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5599667 Polycationic supports and nucleic acid purification separation and hybridization
PATENT ABSTRACTS
5599665 PSEUDOMONAS AERUGINOSA NUCLEIC ACIDS ENCODING EXOENZYME S ACTIVITY AND USE THEREOF PSEUDOMONAS
IN DETECTING AERUGINOSA
INFECTION Barbieri Joseph T; Frank Data W; Kulich Scott M New Berlin, WI, UNITED STATES Assigned to MCW Research Foundation A genetic construct containing a coding region for exoenzyme S activity from Pseudomonas aeruginosa is disclosed. An essentially pure protein preparation of the 49 kDa form of exoenzyme S is also disclosed. The protein product of the genetic construct may be used to modify the RAS protein function in mammalian carcinomas, used as a vaccine, or used to diagnose Pseudomonas aeruginosa infection.
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5599667 POLYCATIONIC SUPPORTS AND NUCLEIC ACID PURIFICATION SEPARATION AND HYBRIDIZATION Arnold Lyle J; Nelson Norman C; Reynolds Mark A; Waldrop Alexander A San Diego, CA, UNITED STATES Assigned to Gon-Probe Incorporated Described herein is the use of polycationic solid supports in the purification of nucleic acids from solutions containing contaminants. The nucleic acids non-covalently bind to the support without significant binding of contaminants permitting their separation from the contaminants. The bound nucleic acids can be recovered from the support. Also described is the use of the supports as a means to separate polynucleotides and hybrids thereof with a nucleotide probe from unhybridized probe. Assays for target nucleotide sequences are described which employ this separation procedure.
5599668 LIGHT SCATTERING OPTICAL WAVEGUIDE METHOD FOR DETECTING BINDING
5599666 ALLELIC LADDERS FOR SHORT TANDEM REPEAT LOCI
Schumm James W; Puers Christoph Madison, WI, UNITED STATES Assigned to Promega Corporation The present invention is directed to an assay system, a kit and a process for detecting at least one short tandem repeat sequence from DNA at a specific locus utilizing an allelic ladder containing at least two short tandem repeat sequences of the same lengths as two or more known alleles for the locus.
SPECIFIC EVENTS
Stimpson Donald I; Gordon Julian; Hoijer Joanell V Gurnee, IL, UNITED STATES Assigned to Abbott Laboratories A waveguide binding assay method involves detecting the scattering of light directed into the waveguide, the scattering being the result of scattering labels specifically bound to the waveguide within the penetration depth of an evanescent wave. The waveguide may be transparent plastic or glass and the binding is typically by oligonucleotide hybridization or immunological capture. Light scattering labels include colloidal metals or non-metals, including gold, selenium and latex. A light absorbing member consisting of dye or concentrated particles may also be employed to enhance signal. Real-time binding and dissociation can be monitored visually or by video imaging, such as
5599665 PSEUDOMONAS AERUGINOSA NUCLEIC ACIDS ENCODING EXOENZYME S ACTIVITY AND USE THEREOF PSEUDOMONAS
IN DETECTING AERUGINOSA
INFECTION Barbieri Joseph T; Frank Data W; Kulich Scott M New Berlin, WI, UNITED STATES Assigned to MCW Research Foundation A genetic construct containing a coding region for exoenzyme S activity from Pseudomonas aeruginosa is disclosed. An essentially pure protein preparation of the 49 kDa form of exoenzyme S is also disclosed. The protein product of the genetic construct may be used to modify the RAS protein function in mammalian carcinomas, used as a vaccine, or used to diagnose Pseudomonas aeruginosa infection.
723
5599667 POLYCATIONIC SUPPORTS AND NUCLEIC ACID PURIFICATION SEPARATION AND HYBRIDIZATION Arnold Lyle J; Nelson Norman C; Reynolds Mark A; Waldrop Alexander A San Diego, CA, UNITED STATES Assigned to Gon-Probe Incorporated Described herein is the use of polycationic solid supports in the purification of nucleic acids from solutions containing contaminants. The nucleic acids non-covalently bind to the support without significant binding of contaminants permitting their separation from the contaminants. The bound nucleic acids can be recovered from the support. Also described is the use of the supports as a means to separate polynucleotides and hybrids thereof with a nucleotide probe from unhybridized probe. Assays for target nucleotide sequences are described which employ this separation procedure.
5599668 LIGHT SCATTERING OPTICAL WAVEGUIDE METHOD FOR DETECTING BINDING
5599666 ALLELIC LADDERS FOR SHORT TANDEM REPEAT LOCI
Schumm James W; Puers Christoph Madison, WI, UNITED STATES Assigned to Promega Corporation The present invention is directed to an assay system, a kit and a process for detecting at least one short tandem repeat sequence from DNA at a specific locus utilizing an allelic ladder containing at least two short tandem repeat sequences of the same lengths as two or more known alleles for the locus.
SPECIFIC EVENTS
Stimpson Donald I; Gordon Julian; Hoijer Joanell V Gurnee, IL, UNITED STATES Assigned to Abbott Laboratories A waveguide binding assay method involves detecting the scattering of light directed into the waveguide, the scattering being the result of scattering labels specifically bound to the waveguide within the penetration depth of an evanescent wave. The waveguide may be transparent plastic or glass and the binding is typically by oligonucleotide hybridization or immunological capture. Light scattering labels include colloidal metals or non-metals, including gold, selenium and latex. A light absorbing member consisting of dye or concentrated particles may also be employed to enhance signal. Real-time binding and dissociation can be monitored visually or by video imaging, such as