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Antibacterial activity of Leonurus sibiricus aerial parts
Fitoterapia 77 (2006) 316 – 317 www.elsevier.com/locate/fitote
Short report
Antibacterial activity of Leonurus sibiricus aerial parts Firoj Ahmed ⁎, M. Amirul Islam, M. Mustafizur Rahman Pharmacy Discipline, Khulna University, Khulna-9208, Bangladesh Received 14 July 2003; accepted 25 March 2006 Available online 11 May 2006
Abstract Different solvent extracts (carbon tetrachloride, chloroform, acetone and methanol) of Leonurus sibiricus were studied for their antibacterial activity. Carbon tetrachloride and chloroform extracts showed a broad spectrum of antibacterial activity. © 2006 Elsevier B.V. All rights reserved. Keywords: Leonurus sibiricus; Antibacterial activity
1. Plant Leonurus sibiricus L. (Labiatae): aerial parts were collected on September 2002 from the District of Pabna and identified by the experts at the National Herbarium of Bangladesh (Voucher No. 29750). 2. Uses in traditional medicine and previously reported activity The plant is a respiratory stimulant, has a curare like effect on motor endings, its roots and leaves are used as febrifuge, and leaves cause contraction of uterus [1]. In Chinese medicine, the seeds are considered to be constructive and aphrodisiac, and the dried plant is prescribed as a tonic, and general remedy in puerperal and menstrual diseases [2]. In the local traditional medicine practice, leaves are used in chronic rheumatism; their juice is antibacterial and extensively applied in psoriasis, scabies and chronic skin eruptions, and also used to relieve menstrual pain and excessive bleeding [3]. A number of research works have been performed to evaluate the biological activities, specially its effect on mammary glands [4], uterus [5], myocardial cells [6] and on blood viscosity [7]. 3. Previously isolated constituents Furanoditerpenelactones [8], guanidine derivatives [9] and alkaloids [10].
⁎ Corresponding author. Tel.: +880 41 720171 3x252; fax: +880 41 731244. E-mail address: [email protected] (F. Ahmed). 0367-326X/$ - see front matter © 2006 Elsevier B.V. All rights reserved. doi:10.1016/j.fitote.2006.03.005
F. Ahmed et al. / Fitoterapia 77 (2006) 316–317
317
Table 1 Antibacterial activity of different solvent extracts of L. sibiricus Bacteria
Zone of inhibition (mm) 500 μg/disc
Staphylococcus aureus S. epidermis Streptococcus pyogenes Escherichia coli Vibrio cholerae Shigella dysenteriae S. boydii
30 μg/disc
Methanol extract
CCl4 extract
Acetone extract
Chloroform extract
Kanamycin
– 8 – 9 – 8 9
20 20 30 25 13 16 17
– – 7 8 6 8 10
19 14 28 20 15 17 19
30 32 40 39 30 34 38
(–) no inhibition.
4. Tested material Methanol, carbon tetrachloride, acetone and chloroform extracts. 5. Studied activity Antibacterial activity by disc diffusion method [11]. 6. Used microorganisms Listed in Table 1. 7. Results Reported in Table 1. 8. Conclusions The CCl4 and chloroform extracts of L. sibiricus showed a broad spectrum antibacterial activity against all the bacteria tested. This result supports the traditional use of this plant. Acknowledgements The authors are grateful to Mr. Hasibur Rahman, Associate Professor, Biotechnology Discipline, Khulna University, Khulna-9208, Bangladesh. References [1] Ghani A. Medicinal plants of Bangladesh. Chemical constituents and uses. Dhaka: Asiatic Society of Bangladesh; 1998. p. 215. [2] Kirtikar KR, Basu BD. Indian Medicinal Plants, 2nd ed., vol. III. India: International Book Distributors; 1987. p. 2013. [3] Islam MA. Phytochemical and pharmacological screening of L. sibiricus. B Pharm project report submitted to Pharmacy Discipline. Bangladesh: Khulna University; 2003. p. 14. [4] Nagasawa H, Inatomi H, Suzuki M. Anticancer Res 1990;12:141. [5] Shi M, Chang L, He G. Zhongguo Zhong Yao Za Zhi 1995;20:173. [6] Xia YX. J Tradit Chin Med 1983;3:185. [7] Zou QZ, Bi RG, Li JM. Am J Chin Med 1989;17:65. [8] Satoh M, Satoh Y, Isobe K, Fujimoto Y. Chem Pharm Bull 2003;51:341. [9] Reuter G, Diehl HJ. Pharmazie 1971;26:777. [10] Luo SR. Zhong Yao Tong Bao 1985;10:32. [11] Bauer AW, Kirby WMM, Sherris JC, Turck M. Am J Clin Pathol 1966;45:493.
Short report
Antibacterial activity of Leonurus sibiricus aerial parts Firoj Ahmed ⁎, M. Amirul Islam, M. Mustafizur Rahman Pharmacy Discipline, Khulna University, Khulna-9208, Bangladesh Received 14 July 2003; accepted 25 March 2006 Available online 11 May 2006
Abstract Different solvent extracts (carbon tetrachloride, chloroform, acetone and methanol) of Leonurus sibiricus were studied for their antibacterial activity. Carbon tetrachloride and chloroform extracts showed a broad spectrum of antibacterial activity. © 2006 Elsevier B.V. All rights reserved. Keywords: Leonurus sibiricus; Antibacterial activity
1. Plant Leonurus sibiricus L. (Labiatae): aerial parts were collected on September 2002 from the District of Pabna and identified by the experts at the National Herbarium of Bangladesh (Voucher No. 29750). 2. Uses in traditional medicine and previously reported activity The plant is a respiratory stimulant, has a curare like effect on motor endings, its roots and leaves are used as febrifuge, and leaves cause contraction of uterus [1]. In Chinese medicine, the seeds are considered to be constructive and aphrodisiac, and the dried plant is prescribed as a tonic, and general remedy in puerperal and menstrual diseases [2]. In the local traditional medicine practice, leaves are used in chronic rheumatism; their juice is antibacterial and extensively applied in psoriasis, scabies and chronic skin eruptions, and also used to relieve menstrual pain and excessive bleeding [3]. A number of research works have been performed to evaluate the biological activities, specially its effect on mammary glands [4], uterus [5], myocardial cells [6] and on blood viscosity [7]. 3. Previously isolated constituents Furanoditerpenelactones [8], guanidine derivatives [9] and alkaloids [10].
⁎ Corresponding author. Tel.: +880 41 720171 3x252; fax: +880 41 731244. E-mail address: [email protected] (F. Ahmed). 0367-326X/$ - see front matter © 2006 Elsevier B.V. All rights reserved. doi:10.1016/j.fitote.2006.03.005
F. Ahmed et al. / Fitoterapia 77 (2006) 316–317
317
Table 1 Antibacterial activity of different solvent extracts of L. sibiricus Bacteria
Zone of inhibition (mm) 500 μg/disc
Staphylococcus aureus S. epidermis Streptococcus pyogenes Escherichia coli Vibrio cholerae Shigella dysenteriae S. boydii
30 μg/disc
Methanol extract
CCl4 extract
Acetone extract
Chloroform extract
Kanamycin
– 8 – 9 – 8 9
20 20 30 25 13 16 17
– – 7 8 6 8 10
19 14 28 20 15 17 19
30 32 40 39 30 34 38
(–) no inhibition.
4. Tested material Methanol, carbon tetrachloride, acetone and chloroform extracts. 5. Studied activity Antibacterial activity by disc diffusion method [11]. 6. Used microorganisms Listed in Table 1. 7. Results Reported in Table 1. 8. Conclusions The CCl4 and chloroform extracts of L. sibiricus showed a broad spectrum antibacterial activity against all the bacteria tested. This result supports the traditional use of this plant. Acknowledgements The authors are grateful to Mr. Hasibur Rahman, Associate Professor, Biotechnology Discipline, Khulna University, Khulna-9208, Bangladesh. References [1] Ghani A. Medicinal plants of Bangladesh. Chemical constituents and uses. Dhaka: Asiatic Society of Bangladesh; 1998. p. 215. [2] Kirtikar KR, Basu BD. Indian Medicinal Plants, 2nd ed., vol. III. India: International Book Distributors; 1987. p. 2013. [3] Islam MA. Phytochemical and pharmacological screening of L. sibiricus. B Pharm project report submitted to Pharmacy Discipline. Bangladesh: Khulna University; 2003. p. 14. [4] Nagasawa H, Inatomi H, Suzuki M. Anticancer Res 1990;12:141. [5] Shi M, Chang L, He G. Zhongguo Zhong Yao Za Zhi 1995;20:173. [6] Xia YX. J Tradit Chin Med 1983;3:185. [7] Zou QZ, Bi RG, Li JM. Am J Chin Med 1989;17:65. [8] Satoh M, Satoh Y, Isobe K, Fujimoto Y. Chem Pharm Bull 2003;51:341. [9] Reuter G, Diehl HJ. Pharmazie 1971;26:777. [10] Luo SR. Zhong Yao Tong Bao 1985;10:32. [11] Bauer AW, Kirby WMM, Sherris JC, Turck M. Am J Clin Pathol 1966;45:493.