Agarose gel electrophoretic analysis of Molluscum Contagiosum virus DNA

133 MODULATION OF ADENYLATE CYCLASE ACTIVITY

Efrect of Y~P~OUS kinds of agents on the moduratlon oi ikripherai blood limphocyte IIIthe patients with HerpesZoster(HZ). A.Aikawa.T.Yoshi ike. 11.Ogara Department of Dermatology. Juntendo University, School of Sdiclne Effect of Y globurincr-g:). Interferor~~ fl (IFN p). Acyciwlr (ACV), and d prcparatlon of Glycyrrhizin (GL). on expresrion uf HLA~DR antigen in snppressor/cytotoxic cells. anti-Herpes-Zostar virus titer and clinical manlft,stntlon in 31 patients with t!erpes %oster was studied. In patients treated with ACV and CL, dls;lnct reduction of neuralgia was observed as cosnpalred as that, in mtients treated with y-g1 aIld JFN-a. Furthermore. ~ncreast!d popu!atlon of HI.!-DR positive suppressori cytotoxic ceils was immcdiateiy decreased in patients treated with ACV and IX.. Incoi~trast, irxreased population of BLA-DR positive supp~.essor/ cytotoxic cells in patients with r-g1 and IFN- P was once further increased. and thegraduallr decreased hwmg B longtme for

KERATINOCYTES M.Tsutsui Medical

AGA.HOSE

GYL tiI&CTROPHORE'l!IC ANALYSIS CONTAGIOSUM VIRUS DNA

agents

OF

M.kawashima and A.Hidano. Department of T.Uemura, Dermatology, Tokyo Women's Medical Coil., Tokyo Though Molluscm Contagiosum(MC) is a common disease, only a little is known about MC virus(MCV) DNA. We report the results of the agarose gel electrophoretic analysis of MCV DNA. Materials and methods: The total DNAs, extracted from the specimen of 50 MU patients, were digested with BamHI restriction enzyms and electrophoresed on 1% agarose gels. The photographs of the gels were taken after ethidium bromide staining. nesults: row different cleavage patterns of MCV DNAs were observed and named. mL'V-a, MUV-b, MCV-c and MCV-d, respectively. MCV-a was detected in 33 cases(66$'.)and MCV-b in 15 cases(30$). All the infants were infected with either of them. In contrast, MCV-c was detected only in 47-y-o male immunosuppressed renal allograft recipient. MCV-d was also detected in 34-y-o male patient. The clinical features of MCV-c and MCV-d induced MC we‘re different from those of ordinary ones in the sizes or affected areas.

PURIFICATION AND BIOCHEMICALPROPERTIESOF FROM CANDIDA _--__

CALMODULINS

ALBICANS

C)T.Noda,M.Seishima,S.nori,Departnent Universlty,S.Nagao,Y.Nozak.a,Depart.Prlt University.

of

Dermatology,Cifu of Blochemistory,Cifu

Callllodulln from -Candida Albicans was purified by trichloacetic _~ acid prs~lpllalion and hydrophohlc interaction cilronatography.Likc other calnodullns,Candida calmodulln activated rat braln cyclic AnP-phosphodlestcrase in a Ca’*dependent manner,but its concen;rstion for half-maximal activation wras 10 tinlps that of rat brain calmodulin.From its a!obillty on SDS polyacrylarnlde gel clectrophoresis.Xr was d?terolined to be- I6;OOO (cf.rat-brain cal~bduIin~l8,000~.In ordsr to get lniornation regarding functions of this calmoduIin,we have invastlgated the rffectof the calnlodulln on the activities of cyclic AMP-phosphodiesterase.ATPasr and Protean kInas? 111 this organism,but none of these enzymes we’re sflululated by calmodulln.On the other hand.ahen callnodulin contents measurlpd in ~C~SL forIn and mycelial farm of Ce,the latter exhibited 80-fold higher amount of ralmodulin.These data suggest that the n~olerular stfuct”re of Cendida calnodulln differs from that of rat brain caImodulin,and calnodulin may play a role in the reguration of dimorphism. tha:

were

[a

Department

Asahikawa.

Islet-activating

protein(IAP).

-5*Plnicatinamide

ADP-rihosylation.

specifically

membrane

of

proteins

substrate

acrylamide

gel

molecular late

cyclase

also

investigated.

of

about

AMP accumulations that

protein(a

I

were the

-subunit)

adenylate

and

and

cyclase

pig

skin

system

responsible in

pig

toxins. one The

of

IAP-

sulfate-poly-

corresponding by

IAP of

to three

histamine-)receptor-adeny-

slices

substrate is

TOXIN)

a substrate

least

dodecyl

epidermal were

These

increased.

which

as

band

adenosine-induced

IAP-specific

pertussis at

Modification

intact

When pig

the

sodium

a single

40.000.

in

of

keratinocrtes.

by as

EPIDERHAL

Asahikaxa

dinucleotide

radiolabeled

adenosine-.

responses

epinephrine-.histamineview

one

UBS detectable

weight

Dermatology.

epidermal

electrophoresis

major(beta-adrenergic-.

the

pig

OF PIG

PROTEIN(PEATlJSSIS

Japan

adenine

the

specific

of

Hokkaido.

for

of

MOLLUSCUM

H.Iizuka.

College.

serving

normlization. These findings suggested that ACV and GL were mc~re useful for the treatment of patients with Herpes %osLw.

and

BY ISLET-ACTIVATING

keretinocytes incubated

was with

intracellular data is for

epidermal

are

consistent

inhibitory

IAP. cyclic with

GTP-binding

inhibitory

regulation

keratinocytes.

EFFECT OF GLUCOCORTICOIDS ON THE CALCIUM REGULATED DIFFERENTIATION OF THE KERATINOCYTES IN CULTURE H.Mikami, Y.Mikami, D.Sawamura and I.Hashimoto. Department of Dermatology, Hirosaki University School of Medicine, 5 Zaifu-cho Hirosaki, Japan Transalutaminase (TG) can be a marker of the calcium (Ca) The present regulated differentiation of keratinocytes in culture. study deals with alterations of TG activity with the concentration and the kinds of glucocorticoids (GC). To study the effect of GC concentration. the TG activitv was measured 2 davs after Ca switch. When the con&ration of hyd;ocortisone (HC), piedonisolone (PR) and triamcinolone acetonide (TA) was less than IO-7g/ml, the activity was hither. When the concentration of these three GCs was more than On the other hand, the activity lo-5g/ml, the activity was lower. was low at everv concentration of dexamethasone (DX). To study the effect of the k;nds of GC on the keratinacyte TG activity, the-above 4 kinds of GC were added to the cultures according to the weight ratio with which these GCs are known to exhibit the same level of The TG activity was measured 2 days after antiphlogistic potency. Cd switch. When the GC concentrations were lower, the activity was highest in HC, followed by PR, TA and DX. When the concentrations were higher, the activity was the highest in DX, followed by TA, HC and PR.

GENETIC LLAMINE

CONTROL ANTIGEN

OF DELAYED-TYPE

HYPERSENSITIVITY

N.Ishii and H. Nakajima. Department of Dermatology, University School of Medicine, Yokohama.

TO D-PENICI-

Yokohama

City

D-penicillamine, a sulfur-containing amino acid, has been employed as a therapeutic agent for nearly 20 years in the treatment of rheumatoid arthritis, cystinuria, Wilson's aisease and heavy metal intoxication For determining the mechanism of genetic control of D-penicillamine free-base-specificdelayedtype hypersensitivity(DTH) in mice, footpad swellinq response was employed. Studies with various congenii and recombinant inbred strain; of mice revealed that D-penicillamine free-base-specific DTH was controlled by the I-A subregion. The footpad swelling response showed a highly antigen-specific pattern, and revealed that D-penicillamine free base was not crossreactive with D-penicillamine disulfide or L-penicillamine, but with D-penicillamine HCl. Treatment of immune lymphoid cells with monoclonal antibodies plus complement revealed that the cells responsible for DTH transfer were Lyt-1+2-, L3T4+ and Ia- T cells. D-penicillahine often causes drug-induced autoimnune diseases. These adverse side effects caused by D-penicillamine may be explained by the presence of D-penicillamine-specific TDTH. TDTH recognizemodified D-penicillamine and self-class II antigens on the surface of APC.