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Abiotic stress tolerance and growth responses of transgenic potato (Solanum tuberosum L. cv. Kennebec) plants expressing rice Osmyb4 gene
New Biotechnology · Volume 29S · September 2012
Oral 3.5.02 Development of rapid, more automated biomarker diagnostic systems Rudi Pauwels Biocartis, Lausanne, Switzerland Addressing the growing needs to monitor a growing number and type of biomarkers, Biocartis is currently in the late stages of development of two new molecular diagnostic systems for primary use in personalized medicine but with potential utility in agro, environmental and veterinary fields. The systems were designed for maximal ease of use, minimal time to result, broad biological sample and assay versatility, and automation. The first is a high multiplex system that allows rapid and simultaneous detection of protein and nucleic acid markers in a dynamic and unique way using binary encoded microparticles and a diffusion optimized microfluidic architecture. The second is a random-access sample-in to results-out system that enables the simultaneous detection and quantitation of multiple nucleic acid (DNA and RNA) biomarkers with minimal user intervention from a variety of sample types. This platform comprises three main components, i.e. (i) single use, disposable plastic cartridges in which the entire assay procedure is carried out, that are pre-filled with all the reagents that are needed in executing the assay and which completely and hermetically sealed once the sample is introduced; (ii) a set of instruments, each of which is designed to receive one cartridge and that carries out a pre-defined assay protocol and (iii) a console, which is essentially a touch screen operated computer that is used for all user interactions such as initiating an assay or reviewing an assay result and that enables 2-way communication with external IT systems for data exchange. http://dx.doi.org/10.1016/j.nbt.2012.08.067 Oral 3.5.03 Abiotic stress tolerance and growth responses of transgenic potato (Solanum tuberosum L. cv. Kennebec) plants expressing rice Osmyb4 gene Gülsüm Aydın 1,2,∗ , Meral Yücel 2 , Hüseyin Avni Öktem 2 1
Department of Biology, Selcuk University, 42151, Konya, Turkey Department of Biology, Middle East Technical University, 06800, Ankara, Turkey 2
MYB transcription factors are involved in diverse biochemical and physiological processes such as regulation of secondary metabolism, meristem formation, cell morphogenesis and floral and seed development. They are also involved in certain defence and stress responses and in hormone signalling. In the present study, we developed transgenic potato (Solanum tuberosum L. cv. Kennebec) expressing Oryza sativa myb4 gene, encoding MYB4 transcription factor, driven by either CaMV35S constitutive promoter or cold inducible COR15a promoter. The transformation frequencies were 37.6% (pCORmyb4) and 27.5% (pCaMVmyb4). A total of four pCOR lines and three pCaMV lines were selected randomly for bioassays. The transgenic plants were not growth retarded and there was no significant difference (p < 0.05) in their
tuber yield compared to non-transgenic wild type plants. Although chlorophyll a, chlorophyll b and anthocyanin contents of transgenic lines were similar to wild type, one of the pCaMV lines had a significantly higher carotenoid content. The total sugar contents of two lines were also significantly higher. Transgenic plants were also evaluated for abiotic stress tolerances. There was no significant difference in boron and freezing tolerances of wild type and transgenic lines. However a pCOR line and a pCaMV line were more salt tolerant than wild type with respect to growth parameters. These results demonstrated that heterologous expression of myb4 in potato affects carotenoid and total sugar content and also enhances salt stress tolerance. The results also showed that effect of myb4 expression might be different in each transgenic line under the conditions tested. Keywords: Abiotic stress; Agrobacterium-mediated gene transfer; Carotenoid; myb4; Potato http://dx.doi.org/10.1016/j.nbt.2012.08.068 Oral 3.5.04 Legume biotechnology: molecular basis of floral transition in soybean Prem L. Bhalla ∗ , Annie C.E. Wong, Chol-Hee Jung, Mohan B. Singh Plant Molecular Biology and Biotechnology Laboratory, ARC Centre of Excellence for Integrative Legume Research, The University of Melbourne, Parkville, Victoria 3010, Australia Flowering and seed set underpin most of the agriculture production. The knowledge on the molecular control of flowering is critical for the breeding of climate change resilient crop varieties. Most of our current understanding of the molecular basis of flower initiation and development is based on using Arabidopsis as a model system. Since legumes have unique vegetative and floral complexities, the knowledge from Arabidopsis cannot be easily extrapolated directly to legumes. Here, we address this challenge by using genomics approaches to examine flowering regulatory genes in the soybean, a major oilseed legume crop used for human and animal feed. Soybean is distributed broadly across latitudes and is cultivated as different maturity groups, with each having a narrow range of latitudinal adaptation. Unlike Arabidopsis, soybean can undergo a reversion of flowering when plants are shifted from flowering inductive to non-inductive conditions. In addition, soybean also follows a floral developmental plan that is distinct from that of Arabidopsis. Therefore, an understanding of the molecular mechanisms underlying these soybean traits is of fundamental and practical interest. Our study provides an essential genomic resource for functional analyses of the soybean flowering pathway, facilitating future biotechnology research and efforts into breeding robust high-yielding crop varieties. http://dx.doi.org/10.1016/j.nbt.2012.08.069
www.elsevier.com/locate/nbt S27
Oral 3.5.02 Development of rapid, more automated biomarker diagnostic systems Rudi Pauwels Biocartis, Lausanne, Switzerland Addressing the growing needs to monitor a growing number and type of biomarkers, Biocartis is currently in the late stages of development of two new molecular diagnostic systems for primary use in personalized medicine but with potential utility in agro, environmental and veterinary fields. The systems were designed for maximal ease of use, minimal time to result, broad biological sample and assay versatility, and automation. The first is a high multiplex system that allows rapid and simultaneous detection of protein and nucleic acid markers in a dynamic and unique way using binary encoded microparticles and a diffusion optimized microfluidic architecture. The second is a random-access sample-in to results-out system that enables the simultaneous detection and quantitation of multiple nucleic acid (DNA and RNA) biomarkers with minimal user intervention from a variety of sample types. This platform comprises three main components, i.e. (i) single use, disposable plastic cartridges in which the entire assay procedure is carried out, that are pre-filled with all the reagents that are needed in executing the assay and which completely and hermetically sealed once the sample is introduced; (ii) a set of instruments, each of which is designed to receive one cartridge and that carries out a pre-defined assay protocol and (iii) a console, which is essentially a touch screen operated computer that is used for all user interactions such as initiating an assay or reviewing an assay result and that enables 2-way communication with external IT systems for data exchange. http://dx.doi.org/10.1016/j.nbt.2012.08.067 Oral 3.5.03 Abiotic stress tolerance and growth responses of transgenic potato (Solanum tuberosum L. cv. Kennebec) plants expressing rice Osmyb4 gene Gülsüm Aydın 1,2,∗ , Meral Yücel 2 , Hüseyin Avni Öktem 2 1
Department of Biology, Selcuk University, 42151, Konya, Turkey Department of Biology, Middle East Technical University, 06800, Ankara, Turkey 2
MYB transcription factors are involved in diverse biochemical and physiological processes such as regulation of secondary metabolism, meristem formation, cell morphogenesis and floral and seed development. They are also involved in certain defence and stress responses and in hormone signalling. In the present study, we developed transgenic potato (Solanum tuberosum L. cv. Kennebec) expressing Oryza sativa myb4 gene, encoding MYB4 transcription factor, driven by either CaMV35S constitutive promoter or cold inducible COR15a promoter. The transformation frequencies were 37.6% (pCORmyb4) and 27.5% (pCaMVmyb4). A total of four pCOR lines and three pCaMV lines were selected randomly for bioassays. The transgenic plants were not growth retarded and there was no significant difference (p < 0.05) in their
tuber yield compared to non-transgenic wild type plants. Although chlorophyll a, chlorophyll b and anthocyanin contents of transgenic lines were similar to wild type, one of the pCaMV lines had a significantly higher carotenoid content. The total sugar contents of two lines were also significantly higher. Transgenic plants were also evaluated for abiotic stress tolerances. There was no significant difference in boron and freezing tolerances of wild type and transgenic lines. However a pCOR line and a pCaMV line were more salt tolerant than wild type with respect to growth parameters. These results demonstrated that heterologous expression of myb4 in potato affects carotenoid and total sugar content and also enhances salt stress tolerance. The results also showed that effect of myb4 expression might be different in each transgenic line under the conditions tested. Keywords: Abiotic stress; Agrobacterium-mediated gene transfer; Carotenoid; myb4; Potato http://dx.doi.org/10.1016/j.nbt.2012.08.068 Oral 3.5.04 Legume biotechnology: molecular basis of floral transition in soybean Prem L. Bhalla ∗ , Annie C.E. Wong, Chol-Hee Jung, Mohan B. Singh Plant Molecular Biology and Biotechnology Laboratory, ARC Centre of Excellence for Integrative Legume Research, The University of Melbourne, Parkville, Victoria 3010, Australia Flowering and seed set underpin most of the agriculture production. The knowledge on the molecular control of flowering is critical for the breeding of climate change resilient crop varieties. Most of our current understanding of the molecular basis of flower initiation and development is based on using Arabidopsis as a model system. Since legumes have unique vegetative and floral complexities, the knowledge from Arabidopsis cannot be easily extrapolated directly to legumes. Here, we address this challenge by using genomics approaches to examine flowering regulatory genes in the soybean, a major oilseed legume crop used for human and animal feed. Soybean is distributed broadly across latitudes and is cultivated as different maturity groups, with each having a narrow range of latitudinal adaptation. Unlike Arabidopsis, soybean can undergo a reversion of flowering when plants are shifted from flowering inductive to non-inductive conditions. In addition, soybean also follows a floral developmental plan that is distinct from that of Arabidopsis. Therefore, an understanding of the molecular mechanisms underlying these soybean traits is of fundamental and practical interest. Our study provides an essential genomic resource for functional analyses of the soybean flowering pathway, facilitating future biotechnology research and efforts into breeding robust high-yielding crop varieties. http://dx.doi.org/10.1016/j.nbt.2012.08.069
www.elsevier.com/locate/nbt S27