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823 Noradrenergic and cholinergic interaction in the rat amygdala: A immunohistochemical study
s107
822
INHIBITION
OF THE NEURONAL
TRANSMISSION
BY DYE-MEDIATED
PHO-
TOOXIDATION Department of Neuroscience, Osaka Bioscience Institute. 6-2-4 Furuedai, Suita-shi, Osaka 565. Japan’. Subfemtomole Biorecognition Project, Japan Science and Technology Corporation’ YOSKY KATAOKA’,
HIROSHI
MORII’.2,
YASUYOSHI
WATANABEll’
The effect of reactive oxygen species on neuronal transmission was studied in rat hippocampal slices. Reactive oxygen species were generated at defined regions of the slices using dye-mediated photooxidation technique. Under the extracellular recording condition, the photo-activation of dye added to the external solution decreased the field EPSP amplitude on the dose dependent manner, and suppressed the generation of population spike in postsynaptic neurons, without affecting the afferent volley. This study indicates that reactive oxygen species tend to attack the synaptic transmission. By use of this photooxidation method, it will be possible to oxidize the neural tissue in the brain and to control the neuronal activity.
823 Dept. Physioll, 930-01, Japan2
NORADRENERGIC AND CHOLINERGIC INTERACTION DALA: A IMMUNOHISTOCHEMICAL STUDY Dept. Anatomy, Fat. Med., Toyama Med. & Pharmaceu.
IN THE RAT XMYG-
Univ., Sugitani 2630, Toyama
RUIXI LI’, HISAO NISHIJO’, QUAN-XIN WANG” 1 TERUKO UWANO’, OSAMU OHTANIz, TAKETOSHI ONO’ Interaction between noradrenergic (NA) and cholinergic (,4Ch) systems has been pharmacologically implicated in the amygdala (AM). In the present study, distribution of the NA and ACh fibers in the rat AM was analyzed by means of the immunohistochemical method using the anti-dopamine+hydroxylase (DBH) a,nd anti-choline acetyltransferase (ChAT) antibodies. The most dense NA fibers were found in the posterior basolateral nucleus and medial division of the central nucleus, while the most dense ACh fibers, in the basolateral nucleus. The confocal laser microscopic study demonstrated that noradrenergic fibers and varicosities were often located in juxtaposition to the choline&c fibers, varicosities, and neurons in basolateral, cortical and central nuclei. The electron microscopic study also indicated the results consistent with those by a confocal laser microscopy. The present results provide morphological evidence for interaction between NA and ACh systems in the AM.
824
SPATIOTEMPORAL CODINGS OF AROMATIC AMINE NEUROTRANSMITTER METABOLISMS IN THE RAT BRAIN: INTRINSIC STRUCTURE, EVOKED RESPONSES, AND PERTURBATION
BY SE
The Department of Biomedical Chemistry, Institute of International Health, The University Graduate School of Medicine, 7-3-l Hongo, Bunkyo-ku, Tokyo 113, Japan KATSUJI TAKAI, EIKO NAKAMARU, KOZO HAMADA, HIROYUKI MIYAMOTO, NOBUKAZU KAKUI, HIROYUKI HOSHIMOTO, RAMON DEL FIERRO
of Tokyo.
KOJI TSUKADA,
Our cumulative microdissection/microdialysis studies showed that the focal steady state levels/turnovers of serotonin/norepinephrine/dopamine as neurotransmitters of divergent, pancerebral modulator systems, were not those with a modest circadian oscillation as previously thought, but exhibited clear dynamic codings such as high/low level transitions and recurrent/non-recurrent fluctuations with various dynamic ranges/phases/ periodicities/regional specificity. These parameters collectively provided spatiotemporal codings and new paradigms on temporal/interregional/intersystemic correlations across the brain, mapping of evoked.responses lead to a new regional paradigm on the differential expression of multiple transmitter-release mechanisms, and metabolic responses toward pancerebral serotonin depletion were related to the collapse of circadian transition of the brain keytones to a minute cycle and differential responses of focal circadian oscillations of neuronal activities.
822
INHIBITION
OF THE NEURONAL
TRANSMISSION
BY DYE-MEDIATED
PHO-
TOOXIDATION Department of Neuroscience, Osaka Bioscience Institute. 6-2-4 Furuedai, Suita-shi, Osaka 565. Japan’. Subfemtomole Biorecognition Project, Japan Science and Technology Corporation’ YOSKY KATAOKA’,
HIROSHI
MORII’.2,
YASUYOSHI
WATANABEll’
The effect of reactive oxygen species on neuronal transmission was studied in rat hippocampal slices. Reactive oxygen species were generated at defined regions of the slices using dye-mediated photooxidation technique. Under the extracellular recording condition, the photo-activation of dye added to the external solution decreased the field EPSP amplitude on the dose dependent manner, and suppressed the generation of population spike in postsynaptic neurons, without affecting the afferent volley. This study indicates that reactive oxygen species tend to attack the synaptic transmission. By use of this photooxidation method, it will be possible to oxidize the neural tissue in the brain and to control the neuronal activity.
823 Dept. Physioll, 930-01, Japan2
NORADRENERGIC AND CHOLINERGIC INTERACTION DALA: A IMMUNOHISTOCHEMICAL STUDY Dept. Anatomy, Fat. Med., Toyama Med. & Pharmaceu.
IN THE RAT XMYG-
Univ., Sugitani 2630, Toyama
RUIXI LI’, HISAO NISHIJO’, QUAN-XIN WANG” 1 TERUKO UWANO’, OSAMU OHTANIz, TAKETOSHI ONO’ Interaction between noradrenergic (NA) and cholinergic (,4Ch) systems has been pharmacologically implicated in the amygdala (AM). In the present study, distribution of the NA and ACh fibers in the rat AM was analyzed by means of the immunohistochemical method using the anti-dopamine+hydroxylase (DBH) a,nd anti-choline acetyltransferase (ChAT) antibodies. The most dense NA fibers were found in the posterior basolateral nucleus and medial division of the central nucleus, while the most dense ACh fibers, in the basolateral nucleus. The confocal laser microscopic study demonstrated that noradrenergic fibers and varicosities were often located in juxtaposition to the choline&c fibers, varicosities, and neurons in basolateral, cortical and central nuclei. The electron microscopic study also indicated the results consistent with those by a confocal laser microscopy. The present results provide morphological evidence for interaction between NA and ACh systems in the AM.
824
SPATIOTEMPORAL CODINGS OF AROMATIC AMINE NEUROTRANSMITTER METABOLISMS IN THE RAT BRAIN: INTRINSIC STRUCTURE, EVOKED RESPONSES, AND PERTURBATION
BY SE
The Department of Biomedical Chemistry, Institute of International Health, The University Graduate School of Medicine, 7-3-l Hongo, Bunkyo-ku, Tokyo 113, Japan KATSUJI TAKAI, EIKO NAKAMARU, KOZO HAMADA, HIROYUKI MIYAMOTO, NOBUKAZU KAKUI, HIROYUKI HOSHIMOTO, RAMON DEL FIERRO
of Tokyo.
KOJI TSUKADA,
Our cumulative microdissection/microdialysis studies showed that the focal steady state levels/turnovers of serotonin/norepinephrine/dopamine as neurotransmitters of divergent, pancerebral modulator systems, were not those with a modest circadian oscillation as previously thought, but exhibited clear dynamic codings such as high/low level transitions and recurrent/non-recurrent fluctuations with various dynamic ranges/phases/ periodicities/regional specificity. These parameters collectively provided spatiotemporal codings and new paradigms on temporal/interregional/intersystemic correlations across the brain, mapping of evoked.responses lead to a new regional paradigm on the differential expression of multiple transmitter-release mechanisms, and metabolic responses toward pancerebral serotonin depletion were related to the collapse of circadian transition of the brain keytones to a minute cycle and differential responses of focal circadian oscillations of neuronal activities.