T I O N PROCESS USING THIS E N Z Y M E Salome Marc Louis V; LeLong Phillipe; Tenaille d'Estais Guy Etienne Made Castanet Tolsan, FRANCE Assigned to Sanofi Elf The invention relates to an enzyme capable of fragmenting a high molecular mass N-acetylheparosan. This enzyme was obtained with the strain Escherichia coli (K5), strain SEBR 3282.
5583032 M E T H O D OF CLEAVING SPECIFIC STRANDS OF RNA Torrence Pau; Silverman Robert; Maitra Ratan; Lesiak Krystyna Silver Spring, MD, UNITED STATES Assigned to The Cleveland Clinic Foundation and National Institutes of Health
this digestion. The antigenic material thus obtained is then combined with an appropriate adjuvant.
695
5583025 E N Z Y M E S F O R THE P R O D U C T I O N OF 2-KETO-L-GULONIC ACID Lazarus Robert A; Hurle Mark; Anderson Stephen; Powers David B Millbrae, CA, UNITED STATES Assigned to Rutgers The State University of New Jersey
5583024 R E C O M B I N A N T EXPRESSION OF C O L E O P T E R A L U C I F E R A S E McElroy Marlene D; McElroy W; Helinski Donald R; Wood Keith V; De Wet Jeffrey R; Ow David W; Howell Stephen H late of La Jolla, CA, UNITED STATES Assigned to The Regents of the University of California A method is disclosed for producing a protein which expresses bioluminescance activity which involves combining two polydeoxyribonucleotides, one containing a continuous sequence of codons encoding a polypeptide which comprises a single covalently bonded molecular structure and which catalyzes the oxidation of insect luciferin to yield light and the other which causes DNA transcription, and obtaining the polypeptide by transcription and subsequent translation. The insect luciferin is derived from bioluminescent insect, preferably Diptera and Coleoptera (fireflies and beetles). Hybrid proteins are similarly formed by inclusion of an additional polydeoxyribonucleotide encoding for a second polypeptide such that their respective polypeptide-encoding reading frames form a continuous reading frame. Also disclosed is a method for quantitatively assaying a fluid for the presence of an unknown quantity of antigen using the hybrid proteins bonded to antibody directed against the antigen, determining luminescence after reaction of the antibody and antigen, and determining the amount of the antigens by comparison of the determined luminescence with the luminescence previously measured under equivalent conditions for equivalent hybrid proteins reacted under equivalent conditions with known amounts of the antigen.
Mutants of 2,5-diketo-D-gluconic acid reductase A, an enzyme used to produce 2-keto-L-gulonic acid, a precursor of ascorbic acid (vitamin C) are prepared by site-directed mutagenesis. These mutants have increased catalytic activity, increased expression levels, and/or enhanced temperature stability.
5583027 E N Z Y M E I N T E N D E D FOR THE F R A G M E N T A T I O N OF N-ACETYLHEPAROSAN, P R O D U C T I O N OF PREPARATIONS CONTAINING THIS E N Z Y M E A N D F R A G M E N T A T I O N PROCESS USING THIS E N Z Y M E Salome Marc Louis V; LeLong Phillipe; Tenaille d'Estais Guy Etienne Made Castanet Tolsan, FRANCE Assigned to Sanofi Elf The invention relates to an enzyme capable of fragmenting a high molecular mass N-acetylheparosan. This enzyme was obtained with the strain Escherichia coli (K5), strain SEBR 3282.
5583032 M E T H O D OF CLEAVING SPECIFIC STRANDS OF RNA Torrence Pau; Silverman Robert; Maitra Ratan; Lesiak Krystyna Silver Spring, MD, UNITED STATES Assigned to The Cleveland Clinic Foundation and National Institutes of Health