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8702779 Idiotypic-antigenic conjunction binding assay
406
PATENT ABSTRACTS
blood plasma or serum components (90) and impermeable to blood cellular and particulate matter. A second overlay (21) may be superposed with the semipermeable membrane (13) over the reactive areas (31, 91 ) to receive a blood sample (89) and meter and distribute that sample (89) uniformly to the semipermeable membrane (13) which in turn passes the plasma components (90) uniformly to the reactive areas (31, 91). The overlays (13. 21) are subsequently separable from the reactive areas (31,91 ) to remove the cellular components and particulate matter exposing the reactive areas (31, 91) to inspection by. for example, color comparison to standard charts. 8702775 SYNTHETIC PEPTIDES AND USE FOR DIAGNOSIS AND VACCINATION FOR AIDS AND ARC Ronald C KENNEDY, Gordon R DREESMAN, Myron ESSEX assigned to SOUTHWEST FOUNDATION FOR BIOMEDICAL RESEARCH; PRESIDENT AND FELLOWS OF HARVARD COLLEGE Composition and method for detection of and vaccination against the viral causative agents of AIDS and ARC. The composition is a synthetic peptide, the amino acid sequence of which is sufficiently homologous to the amino acid sequence of the gp 41 and gp 120 subunits of the gp 160 envelope glycoprotein of HTLV-III. LAV or ARC to produce an immunogenic response in a patient and which has a hydrophilic region therein. The composition may be conjugated to an appropriate carrier for use as a vaccine against AIDS and ARC or bound to the ligand of a specific binding pair, contacted with a sample of biological fluid from a patient suspected of having contracted AIDS or ARC and thereafter contacted with the anti-ligand of the specific binding pair for detection of any antibodies against the viral causative agents of AIDS or ARC which may be present in the sample. 8702776 HYBRIDOMA TUMOR CELL LINES AND THEIR MONOCLONAL ANTIBODIES TO THAUMATIN Pradip GHOSH-DASTIDAR assigned BEATRICE COMPANIES INC:
to
Murine-derived hybridoma tumor cell lines and monoclonal anti-thaumatin antibody substances produced by these cell lines. The monoclona] antibody substances may be used alone or in combination in immunological procedures for isolation of thaumatin and for quantitavive detection of thaumatin in fluid samples. 8702779 IDIOTYPIC-ANTIGENIC CONJUNCTION BINDING ASSAY Miles Gerald HOSSOM assignedto MUREX CORPORATION A method for detection or quantitation of an analyte of interest suspected of being in a specimen comprising (a) contacting a first antibody with a sample suspected of containing the analyte in such a manner that said first antibody reacts with any of the analyte present in the specimen to form a first complex; (b) contacting with any of the first complex a second antibody which recognizes and binds preferentially to the juncture of the idiotypic determ~aant of the first antibody and the antigenic determinant of the analyte of the first complex to form a second complex; and (c) determining the presence or amount of the second complex as an indication of the presence or amount of the analyte of interest in the specimen.
8702781 METHOD OF ASSAYING THE PRESENCE OF CANCER CELLS Donald R SENGER, Donald R SENGER assigned to THE BETH ISRAEL HOSPITAL ASSOCIATION Method to detect and/or determine the presence of cancer cells in mammals, including humans, and to monitor the progress of treatment for cancer. A human protein marker, the assaymarker, has been ascertained of the molecular weight 70,000-74,000, which is secreted by cancer cells at levels 10-fold or greater than that observed with normal cells. An assay is described where the assay-marker or an antigenically analogous protein, the analog-marker, is used to prepare antibodies to the assay-marker. The antibodies are then reacted with blood or serum samples to determine the level of the assaymarker in the sample. Thus, the assay essentially comprises (1) providing an antibody to the protein described above (2) reacting the antibody of
PATENT ABSTRACTS
blood plasma or serum components (90) and impermeable to blood cellular and particulate matter. A second overlay (21) may be superposed with the semipermeable membrane (13) over the reactive areas (31, 91 ) to receive a blood sample (89) and meter and distribute that sample (89) uniformly to the semipermeable membrane (13) which in turn passes the plasma components (90) uniformly to the reactive areas (31, 91). The overlays (13. 21) are subsequently separable from the reactive areas (31,91 ) to remove the cellular components and particulate matter exposing the reactive areas (31, 91) to inspection by. for example, color comparison to standard charts. 8702775 SYNTHETIC PEPTIDES AND USE FOR DIAGNOSIS AND VACCINATION FOR AIDS AND ARC Ronald C KENNEDY, Gordon R DREESMAN, Myron ESSEX assigned to SOUTHWEST FOUNDATION FOR BIOMEDICAL RESEARCH; PRESIDENT AND FELLOWS OF HARVARD COLLEGE Composition and method for detection of and vaccination against the viral causative agents of AIDS and ARC. The composition is a synthetic peptide, the amino acid sequence of which is sufficiently homologous to the amino acid sequence of the gp 41 and gp 120 subunits of the gp 160 envelope glycoprotein of HTLV-III. LAV or ARC to produce an immunogenic response in a patient and which has a hydrophilic region therein. The composition may be conjugated to an appropriate carrier for use as a vaccine against AIDS and ARC or bound to the ligand of a specific binding pair, contacted with a sample of biological fluid from a patient suspected of having contracted AIDS or ARC and thereafter contacted with the anti-ligand of the specific binding pair for detection of any antibodies against the viral causative agents of AIDS or ARC which may be present in the sample. 8702776 HYBRIDOMA TUMOR CELL LINES AND THEIR MONOCLONAL ANTIBODIES TO THAUMATIN Pradip GHOSH-DASTIDAR assigned BEATRICE COMPANIES INC:
to
Murine-derived hybridoma tumor cell lines and monoclonal anti-thaumatin antibody substances produced by these cell lines. The monoclona] antibody substances may be used alone or in combination in immunological procedures for isolation of thaumatin and for quantitavive detection of thaumatin in fluid samples. 8702779 IDIOTYPIC-ANTIGENIC CONJUNCTION BINDING ASSAY Miles Gerald HOSSOM assignedto MUREX CORPORATION A method for detection or quantitation of an analyte of interest suspected of being in a specimen comprising (a) contacting a first antibody with a sample suspected of containing the analyte in such a manner that said first antibody reacts with any of the analyte present in the specimen to form a first complex; (b) contacting with any of the first complex a second antibody which recognizes and binds preferentially to the juncture of the idiotypic determ~aant of the first antibody and the antigenic determinant of the analyte of the first complex to form a second complex; and (c) determining the presence or amount of the second complex as an indication of the presence or amount of the analyte of interest in the specimen.
8702781 METHOD OF ASSAYING THE PRESENCE OF CANCER CELLS Donald R SENGER, Donald R SENGER assigned to THE BETH ISRAEL HOSPITAL ASSOCIATION Method to detect and/or determine the presence of cancer cells in mammals, including humans, and to monitor the progress of treatment for cancer. A human protein marker, the assaymarker, has been ascertained of the molecular weight 70,000-74,000, which is secreted by cancer cells at levels 10-fold or greater than that observed with normal cells. An assay is described where the assay-marker or an antigenically analogous protein, the analog-marker, is used to prepare antibodies to the assay-marker. The antibodies are then reacted with blood or serum samples to determine the level of the assaymarker in the sample. Thus, the assay essentially comprises (1) providing an antibody to the protein described above (2) reacting the antibody of