- Email: [email protected]
Antiproliferative and apoptotic properties of vulpinic acid on A549, AGS and HeLa cells
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Abstracts / Journal of Biotechnology 256S (2017) S17–S43
microscopy (TEM), zeta potential and Fourier transform infrared spectroscopy (FTIR). The antioxidant activity of NPs was evaluated by DPPH (2,2 diphenyl-1-picrylhydrazyl) radical scavenging method. UV–vis spectroscopy of G-AgNPs and O-AgNPs showed absorption maxima at 428 and 436 nm. Zeta potential analysis demonstrated that G-AgNPs and O-AgNPs had negative charges of −3.68 mV and −9.02 mV. TEM images revealed particle sizes of 47.2 ± 14.8 nm and 7.4 ± 3 nm for G-AgNPs and 14.8 ± 3.2 nm for OAgNPs. FTIR spectrum indicated that garlic and onion extract had the ability to perform dual functions of reduction and stabilization of AgNPs. According to DPPH activity, G-AgNPs showed higher antioxidant activity than O-AgNPs. http://dx.doi.org/10.1016/j.jbiotec.2017.06.669 New combination strategy of doxorubicin and epoxomicin in biocompatibility: Biodegradable polymeric nanoparticles increase apoptosis in breast cancer cells Ertan Kucuksayan 1 , Fatih Bozkurt 2 , Tomris Ozben 1 , Perihan Kubra Cicek 2 , Aysegul Hanikoglu 1 , Mustafa Tahsin Yilmaz 2,∗ 1 Akdeniz University, Medical Faculty, Department of Biochemistry, Antalya, Turkey 2 Yildiz Technical University, Chemical and Metallurgical Engineering Faculty, Department of Food Engineering, Istanbul, Turkey
E-mail address: [email protected] (M.T. Yilmaz). In this work, we synthesized nanoparticles (NPs) for encapsulation of chemotherapeutics and investigated their cytotoxic effects on cancer and normal cells. Dox (Doxorubicin) and/or Epo loaded PLGA NPs were prepared using a modified w/o/o double emulsion phase separation method. The synthesized NPs were characterized with respect to particle size, zeta potential, particle morphology, thermogravimetry, differential scanning calorimetry and Fourier transform infrared spectroscopy. The presence of Dox and/or Epo within NPs was proven by these material characterization analyses, revealing that Dox and/or Epo could be successfully loaded into the nanoparticles. The effects of the synthesized NPs on cytotoxicity, apoptosis, apoptotic pathways, and drug resistance were also investigated on in vitro cell culture models that were created using breast cancer cells (MCF-7) and normal cells (HUVEC). As a result, Dox and Epo loaded NPs were more cytotoxic than free Dox and Epo to MCF-7 cells while they were less cytotoxic than free Dox and Epo to single layer normal HUVEC cells. The results of this work suggested that clinical projects applying nanoparticles for encapsulation of Dox and/or Epo might be carried out as a potential treatment to overcome multidrug resistance and toxicity to normal tissues. http://dx.doi.org/10.1016/j.jbiotec.2017.06.670
Pharmaceutical Biotechnology Antiproliferative and apoptotic properties of vulpinic acid on A549, AGS and HeLa cells Hamit Emre Kizil 1,∗ , Guleray Agar 2 1 Central Research Laboratories Application and Research Center, Bayburt University, Bayburt, Turkey 2 Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey
E-mail address: [email protected] (H.E. Kizil). In medicine, lichens have been used in a wide range of applications due to their antibiotic, antimycobacterial, antiviral, anti-inflammatory, analgesic, antipyretic, antiproliferative, antitumor, allergenic, enzyme inhibitory and cytotoxic effects. Vulpinic acid is a lichen secondary metabolite of Vulpicida pinastri and Letharia vulpina which have strong antibiotic effects. In our study, A549, AGS and HeLa cancer cells were cultured and then treated with different doses of vulpinic acid and the expression level of the proliferation marker topo II ␣ gene was measured. It was also checked whether p53 and caspase-3 gene expressions, which might be apoptotic markers, were also increased. As a result, concentrations of 12.5, 50 and 100 g/ml of vulpinic acid for the A549 cell line were found to be antiproliferative and apoptotic. It was also observed that the concentration of 50 g/ml for the AGS cell line and 12.5 g/ml for the HeLa cell line might be antiproliferative and apoptotic. Consequently, it can be anticipated that vulpinic acid can be used as therapeutics against human lung cancer, stomach cancer and cervical cancer. In order to obtain better results, it is necessary to measure expression levels of genes such as bcl2 and bax and to repeat all applications in vivo. http://dx.doi.org/10.1016/j.jbiotec.2017.06.671 Determination of the Antigenotoxic effects of Evernic acid against genotoxic effects of CCL4 on HUVEC lines Guleray Agar 1,∗ , Hamit Emre Kizil 2 , Selcuk Ceker 3 , Ozel Capik 1 , Bulent Sengul 4 1
Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey 2 Central Research Laboratories Application and Research Center, Bayburt University, Bayburt, Turkey 3 Department of Basic Pharmaceutical Sciences, Faculty of Pharmacy, Agri Ibrahim Cecen University, Agri, Turkey 4 Vocational School of Health Services, Bayburt University, Bayburt, Turkey E-mail address: [email protected] (G. Agar). Lichens have long been used as traditional medicines in many countries. Infections of the chemotheapathic failures and antibiotic-resistant pathogenic microorganisms have led to the search for many plant secondary metabolites in terms of their antimicrobial activity. Recent studies have begun to investigate the antigotoxic effects of synthetic, plant and fungal sources against various types of cancer. Particularly in recent years, studies with plant and fungus-derived extracts have produced promising results. Studies carried out in relation to antigenotoxic and anticancerogenic effects of lichens in the published literature have started to take place in our country and world literature since 2011. The detection of the antigenotoxic and anticancerogenic effects of lichen seconder metabolites is aimed at finding promising drug candidate molecules in different diseases. In this study, the biological
Abstracts / Journal of Biotechnology 256S (2017) S17–S43
microscopy (TEM), zeta potential and Fourier transform infrared spectroscopy (FTIR). The antioxidant activity of NPs was evaluated by DPPH (2,2 diphenyl-1-picrylhydrazyl) radical scavenging method. UV–vis spectroscopy of G-AgNPs and O-AgNPs showed absorption maxima at 428 and 436 nm. Zeta potential analysis demonstrated that G-AgNPs and O-AgNPs had negative charges of −3.68 mV and −9.02 mV. TEM images revealed particle sizes of 47.2 ± 14.8 nm and 7.4 ± 3 nm for G-AgNPs and 14.8 ± 3.2 nm for OAgNPs. FTIR spectrum indicated that garlic and onion extract had the ability to perform dual functions of reduction and stabilization of AgNPs. According to DPPH activity, G-AgNPs showed higher antioxidant activity than O-AgNPs. http://dx.doi.org/10.1016/j.jbiotec.2017.06.669 New combination strategy of doxorubicin and epoxomicin in biocompatibility: Biodegradable polymeric nanoparticles increase apoptosis in breast cancer cells Ertan Kucuksayan 1 , Fatih Bozkurt 2 , Tomris Ozben 1 , Perihan Kubra Cicek 2 , Aysegul Hanikoglu 1 , Mustafa Tahsin Yilmaz 2,∗ 1 Akdeniz University, Medical Faculty, Department of Biochemistry, Antalya, Turkey 2 Yildiz Technical University, Chemical and Metallurgical Engineering Faculty, Department of Food Engineering, Istanbul, Turkey
E-mail address: [email protected] (M.T. Yilmaz). In this work, we synthesized nanoparticles (NPs) for encapsulation of chemotherapeutics and investigated their cytotoxic effects on cancer and normal cells. Dox (Doxorubicin) and/or Epo loaded PLGA NPs were prepared using a modified w/o/o double emulsion phase separation method. The synthesized NPs were characterized with respect to particle size, zeta potential, particle morphology, thermogravimetry, differential scanning calorimetry and Fourier transform infrared spectroscopy. The presence of Dox and/or Epo within NPs was proven by these material characterization analyses, revealing that Dox and/or Epo could be successfully loaded into the nanoparticles. The effects of the synthesized NPs on cytotoxicity, apoptosis, apoptotic pathways, and drug resistance were also investigated on in vitro cell culture models that were created using breast cancer cells (MCF-7) and normal cells (HUVEC). As a result, Dox and Epo loaded NPs were more cytotoxic than free Dox and Epo to MCF-7 cells while they were less cytotoxic than free Dox and Epo to single layer normal HUVEC cells. The results of this work suggested that clinical projects applying nanoparticles for encapsulation of Dox and/or Epo might be carried out as a potential treatment to overcome multidrug resistance and toxicity to normal tissues. http://dx.doi.org/10.1016/j.jbiotec.2017.06.670
Pharmaceutical Biotechnology Antiproliferative and apoptotic properties of vulpinic acid on A549, AGS and HeLa cells Hamit Emre Kizil 1,∗ , Guleray Agar 2 1 Central Research Laboratories Application and Research Center, Bayburt University, Bayburt, Turkey 2 Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey
E-mail address: [email protected] (H.E. Kizil). In medicine, lichens have been used in a wide range of applications due to their antibiotic, antimycobacterial, antiviral, anti-inflammatory, analgesic, antipyretic, antiproliferative, antitumor, allergenic, enzyme inhibitory and cytotoxic effects. Vulpinic acid is a lichen secondary metabolite of Vulpicida pinastri and Letharia vulpina which have strong antibiotic effects. In our study, A549, AGS and HeLa cancer cells were cultured and then treated with different doses of vulpinic acid and the expression level of the proliferation marker topo II ␣ gene was measured. It was also checked whether p53 and caspase-3 gene expressions, which might be apoptotic markers, were also increased. As a result, concentrations of 12.5, 50 and 100 g/ml of vulpinic acid for the A549 cell line were found to be antiproliferative and apoptotic. It was also observed that the concentration of 50 g/ml for the AGS cell line and 12.5 g/ml for the HeLa cell line might be antiproliferative and apoptotic. Consequently, it can be anticipated that vulpinic acid can be used as therapeutics against human lung cancer, stomach cancer and cervical cancer. In order to obtain better results, it is necessary to measure expression levels of genes such as bcl2 and bax and to repeat all applications in vivo. http://dx.doi.org/10.1016/j.jbiotec.2017.06.671 Determination of the Antigenotoxic effects of Evernic acid against genotoxic effects of CCL4 on HUVEC lines Guleray Agar 1,∗ , Hamit Emre Kizil 2 , Selcuk Ceker 3 , Ozel Capik 1 , Bulent Sengul 4 1
Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey 2 Central Research Laboratories Application and Research Center, Bayburt University, Bayburt, Turkey 3 Department of Basic Pharmaceutical Sciences, Faculty of Pharmacy, Agri Ibrahim Cecen University, Agri, Turkey 4 Vocational School of Health Services, Bayburt University, Bayburt, Turkey E-mail address: [email protected] (G. Agar). Lichens have long been used as traditional medicines in many countries. Infections of the chemotheapathic failures and antibiotic-resistant pathogenic microorganisms have led to the search for many plant secondary metabolites in terms of their antimicrobial activity. Recent studies have begun to investigate the antigotoxic effects of synthetic, plant and fungal sources against various types of cancer. Particularly in recent years, studies with plant and fungus-derived extracts have produced promising results. Studies carried out in relation to antigenotoxic and anticancerogenic effects of lichens in the published literature have started to take place in our country and world literature since 2011. The detection of the antigenotoxic and anticancerogenic effects of lichen seconder metabolites is aimed at finding promising drug candidate molecules in different diseases. In this study, the biological