A study on the innervation of the urinary bladder of the cat

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SENSORY INNERVATlON OF URETER IN YOUNG DOG USING RETROGRADE AND ANTEROGRADE AXONAL TRANSPORT OF WGA-HRP AND IMMUNOHISTOCHEMISTRY.

M. Morimoto, K.,

T.,

Department of Anesthesiology,

Saga medical School, Nabeshima, Saga, 840-01, Japan

AIM OF lNVFS’X&UQ@l:

Foster 241 BLUE Th-Fri Exhibit Hall Abs No

781

This study was undertaken to evaluate the sensory innervation of the ureter by

morphological method. J&&Q&:

Young female dogs were usad for this study. For the operation and fixation, the experimental animals

were deeply anesthetized. WGA-HRP were injected into the ureter or spinal gannglia. Forthe immunohisto-

chemistry,substance-P(SP) andcalcitoningene-relatedpeptide(CGRP)immunoreactive (IR) fibers were detected by ABC method. RESULTS:

The upper ureter was innervated from the ipsilateral T12-L2 spinal ganglia and the lower ureter from

the ipsilateral L3 and S2 spinal ganglia, predominantry. Following these spinal ganglia injections, labeled fibers bundles were observed in the adventitia of the ureter. In the tunica submucose and tunica propria mucosae, many labeled fibers were observed. A few labeled fibers were also seen in the epithelium. SP and CGRP-IR fibers distributed similar to those of WGA-HRP labeled fibers, whereas these IR fibers were less frequent. m:

The distribution of the spinal ganglia projection to the upper ureter was different from that of the

lower ureter. It seemed that some of these sensory fibers contained SP and/or CGRP, and many senory fibers contained another peptides.

A STUDY ON THE INNERVATION OF THE URINARY BLADDER OF THE CAT T.Kadota*, A.Ogasawara and K.Tsunekawa., Dept. of Sur. Ehime Univ., Shigenobu-Cho, Onsen-gun, Ehime, JAPAN 791-02

Poster 242 BLUE Th-Fri Exhibit Hall

AIM OF INVESTIGATION: A neuroanatomical study has been made using the HRP method to identify the pain originated IAbs from the urinary bladder. METHODS: Adult cats were anesthetized with sodium pentobarbital and HRP was injected into the wall of the urinary bladder. After 48 hrs, the animals were reanethetized and perfused, dorsal root ganglia(DRG), paravertebral ganglia(Para-G) and prevertebral ganglia(Pre-G) were dissected, and histochemical processing and analysis was performed. In addition to the untreated cat, afferent and efferent pathway were studied by transection of the hypogastric nerve(HGN) or the pelvic nerve(PN). RESULTS: Follwing injection to the urinary bladder, labeled cells were observed in the Ll-L7(24%) and Sl-Cx1(76%) DRG, bilaterally. It is possible to distinguish into two groups, one is lumbar segments and other is sacral segments. By transection of HGN, the labeled cells in the lumbar DRG distinctly decreased, and also of PN was decreased labeled cellsin the sacral DRG. Labeled cells were distributed in the Th13-Cx3 Para-G with majority of the cells in L2-S3(66-100%). Transection of HGN and PN also decreased Para-G's labeled cells. In Pre-G, most of labeled cells were seen in the inferior mesenteric ganglion. CONCLUSION: Our experimental research indicated that urinary bladder has both tracts of lumbar and sacral regions in the afferent and the efferent components that mostly independented the HGN and the PN, respectively . These fact suggest that vesical pain'and / or discomfort are involved not only sympathetic and also parasympathetic system.