Angiogenesis factor(s) activity can be modulated by gangliosides

Pharmaco~gicalResearchCommunications, VoL2~ SupplementAl988 ANGIOGBNESIS

FACTOR(S)

ACTIVITY

CAN

BE

11

MODULATED

BY

GANGLIOSIDES Marina

Ziche*,

*Department

of

#Department

of

Morbidelli*

Pharmacology

of Biomedical

Key words: The

Lucia

and G i u l i o

University

Sciences

of new c a p i l l a r y

physiological

and p a t h o l o g i c a l

inflammatory

reactions

substances

and

factors

angiogenic

but

growth

the m e c h a n i s m : b y

maintained

previously

effector

iJl

capillary

et a]

each

other

gangliosides model of

increase

grown

in

1982)

are

and b-FGF.

ug of either

by

~

vitro

the action to

we have

studied

hypothesized triggering of

sustain

several the

all

the effect

in 3Li_V_Q in the

activity

for

angiogenic

of events

Gangliosides

to

mitogen

has

We observed

We

gangliosides

a potent and

is

response

needed

up to 80% the a n g i o g e n i c

0031-6989/88/20S1001 I-2/$03.00/0

in

be

an~iogenic

that

We have

induced

to

unclear.

potent and

~s also

as a cascade

on a n g i o g e n e s i s

0.25

a

culture

experiments

by PGEI

reported

is still

is

mouulating

Several

the all p h e n o m e n o n

et al 1979)

occurs

factors"

In this

which

like

tumor growth.

potentiated b-FGF

and m o l e c u l e s

"angiogenesis

healing

migration

is

(Gospodarowioz

angiogenesis

conditions

have been

et al

cell

1986).

cells

that

tion

( Ziche

stimuli

endothelial

cornea

of Turin

feature

and

that PGEI

endothelial

(Alessandri

process.

and

is an important

and or inhibited

shown

vivo

angiogenic

activity

Florence

University

vessel

processes,

have

of

angiogenesis/PGEl/b-FGF/gangliosides

growth

induced,

Alessandri#

that:

mixture

of

rabbit a> addior

of low doses

GTIb of

© 1988 The Italian Pharmacological Society

Pharmaco~g~alResearchCommun~armncVo~2~Supp~ment~1988

12 PGEI

(10-50-100

like

GTlb

retained

Gangliosides rate,

rig);

the

times

c) b-FGF lower

exhibited

compared

migration

indicate

:

capillary

endothelial

to the controls experiments

molecole

in sialic

the

stimulating

work was supported

5-10

the

Our

not in

results

migration

b) as in the

activity

was more

of

in

evident

acid and disappeared

response

was

when

acid was tested

rich

in

induced

sialic

the

alone.

acid

by PGEI

for

can

probably

cell motility.

Ziche H, Jones J, Gullino Alessandri G, Raju K, 165,1986 Gospodarowicz D, 8ialecki 514,1979

This

at doses

up to 5-8 fold compared

260~18);

gangliosiden

endothelial

not

of gangliosides

cells.

potentiate

of it or sialic

angiogenic

were

action

the effect

endothelial

(39~4 versus

that

its

to

and growth

We have also studied

cells in y _ ~

was deprived

concluded

modulate

assay

gangliosides

rich

but

by GTIb.

the potentiating

gangliosides

of vessel

activity

acid

compared

or asialo ganglioside

PGEI

chamber

of capillary a)

activity

angiogenic

to

potentiate

in the Boyden

rich in sialio

in terms of number

mixture

drammatically

We

strongest

but sialic acid alone

active;

on

b) gangliosides

PM: JNCI 69:475-482,1982 Gullino PM: Invas Hetast H, Thakral

TK:

6:145-

Exp Eye Res 28:501-

by MPI 60% and AIRC